Lead phytoavailability change driven by its speciation transformation after the addition of tea polyphenols and XANES analysis

Background and aims Metal species in rhizosphere soil profoundly influence their mobility and phytoavailability. Clarifying the speciation transformation of heavy metals helps understand their translocation and accumulation in plants. Methods Single extraction, selective sequential extraction (SSE) and X-ray absorption near-edge structure (XANES) spectroscopy were employed to investigate the speciation transformation of lead (Pb) and its influence on metal accumulation in tea plants after the addition of tea polyphenols (TPs). Results Pb content was decreased in young leaves and stems, whereas increased in roots, after TPs were amended to soil. Both SSE and XANES analysis suggested bioavailable Pb was transformed to organically bound Pb after the addition of TPs. The increased percentage of organically bound Pb might be fixed in the cell wall of plant root through a ternary complex formed between the Pb-organic matter complex and cell wall components. Therefore, Pb translocation from roots to young tissues was decreased. Conclusions Pb phytoavailability change was driven by its speciation transformation after the addition of TPs. Combined SSE and XANES spectroscopy represent powerful tools to study metal speciation transformation in plant and soil systems. Keywords XANES * Lead (Pb) * Rhizosphere * Phytoavailability * Tea polyphenols (TPs)