Retained placenta on the proteinogram of Holstein cows/ Retencao de placenta no proteinograma de vacas Holandesas

The aim of this study was to evaluate the influence of retained placenta on the proteinogram of Holstein cows from commercial dairy farms. Blood samples were collected from 129 animals with retained placenta (RP) and 145 animals with normal delivery and postpartum period, without any treatment, on following days: 1st day in milk (DIM), 2nd-3th, 4th-5th, 6th-7th, 8th-14th, 15th-29th, 30th-59th and 60th-90th DIM were analyzed. Protein electrophoresis were performed in acetate cellulose and sodium dodecyl sulfate polyacrilamide gel (SDS-PAGE), where 19 protein bands were observed with molecular weights between 23KDa and 187KDa. There was no influence on serum totalprotein and gamma globulins. Serum albumin remained below the normal reference values up to 90DIM. In conclusion, Holstein cows with RP have normoproteinemia with hypoalbuminemia up to 90DIM, presence of positive acute phase response by increase of haptoglobin, ceruloplasmin, acid glycoprotein, alpha and beta globulins and negative acute phase response by decreased of albumin within the first week postpartum. Key words: bovine, puerperium, acute phase proteins, SDS-PAGE. Com o objetivo de avaliar a influencia da retencao de placenta (RP) no proteinograma de femeas bovinas da raca Holandesa, de propriedades comerciais, foram utilizadas 129 vacas com RP e 145 vacas com parto epos-parto fisiologicos e sem nenhum tratamento no periodo avaliado. As amostras de sangue foram divididas nos momentos: 1odia pos-parto (DPP), 2-3, 4-5, 6-7, 8-14, 15-29, 30-59 e 60-90 DPP. O fracionamento das proteinas foi realizado por eletroforese em fita de acetato de celulose e em gel de poliacrilamida, contendo dodecil sulfato de sodio (SDS-PAGE), nas quais se avaliou o comportamento de 19 bandas proteicas identificadas pelos respectivos pesos moleculares, que variaram entre 23KDa e 187KDa. Nao houve influencia da RP na proteina serica total e gamaglobulinas. A albumina serica permaneceu abaixo dos valores de referencia ate os 90DPP nos animais com RP. Concluiu-se que vacas Holandesas com RP apresentam um quadro de normoproteinemia com hipoalbuminemia e aumento das fracoes alfaglobulinas e betaglobulinas ate os 90DPP, presenca de resposta inflamatoria de fase aguda positiva pelo significativo aumento de haptoglobina, ceruloplasmina, glicoproteina acida, e de fase aguda negativa pela diminuicao de albumina na primeira semana pos-parto. Palavras-chave: bovinos, puerperio, proteinas de fase aguda, SDS-PAGE.