Cysteine dioxygenase type 1
Progression of colorectal cancer (CRC) has been explained by genomic abnormalities along with the adenoma-carcinoma sequence theory (ACS). The aim of our study is to elucidate whether the promoter DNA methylation of the cancer-specific methylation gene, cysteine dioxygenase 1 (CDO1), contributes to...
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| Veröffentlicht in: | PloS one 2018-05, Vol.13 (5), p.e0194785 |
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| creator | Kojima, Keita Nakamura, Takatoshi Ohbu, Makoto Katoh, Hiroshi Ooizumi, Yosuke Igarashi, Kazuharu Ishii, Satoru Tanaka, Toshimichi Yokoi, Keigo Nishizawa, Nobuyuki Yokota, Kazuko Kosaka, Yoshimasa Sato, Takeo Watanabe, Masahiko Yamashita, Keishi |
| description | Progression of colorectal cancer (CRC) has been explained by genomic abnormalities along with the adenoma-carcinoma sequence theory (ACS). The aim of our study is to elucidate whether the promoter DNA methylation of the cancer-specific methylation gene, cysteine dioxygenase 1 (CDO1), contributes to the carcinogenic process in CRC. The study group comprised 107 patients with CRC who underwent surgical resection and 90 adenomas treated with endoscopic resection in the Kitasato University Hospital in 2000. We analyzed the extent of methylation in each tissue using quantitative TaqMan methylation-specific PCR for CDO1. The methylation level increased along with the ACS process (p < 0.0001), and statistically significant differences were found between normal-appearing mucosa (NAM) and low-grade adenoma (p < 0.0001), and between low-grade adenoma and high-grade adenoma (p = 0.01), but not between high-grade adenoma and cancer with no liver metastasis. Furthermore, primary CRC cancers with liver metastasis harbored significantly higher methylation of CDO1 than those without liver metastasis (p = 0.02). As a result, the area under the curve by CDO1 promoter methylation was 0.96, 0.80, and 0.67 to discriminate cancer from NAM, low-grade adenoma from NAM, and low-grade adenoma from high-grade adenoma, respectively. CDO1 methylation accumulates during the ACS process, and consistently contributes to CRC progression. |
| doi_str_mv | 10.1371/journal.pone.0194785 |
| format | Article |
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The aim of our study is to elucidate whether the promoter DNA methylation of the cancer-specific methylation gene, cysteine dioxygenase 1 (CDO1), contributes to the carcinogenic process in CRC. The study group comprised 107 patients with CRC who underwent surgical resection and 90 adenomas treated with endoscopic resection in the Kitasato University Hospital in 2000. We analyzed the extent of methylation in each tissue using quantitative TaqMan methylation-specific PCR for CDO1. The methylation level increased along with the ACS process (p < 0.0001), and statistically significant differences were found between normal-appearing mucosa (NAM) and low-grade adenoma (p < 0.0001), and between low-grade adenoma and high-grade adenoma (p = 0.01), but not between high-grade adenoma and cancer with no liver metastasis. Furthermore, primary CRC cancers with liver metastasis harbored significantly higher methylation of CDO1 than those without liver metastasis (p = 0.02). As a result, the area under the curve by CDO1 promoter methylation was 0.96, 0.80, and 0.67 to discriminate cancer from NAM, low-grade adenoma from NAM, and low-grade adenoma from high-grade adenoma, respectively. 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The aim of our study is to elucidate whether the promoter DNA methylation of the cancer-specific methylation gene, cysteine dioxygenase 1 (CDO1), contributes to the carcinogenic process in CRC. The study group comprised 107 patients with CRC who underwent surgical resection and 90 adenomas treated with endoscopic resection in the Kitasato University Hospital in 2000. We analyzed the extent of methylation in each tissue using quantitative TaqMan methylation-specific PCR for CDO1. The methylation level increased along with the ACS process (p < 0.0001), and statistically significant differences were found between normal-appearing mucosa (NAM) and low-grade adenoma (p < 0.0001), and between low-grade adenoma and high-grade adenoma (p = 0.01), but not between high-grade adenoma and cancer with no liver metastasis. Furthermore, primary CRC cancers with liver metastasis harbored significantly higher methylation of CDO1 than those without liver metastasis (p = 0.02). 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CDO1 methylation accumulates during the ACS process, and consistently contributes to CRC progression.