Manipulation of Interleukin-6
Hepatocellular carcinoma (HCC) is the most common liver malignancy. Early diagnosis of HCC has always been challenging. This study aims to assess the pathogenicity and the prevalence of IL-6 -174G/C (rs1800795) and TGF[beta]-1 +29C/T (rs1800470) polymorphisms in HCV-infected HCC patients. Experiment...
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| Veröffentlicht in: | PloS one 2022-10, Vol.17 (10), p.e0275834 |
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| creator | Badshah, Yasmin Shabbir, Maria Khan, Khushbukhat Fatima, Maha Majoka, Iqra Aslam, Laiba Munawar, Huda |
| description | Hepatocellular carcinoma (HCC) is the most common liver malignancy. Early diagnosis of HCC has always been challenging. This study aims to assess the pathogenicity and the prevalence of IL-6 -174G/C (rs1800795) and TGF[beta]-1 +29C/T (rs1800470) polymorphisms in HCV-infected HCC patients. Experimental strategies are integrated with computational approaches to analyse the pathogenicity of the TGF[beta]-1 +29C/T and IL-6-174 G/C polymorphisms in HCV-induced HCC. AliBaba2 was used to predict the effect of IL-6-174 G/C on transcription factor binding site in IL-6 gene. Structural changes in the mutant TGF[beta]-1 structure were determined through project HOPE. To assess the polymorphic prevalence of IL-6 -174G/C and TGF[beta]-1 +29C/T genotypes in HCC and control subjects, amplification refractory mutation system PCR (ARMS-PCR) was performed on 213 HCC and 216 control samples. GraphPad Prism version 8.0 was used for the statistical analysis of the results. In-silico analysis revealed the regulatory nature of both IL-6 -174G/C and TGF[beta]-1 +29C/T polymorphisms. ARMS-PCR results revealed that the individuals carrying TT genotype for TGF[beta]-1 gene have an increased risk of developing HCC (p |
| doi_str_mv | 10.1371/journal.pone.0275834 |
| format | Article |
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Early diagnosis of HCC has always been challenging. This study aims to assess the pathogenicity and the prevalence of IL-6 -174G/C (rs1800795) and TGF[beta]-1 +29C/T (rs1800470) polymorphisms in HCV-infected HCC patients. Experimental strategies are integrated with computational approaches to analyse the pathogenicity of the TGF[beta]-1 +29C/T and IL-6-174 G/C polymorphisms in HCV-induced HCC. AliBaba2 was used to predict the effect of IL-6-174 G/C on transcription factor binding site in IL-6 gene. Structural changes in the mutant TGF[beta]-1 structure were determined through project HOPE. To assess the polymorphic prevalence of IL-6 -174G/C and TGF[beta]-1 +29C/T genotypes in HCC and control subjects, amplification refractory mutation system PCR (ARMS-PCR) was performed on 213 HCC and 216 control samples. GraphPad Prism version 8.0 was used for the statistical analysis of the results. In-silico analysis revealed the regulatory nature of both IL-6 -174G/C and TGF[beta]-1 +29C/T polymorphisms. ARMS-PCR results revealed that the individuals carrying TT genotype for TGF[beta]-1 gene have an increased risk of developing HCC (p<0.0001, OR = 5.403, RR = 2.062) as compared to individuals with CT and CC genotype. Similarly, GC genotype carriers for IL-6 gene exhibit an increased risk of HCC susceptibility (p<0.0001, OR = 2.276, RR = 1.512) as compared to the people carrying the GG genotype. Genotype TT of TGF[beta]-1 gene and genotype GC of IL-6 gene are found to be associated with HCV-induced HCC. IL-6 polymorphism may alter its transcription that leads to its pathogenicity. TGF[beta]-1 polymorphism may alter protein structure stability.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0275834</identifier><language>eng</language><publisher>Public Library of Science</publisher><subject>Analysis ; Diagnosis ; Genetic aspects ; Genetic transcription ; Health aspects ; Interleukin-6 ; Liver cancer ; Transforming growth factors</subject><ispartof>PloS one, 2022-10, Vol.17 (10), p.e0275834</ispartof><rights>COPYRIGHT 2022 Public Library of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Badshah, Yasmin</creatorcontrib><creatorcontrib>Shabbir, Maria</creatorcontrib><creatorcontrib>Khan, Khushbukhat</creatorcontrib><creatorcontrib>Fatima, Maha</creatorcontrib><creatorcontrib>Majoka, Iqra</creatorcontrib><creatorcontrib>Aslam, Laiba</creatorcontrib><creatorcontrib>Munawar, Huda</creatorcontrib><title>Manipulation of Interleukin-6</title><title>PloS one</title><description>Hepatocellular carcinoma (HCC) is the most common liver malignancy. Early diagnosis of HCC has always been challenging. This study aims to assess the pathogenicity and the prevalence of IL-6 -174G/C (rs1800795) and TGF[beta]-1 +29C/T (rs1800470) polymorphisms in HCV-infected HCC patients. Experimental strategies are integrated with computational approaches to analyse the pathogenicity of the TGF[beta]-1 +29C/T and IL-6-174 G/C polymorphisms in HCV-induced HCC. AliBaba2 was used to predict the effect of IL-6-174 G/C on transcription factor binding site in IL-6 gene. Structural changes in the mutant TGF[beta]-1 structure were determined through project HOPE. To assess the polymorphic prevalence of IL-6 -174G/C and TGF[beta]-1 +29C/T genotypes in HCC and control subjects, amplification refractory mutation system PCR (ARMS-PCR) was performed on 213 HCC and 216 control samples. GraphPad Prism version 8.0 