Analysis of the Enantioselective Effects of PCB95 in Zebrafish
As persistent organic pollutants, polychlorinated biphenyls (PCBs) accumulate in the bodies of animals and humans, resulting in toxic effects on the reproductive, immune, nervous, and endocrine systems. The biological and toxicological characteristics of enantiomers of chiral PCBs may differ, but th...
Gespeichert in:
| Veröffentlicht in: | PloS one 2016-08, Vol.11 (8), p.e0160584 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 8 |
| container_start_page | e0160584 |
| container_title | PloS one |
| container_volume | 11 |
| creator | Xu, Nana Mu, Pengqian Yin, Zhiqiang Jia, Qi Yang, Shuming Qian, Yongzhong Qiu, Jing |
| description | As persistent organic pollutants, polychlorinated biphenyls (PCBs) accumulate in the bodies of animals and humans, resulting in toxic effects on the reproductive, immune, nervous, and endocrine systems. The biological and toxicological characteristics of enantiomers of chiral PCBs may differ, but these enantioselective effects of PCBs have not been fully characterized. In this study, we performed metabolomics analysis, using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the enantioselective toxic effects of PCB95 in zebrafish (Danio rerio) embryos after exposure to three dose levels of 0.1, 1, and 10 [mu]g/L for 72 h. Multivariate analysis directly reflected the metabolic perturbations caused by PCB95. The effects of (-)-PCB95 and (+)-PCB95 were more prominent than those of the racemate in zebrafish embryos. A total of 26 endogenous metabolites were selected as potential marker metabolites with variable importance at projection values larger than 1 and significant differences (p |
| doi_str_mv | 10.1371/journal.pone.0160584 |
| format | Article |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_incontextgauss_ISR_A470631497</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A470631497</galeid><sourcerecordid>A470631497</sourcerecordid><originalsourceid>FETCH-LOGICAL-g997-8b2f24699fcefa2428b53bd3f0e625405d84e0e4c30382ba656d56614cdba3b23</originalsourceid><addsrcrecordid>eNqFkE1Lw0AQhhdRsFb_gYecBA-J-53sRYilaqFQ0eLBS9hNZpOUsJHuRuq_N6iHePI0D_M-M4cXoUuCE8JScrPrh73TXfLeO0gwkVhk_AjNiGI0lhSz4wmfojPvdxgLlkk5Q7f5ePjpWx_1NgoNREunXWh7Dx2Uof0YF9aO9J0_Le6UiFoXvYHZa9v65hydWN15uPidc7S9X24Xj_F687Ba5Ou4ViqNM0Mt5VIpW4LVlNPMCGYqZjFIKjgWVcYBAy8ZZhk1WgpZCSkJLyujmaFsjq5_3ta6g6J1Ze8CHEKtB--L1ctzkfMUS0a4Sv9xN69_3auJ24DuQuP7bhgLcH4qfgGdDmiU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Analysis of the Enantioselective Effects of PCB95 in Zebrafish</title><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Xu, Nana ; Mu, Pengqian ; Yin, Zhiqiang ; Jia, Qi ; Yang, Shuming ; Qian, Yongzhong ; Qiu, Jing</creator><creatorcontrib>Xu, Nana ; Mu, Pengqian ; Yin, Zhiqiang ; Jia, Qi ; Yang, Shuming ; Qian, Yongzhong ; Qiu, Jing</creatorcontrib><description>As persistent organic pollutants, polychlorinated biphenyls (PCBs) accumulate in the bodies of animals and humans, resulting in toxic effects on the reproductive, immune, nervous, and endocrine systems. The biological and toxicological characteristics of enantiomers of chiral PCBs may differ, but these enantioselective effects of PCBs have not been fully characterized. In this study, we performed metabolomics analysis, using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the enantioselective toxic effects of PCB95 in zebrafish (Danio rerio) embryos after exposure to three dose levels of 0.1, 1, and 10 [mu]g/L for 72 h. Multivariate analysis directly reflected the metabolic perturbations caused by PCB95. The effects of (-)-PCB95 and (+)-PCB95 were more prominent than those of the racemate in zebrafish embryos. A total of 26 endogenous metabolites were selected as potential marker metabolites with variable importance at projection values larger than 1 and significant differences (p<0.05). These metabolites included amino acids, organic acids, nucleosides, betaine, and choline. The changes in these biomarkers were dependent on the enantiomer-specific structures of PCB95. Fifteen metabolic pathways were significantly affected, and several nervous and immune system-related metabolites were significantly validated after exposure. These metabolic changes indicated that the toxic effects of PCB95 may be associated with the interaction of PCB95 with the nervous and immune systems, thus resulting in disruption of energy metabolism and liver function.