A method for cryopreservation and single nucleus RNA-sequencing of normal adult human interventricular septum heart tissue reveals cellular diversity and function

Background Single cell sequencing of human heart tissue is technically challenging and methods to cryopreserve heart tissue for obtaining single cell information have not been standardized. Studies published to date have used varying methods to preserve and process human heart tissue, and have gener...

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Veröffentlicht in:	BMC medical genomics 2021-06, Vol.14 (1), p.161-8, Article 161
Hauptverfasser:	Larson, Amy, Chin, Michael T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Analysis Animals Cardiomyocytes Cardiomyopathy Cardiovascular disease Cell Nucleus - genetics Composition Cryopreservation Cryopreservation - methods Cryopreservation of organs, tissues, etc Datasets Fibroblasts Functional genomics Gene expression Gene expression and regulation Genetic aspects Genetics & Heredity Genomes Genomic analysis Genomics Health aspects Heart Heart cells Heart septum Homogenization Human heart tissue Humans Identification and classification Laboratories Life Sciences & Biomedicine Methods Myocardial biology Myocardium - cytology Myocardium - metabolism Nuclei Organ donors Ribonucleic acid RNA RNA sequencing Science & Technology Septum Sequence Analysis, RNA Single nucleus RNA-sequencing Single-Cell Analysis - methods Software Swine Technical Advance Transcription Transcriptomics Ventricular Septum - cytology
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