Isolation and identification of Bacterial flora from Catfish with antimicrobial susceptibility and herbal sensitivity

This study was carried out for isolation and identification phenotypically morphological and biochemical characteristics of bacteria infected Clarias gariepinus and genotypically by Random Amplified Polymorphic DNA (RAPD) also, its susceptibility to different antibiotics and some natural plant extract. A total 289 strains of bacteria isolates, the number of bacterial isolates differed according to the organ of isolation. The highest rate of isolation was obtained from liver (67 isolates) followed by skin (62), intestinal contents (55), kidney (47) and spleen (46) while the lowest number of isolated bacteria were recovered from gills (12). The most commonly isolated bacteria were Aeromonas Sorbia (46.7%), A.caviae (19%), A.jamdaei (8.6%), A.veronii (8.6%), Ps.aeruginosa (2.4%), Ps.fluorescens (2%), Staph.aureus (2.7%), Staph.epidermidis (1%), Vibrio alginolitcus (4.5%) and V.anguillarum (4.15%). All tested isolates were sensitive to Ciprofloxacin followed by Cefuroxime then Gentamycin, the lowest (MIC) and (MBC) were recorded for Ciprofloxacin and alcoholic Clove extract among the plant species tested, Cinnamon, Hibiscus, Nutmeg, Thymus and Rosemary against all bacterial isolates. Keywords: Antibiotic susceptibility, Herbal sensitivity, Aeromonas spp., Phenotypic, Genotypic, Catfish.