Molecular identification of the grapevine 'Goethe' from Urussanga with microsatellite markers/Identificacao molecular da uva 'Goethe' de Urussanga-SC por marcadores microssatelites

'Goethe' grape is a symbol of the viticulture of Urussanga region, south of the state of Santa Catarina, which is currently claiming the geographical indication of the grape and wine Goethe. Microsatellite markers are the biotechnological tool most used for molecular identification of grapevine varieties worldwide. These markers have the potential of generate unique DNA profiles for all the diverse grape genotypes. The aim of this work was to characterize two selections of the grapevine 'Goethe' from Urussanga, using microsatellite markers. Genomic DNA was extracted from young leaves and branches of nine accessions of each selection " Goethe Classica and ' Goethe Primo' maintained at one public and eight private collections of Urussanga. Ten microsatellite loci VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31 and VVMD32 were genotyped by capillary electrophoresis. Results showed that the two variants of 'Goethe' have an identical molecular profile which doesn't match any other DNA profile of grapevines previously described in literature or available in databases. The phenotypic differences observed are probably due to somatic mutations in functional regions of the genome, a phenomenon which leads to the creation of clones in grape. Index terms: Vitis, identification of cultivars, microsatellite markers, intravarietal variability. A variedade Goethe e simbolo da vitivinicultura da regiao de Urussanga, sul do Estado de Santa Catarina, a qual, atualmente, busca a Indicacao Geografica da Uva e do Vinho Goethe. Para isto, um dos requisitos necessarios e a identificacao precisa do material genetico. Os marcadores microssatelites constituem a ferramenta molecular mais utilizada para a identificacao varietal de videira em todo o mundo e tEm a capacidade de produzir um perfil genetico unico para cada material viticola. O objetivo deste trabalho foi caracterizar duas selecoes de uva 'Goethe', presentes no municipio de Urussanga, por meio de marcadores moleculares microssatelites, visando a atender aos requisitos de denominacao de origem e indicacao de procedEncia controlada. A extracao do DNA genomico foi realizada a partir de folhas jovens e ramos de nove acessos de cada selecao de 'Goethe Classica' e 'Goethe Primo' provenientes de uma colecao publica e de oito colecoes privadas da regiao de Urussanga. Dez loci microssatelites VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, VVMD31 e VVMD32 foram genotipados atraves de eletroforese