was used for the statistical analysis of the results. In-silico analysis revealed the regulatory nature of both IL-6 -174G/C and TGF[beta]-1 +29C/T polymorphisms. ARMS-PCR results revealed that the individuals carrying TT genotype for TGF[beta]-1 gene have an increased risk of developing HCC (p<0.0001, OR = 5.403, RR = 2.062) as compared to individuals with CT and CC genotype. Similarly, GC genotype carriers for IL-6 gene exhibit an increased risk of HCC susceptibility (p<0.0001, OR = 2.276, RR = 1.512) as compared to the people carrying the GG genotype. Genotype TT of TGF[beta]-1 gene and genotype GC of IL-6 gene are found to be associated with HCV-induced HCC. IL-6 polymorphism may alter its transcription that leads to its pathogenicity. TGF[beta]-1 polymorphism may alter protein structure stability.</description><subject>Analysis</subject><subject>Diagnosis</subject><subject>Genetic aspects</subject><subject>Genetic transcription</subject><subject>Health aspects</subject><subject>Interleukin-6</subject><subject>Liver cancer</subject><subject>Transforming growth factors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9j81Kw0AYRQdRsFbfQKErwUXSb34yM1mW4k-gpaDFbfiSzqSpw0zpJODjG9FF3Li6d3G4nEvILYWUckXnh9CfPLr0GLxJgalMc3FGJjTnLJEM-PmoX5KrGA8AGddSTsjdGn177B12bfCzYGeF78zJmf6j9Ym8JhcWXTQ3vzkl26fH7fIlWW2ei-VilTR5DomoZaV4hhozBEu1lZpLJiqKFoDtKsoBsbagUSihdqCzXJhBUVJmKysMn5KHn9kGnSlbX4dB4rNrsI-xLN5ey4ViVGkKg___7Ob9L3s_YvcGXbePwfXfX-MY_AIMi1zN</recordid><startdate>20221010</startdate><enddate>20221010</enddate><creator>Badshah, Yasmin</creator><creator>Shabbir, Maria</creator><creator>Khan, Khushbukhat</creator><creator>Fatima, Maha</creator><creator>Majoka, Iqra</creator><creator>Aslam, Laiba</creator><creator>Munawar, Huda</creator><general>Public Library of Science</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20221010</creationdate><title>Manipulation of Interleukin-6</title><author>Badshah, Yasmin ; Shabbir, Maria ; Khan, Khushbukhat ; Fatima, Maha ; Majoka, Iqra ; Aslam, Laiba ; Munawar, Huda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g990-4c6b735a8a5a0f18f683624b1af002db130aacf08a4747d08594e834612fbf4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Diagnosis</topic><topic>Genetic aspects</topic><topic>Genetic transcription</topic><topic>Health aspects</topic><topic>Interleukin-6</topic><topic>Liver cancer</topic><topic>Transforming growth factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Badshah, Yasmin</creatorcontrib><creatorcontrib>Shabbir, Maria</creatorcontrib><creatorcontrib>Khan, Khushbukhat</creatorcontrib><creatorcontrib>Fatima, Maha</creatorcontrib><creatorcontrib>Majoka, Iqra</creatorcontrib><creatorcontrib>Aslam, Laiba</creatorcontrib><creatorcontrib>Munawar, Huda</creatorcontrib><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Badshah, Yasmin</au><au>Shabbir, Maria</au><au>Khan, Khushbukhat</au><au>Fatima, Maha</au><au>Majoka, Iqra</au><au>Aslam, Laiba</au><au>Munawar, Huda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Manipulation of Interleukin-6</atitle><jtitle>PloS one</jtitle><date>2022-10-10</date><risdate>2022</risdate><volume>17</volume><issue>10</issue><spage>e0275834</spage><pages>e0275834-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hepatocellular carcinoma (HCC) is the most common liver malignancy. Early diagnosis of HCC has always been challenging. This study aims to assess the pathogenicity and the prevalence of IL-6 -174G/C (rs1800795) and TGF[beta]-1 +29C/T (rs1800470) polymorphisms in HCV-infected HCC patients. Experimental strategies are integrated with computational approaches to analyse the pathogenicity of the TGF[beta]-1 +29C/T and IL-6-174 G/C polymorphisms in HCV-induced HCC. AliBaba2 was used to predict the effect of IL-6-174 G/C on transcription factor binding site in IL-6 gene. Structural changes in the mutant TGF[beta]-1 structure were determined through project HOPE. To assess the polymorphic prevalence of IL-6 -174G/C and TGF[beta]-1 +29C/T genotypes in HCC and control subjects, amplification refractory mutation system PCR (ARMS-PCR) was performed on 213 HCC and 216 control samples. GraphPad Prism version 8.0 was used for the statistical analysis of the results. In-silico analysis revealed the regulatory nature of both IL-6 -174G/C and TGF[beta]-1 +29C/T polymorphisms. ARMS-PCR results revealed that the individuals carrying TT genotype for TGF[beta]-1 gene have an increased risk of developing HCC (p<0.0001, OR = 5.403, RR = 2.062) as compared to individuals with CT and CC genotype. Similarly, GC genotype carriers for IL-6 gene exhibit an increased risk of HCC susceptibility (p<0.0001, OR = 2.276, RR = 1.512) as compared to the people carrying the GG genotype. Genotype TT of TGF[beta]-1 gene and genotype GC of IL-6 gene are found to be associated with HCV-induced HCC. IL-6 polymorphism may alter its transcription that leads to its pathogenicity. TGF[beta]-1 polymorphism may alter protein structure stability.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0275834</doi><tpages>e0275834</tpages></addata></record> |
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| subjects | Analysis Diagnosis Genetic aspects Genetic transcription Health aspects Interleukin-6 Liver cancer Transforming growth factors |
| title | Manipulation of Interleukin-6 |
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