Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy
In this contribution, the great potential of surface enhanced Raman spectoscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identif...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2011-07, Vol.400 (9), p.2755 |
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| container_issue | 9 |
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| container_title | Analytical and bioanalytical chemistry |
| container_volume | 400 |
| creator | Marz, Anne Winch, Bettina Misch, Petra Kiehntopf, Michael Henkel, Thomas Popp, Jurgen |
| description | In this contribution, the great potential of surface enhanced Raman spectoscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented. Keywords SERS * Microfluidic lab-on-a-chip * Enzyme activity * Thiopurine methyltransferase * 6-Mercaptopurine |
| doi_str_mv | 10.1007/00216-011-4811-z |
| format | Article |
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Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented. 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Using LOC-SERS, the enzyme activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being able to determine the TPMT activity before starting a treatment using 6-mercaptopurine, an optimized dosage can be applied to each patient and serious toxicity appearing within thiopurine treatment will be prevented. 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| ispartof | Analytical and bioanalytical chemistry, 2011-07, Vol.400 (9), p.2755 |
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| language | eng |
| recordid | cdi_gale_infotracacademiconefile_A394857412 |
| source | Springer Online Journals Complete |
| subjects | Mercaptopurine Microfluidics Raman spectroscopy Transferases |
| title | Detection of thiopurine methyltransferase activity in lysed red blood cells by means of lab-on-a-chip surface enhanced Raman spectroscopy |
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