Human cord blood
Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma.sup.null (NSG) mice engrafted with human cord blood (hCB)-CD34.sup.+ cells have been proposed to be a valuable tool to reproduce human...
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| Veröffentlicht in: | PloS one 2019-09, Vol.14 (9), p.e0217345 |
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| creator | Tanaskovic, Olga Verga Falzacappa, Maria Vittoria Pelicci, Pier Giuseppe |
| description | Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma.sup.null (NSG) mice engrafted with human cord blood (hCB)-CD34.sup.+ cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34.sup.+ cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34.sup.+ NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34.sup.+ NSG and controls suggests a poor human immune response against not compatible hAMLs. Our findings suggest that (hCB)-CD34.sup.+ NSG mice are transient and/or incomplete carriers of the human immune system and, therefore, represent a suboptimal tool to study the interaction between tumor and immune cells. |
| doi_str_mv | 10.1371/journal.pone.0217345 |
| format | Article |
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The NOD-scidIL2Rgamma.sup.null (NSG) mice engrafted with human cord blood (hCB)-CD34.sup.+ cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34.sup.+ cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34.sup.+ NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34.sup.+ NSG and controls suggests a poor human immune response against not compatible hAMLs. 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The NOD-scidIL2Rgamma.sup.null (NSG) mice engrafted with human cord blood (hCB)-CD34.sup.+ cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34.sup.+ cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34.sup.+ NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34.sup.+ NSG and controls suggests a poor human immune response against not compatible hAMLs. Our findings suggest that (hCB)-CD34.sup.+ NSG mice are transient and/or incomplete carriers of the human immune system and, therefore, represent a suboptimal tool to study the interaction between tumor and immune cells.</description><subject>Acute myelocytic leukemia</subject><subject>Bone marrow transplantation</subject><subject>Cancer research</subject><subject>Care and treatment</subject><subject>Development and progression</subject><subject>Health aspects</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Myeloid leukemia</subject><subject>Parenting</subject><subject>Surgery</subject><subject>Tumors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFzL9LAzEYxvEgCtZW8A9w6CQ43JnfdxlLUVsoFGxxPd7k3lxbYiLNHfjnO-hwTk7Pd_jwEHLPaMlExZ5OaThHCOVnilhSzioh1QWZMCN4oTkVl6O-Jjc5nyhVotZ6Qu5WwwfEuUvndm5DSu2MXHkIGW9_d0r2L8_75arYbF_Xy8Wm6IypCwkoJXp01GlQnCnpaouWes51awznFjwHyxS1rausQ5DaozKglPBojZiSx5_bDgI2x-hS7PGr72DIuVnv3pqFplRyWfH6H7t9_2sfRvaAEPpDTmHojynmMfwGT-1ZgA</recordid><startdate>20190919</startdate><enddate>20190919</enddate><creator>Tanaskovic, Olga</creator><creator>Verga Falzacappa, Maria Vittoria</creator><creator>Pelicci, Pier Giuseppe</creator><general>Public Library of Science</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20190919</creationdate><title>Human cord blood</title><author>Tanaskovic, Olga ; Verga Falzacappa, Maria Vittoria ; Pelicci, Pier Giuseppe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g998-4ae44efec0c6a52154c8beb0f226d9922baf2ab150bdc7bcea46fe59a553feb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acute myelocytic leukemia</topic><topic>Bone marrow transplantation</topic><topic>Cancer research</topic><topic>Care and treatment</topic><topic>Development and progression</topic><topic>Health aspects</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Myeloid leukemia</topic><topic>Parenting</topic><topic>Surgery</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanaskovic, Olga</creatorcontrib><creatorcontrib>Verga Falzacappa, Maria Vittoria</creatorcontrib><creatorcontrib>Pelicci, Pier Giuseppe</creatorcontrib><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanaskovic, Olga</au><au>Verga Falzacappa, Maria Vittoria</au><au>Pelicci, Pier Giuseppe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human cord blood</atitle><jtitle>PloS one</jtitle><date>2019-09-19</date><risdate>2019</risdate><volume>14</volume><issue>9</issue><spage>e0217345</spage><pages>e0217345-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Since their appearance, humanized mice carrying human immune system seemed promising tools to study the crosstalk between cancer and immunity. The NOD-scidIL2Rgamma.sup.null (NSG) mice engrafted with human cord blood (hCB)-CD34.sup.+ cells have been proposed to be a valuable tool to reproduce human immune system in mouse. However, the lack of solid evidences on the functionality of their human immune components limits their usage in immune-oncology. We report that (hCB)-CD34.sup.+ cells lose their ability to propagate and originate bone marrow-derived human immune cells after two serial transplantations in NSG mice. We demonstrate that transplants of bone marrow patient-derived acute myeloid leukemias (hAMLs) grow very similarly in the humanized (hCB)-CD34.sup.+ NSG and parental NSG mice. The similar extent of engraftment and development of leukemias in (hCB)-CD34.sup.+ NSG and controls suggests a poor human immune response against not compatible hAMLs. Our findings suggest that (hCB)-CD34.sup.+ NSG mice are transient and/or incomplete carriers of the human immune system and, therefore, represent a suboptimal tool to study the interaction between tumor and immune cells.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0217345</doi><tpages>e0217345</tpages></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2019-09, Vol.14 (9), p.e0217345 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acute myelocytic leukemia Bone marrow transplantation Cancer research Care and treatment Development and progression Health aspects Immune response Immune system Myeloid leukemia Parenting Surgery Tumors |
| title | Human cord blood |
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