</description><subject>Colorectal cancer</subject><subject>Development and progression</subject><subject>DNA</subject><subject>Genetic aspects</subject><subject>Methylation</subject><subject>Promoters (Genetics)</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFzs1Kw0AUBeBBFKzVJ9BFVoKLxLmZzGTusgR_CoWCFrfhtrlJU8KkOBNo3l5BF3Hl6pzFx-EIcQcyAZXD46EfPh11ybF3nEjALLf6TMwAVRqbVKrzSb8UV94fpNTKGjMTt8XoA7eOo6rtT2PDjjxHYTxyBNfioqbO881vzsXm-WlTvMar9cuyWKziBtHErDRUIJEyTWzr3RaIKlNZXcs8symRTRXoDIDRbtGkyqJChpo1InJNai4efmYb6rhs3a53gU-hocH7cvn-Vi6-v8pcSjT_2PXHX3s_sXumLux93w2h7Z2fwi9cIFxf</recordid><startdate>20180510</startdate><enddate>20180510</enddate><creator>Kojima, Keita</creator><creator>Nakamura, Takatoshi</creator><creator>Ohbu, Makoto</creator><creator>Katoh, Hiroshi</creator><creator>Ooizumi, Yosuke</creator><creator>Igarashi, Kazuharu</creator><creator>Ishii, Satoru</creator><creator>Tanaka, Toshimichi</creator><creator>Yokoi, Keigo</creator><creator>Nishizawa, Nobuyuki</creator><creator>Yokota, Kazuko</creator><creator>Kosaka, Yoshimasa</creator><creator>Sato, Takeo</creator><creator>Watanabe, Masahiko</creator><creator>Yamashita, Keishi</creator><general>Public Library of Science</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20180510</creationdate><title>Cysteine dioxygenase type 1</title><author>Kojima, Keita ; Nakamura, Takatoshi ; Ohbu, Makoto ; Katoh, Hiroshi ; Ooizumi, Yosuke ; Igarashi, Kazuharu ; Ishii, Satoru ; Tanaka, Toshimichi ; Yokoi, Keigo ; Nishizawa, Nobuyuki ; Yokota, Kazuko ; Kosaka, Yoshimasa ; Sato, Takeo ; Watanabe, Masahiko ; Yamashita, Keishi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g996-e351d109a45ae8fcb1aad6d85f07482aa82315411e98b96238939e1fe5999efa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Colorectal cancer</topic><topic>Development and progression</topic><topic>DNA</topic><topic>Genetic aspects</topic><topic>Methylation</topic><topic>Promoters (Genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kojima, Keita</creatorcontrib><creatorcontrib>Nakamura, Takatoshi</creatorcontrib><creatorcontrib>Ohbu, Makoto</creatorcontrib><creatorcontrib>Katoh, Hiroshi</creatorcontrib><creatorcontrib>Ooizumi, Yosuke</creatorcontrib><creatorcontrib>Igarashi, Kazuharu</creatorcontrib><creatorcontrib>Ishii, Satoru</creatorcontrib><creatorcontrib>Tanaka, Toshimichi</creatorcontrib><creatorcontrib>Yokoi, Keigo</creatorcontrib><creatorcontrib>Nishizawa, Nobuyuki</creatorcontrib><creatorcontrib>Yokota, Kazuko</creatorcontrib><creatorcontrib>Kosaka, Yoshimasa</creatorcontrib><creatorcontrib>Sato, Takeo</creatorcontrib><creatorcontrib>Watanabe, Masahiko</creatorcontrib><creatorcontrib>Yamashita, Keishi</creatorcontrib><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kojima, Keita</au><au>Nakamura, Takatoshi</au><au>Ohbu, Makoto</au><au>Katoh, Hiroshi</au><au>Ooizumi, Yosuke</au><au>Igarashi, Kazuharu</au><au>Ishii, Satoru</au><au>Tanaka, Toshimichi</au><au>Yokoi, Keigo</au><au>Nishizawa, Nobuyuki</au><au>Yokota, Kazuko</au><au>Kosaka, Yoshimasa</au><au>Sato, Takeo</au><au>Watanabe, Masahiko</au><au>Yamashita, Keishi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cysteine dioxygenase type 1</atitle><jtitle>PloS one</jtitle><date>2018-05-10</date><risdate>2018</risdate><volume>13</volume><issue>5</issue><spage>e0194785</spage><pages>e0194785-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Progression of colorectal cancer (CRC) has been explained by genomic abnormalities along with the adenoma-carcinoma sequence theory (ACS). The aim of our study is to elucidate whether the promoter DNA methylation of the cancer-specific methylation gene, cysteine dioxygenase 1 (CDO1), contributes to the carcinogenic process in CRC. The study group comprised 107 patients with CRC who underwent surgical resection and 90 adenomas treated with endoscopic resection in the Kitasato University Hospital in 2000. We analyzed the extent of methylation in each tissue using quantitative TaqMan methylation-specific PCR for CDO1. The methylation level increased along with the ACS process (p < 0.0001), and statistically significant differences were found between normal-appearing mucosa (NAM) and low-grade adenoma (p < 0.0001), and between low-grade adenoma and high-grade adenoma (p = 0.01), but not between high-grade adenoma and cancer with no liver metastasis. Furthermore, primary CRC cancers with liver metastasis harbored significantly higher methylation of CDO1 than those without liver metastasis (p = 0.02). As a result, the area under the curve by CDO1 promoter methylation was 0.96, 0.80, and 0.67 to discriminate cancer from NAM, low-grade adenoma from NAM, and low-grade adenoma from high-grade adenoma, respectively. CDO1 methylation accumulates during the ACS process, and consistently contributes to CRC progression.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0194785</doi><tpages>e0194785</tpages></addata></record> |
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| subjects | Colorectal cancer Development and progression DNA Genetic aspects Methylation Promoters (Genetics) |
| title | Cysteine dioxygenase type 1 |
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