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0160584</identifier><language>eng</language><publisher>Public Library of Science</publisher><subject>Amino acids ; Analysis ; Chlorinated solvents ; Embryonic development ; Enantiomers ; High performance liquid chromatography ; Mass spectrometry ; Metabolites ; Persistent organic pollutants ; Polychlorinated biphenyls</subject><ispartof>PloS one, 2016-08, Vol.11 (8), p.e0160584</ispartof><rights>COPYRIGHT 2016 Public Library of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Xu, Nana</creatorcontrib><creatorcontrib>Mu, Pengqian</creatorcontrib><creatorcontrib>Yin, Zhiqiang</creatorcontrib><creatorcontrib>Jia, Qi</creatorcontrib><creatorcontrib>Yang, Shuming</creatorcontrib><creatorcontrib>Qian, Yongzhong</creatorcontrib><creatorcontrib>Qiu, Jing</creatorcontrib><title>Analysis of the Enantioselective Effects of PCB95 in Zebrafish</title><title>PloS one</title><description>As persistent organic pollutants, polychlorinated biphenyls (PCBs) accumulate in the bodies of animals and humans, resulting in toxic effects on the reproductive, immune, nervous, and endocrine systems. The biological and toxicological characteristics of enantiomers of chiral PCBs may differ, but these enantioselective effects of PCBs have not been fully characterized. In this study, we performed metabolomics analysis, using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the enantioselective toxic effects of PCB95 in zebrafish (Danio rerio) embryos after exposure to three dose levels of 0.1, 1, and 10 [mu]g/L for 72 h. Multivariate analysis directly reflected the metabolic perturbations caused by PCB95. The effects of (-)-PCB95 and (+)-PCB95 were more prominent than those of the racemate in zebrafish embryos. A total of 26 endogenous metabolites were selected as potential marker metabolites with variable importance at projection values larger than 1 and significant differences (p<0.05). These metabolites included amino acids, organic acids, nucleosides, betaine, and choline. The changes in these biomarkers were dependent on the enantiomer-specific structures of PCB95. Fifteen metabolic pathways were significantly affected, and several nervous and immune system-related metabolites were significantly validated after exposure. These metabolic changes indicated that the toxic effects of PCB95 may be associated with the interaction of PCB95 with the nervous and immune systems, thus resulting in disruption of energy metabolism and liver function.</description><subject>Amino acids</subject><subject>Analysis</subject><subject>Chlorinated solvents</subject><subject>Embryonic development</subject><subject>Enantiomers</subject><subject>High performance liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Metabolites</subject><subject>Persistent organic pollutants</subject><subject>Polychlorinated biphenyls</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqFkE1Lw0AQhhdRsFb_gYecBA-J-53sRYilaqFQ0eLBS9hNZpOUsJHuRuq_N6iHePI0D_M-M4cXoUuCE8JScrPrh73TXfLeO0gwkVhk_AjNiGI0lhSz4wmfojPvdxgLlkk5Q7f5ePjpWx_1NgoNREunXWh7Dx2Uof0YF9aO9J0_Le6UiFoXvYHZa9v65hydWN15uPidc7S9X24Xj_F687Ba5Ou4ViqNM0Mt5VIpW4LVlNPMCGYqZjFIKjgWVcYBAy8ZZhk1WgpZCSkJLyujmaFsjq5_3ta6g6J1Ze8CHEKtB--L1ctzkfMUS0a4Sv9xN69_3auJ24DuQuP7bhgLcH4qfgGdDmiU</recordid><startdate>20160808</startdate><enddate>20160808</enddate><creator>Xu, Nana</creator><creator>Mu, Pengqian</creator><creator>Yin, Zhiqiang</creator><creator>Jia, Qi</creator><creator>Yang, Shuming</creator><creator>Qian, Yongzhong</creator><creator>Qiu, Jing</creator><general>Public Library of Science</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20160808</creationdate><title>Analysis of the Enantioselective Effects of PCB95 in Zebrafish</title><author>Xu, Nana ; Mu, Pengqian ; Yin, Zhiqiang ; Jia, Qi ; Yang, Shuming ; Qian, Yongzhong ; Qiu, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g997-8b2f24699fcefa2428b53bd3f0e625405d84e0e4c30382ba656d56614cdba3b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amino acids</topic><topic>Analysis</topic><topic>Chlorinated solvents</topic><topic>Embryonic development</topic><topic>Enantiomers</topic><topic>High performance liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Metabolites</topic><topic>Persistent organic pollutants</topic><topic>Polychlorinated biphenyls</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Nana</creatorcontrib><creatorcontrib>Mu, Pengqian</creatorcontrib><creatorcontrib>Yin, Zhiqiang</creatorcontrib><creatorcontrib>Jia, Qi</creatorcontrib><creatorcontrib>Yang, Shuming</creatorcontrib><creatorcontrib>Qian, Yongzhong</creatorcontrib><creatorcontrib>Qiu, Jing</creatorcontrib><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Nana</au><au>Mu, Pengqian</au><au>Yin, Zhiqiang</au><au>Jia, Qi</au><au>Yang, Shuming</au><au>Qian, Yongzhong</au><au>Qiu, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the Enantioselective Effects of PCB95 in Zebrafish</atitle><jtitle>PloS one</jtitle><date>2016-08-08</date><risdate>2016</risdate><volume>11</volume><issue>8</issue><spage>e0160584</spage><pages>e0160584-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>As persistent organic pollutants, polychlorinated biphenyls (PCBs) accumulate in the bodies of animals and humans, resulting in toxic effects on the reproductive, immune, nervous, and endocrine systems. The biological and toxicological characteristics of enantiomers of chiral PCBs may differ, but these enantioselective effects of PCBs have not been fully characterized. In this study, we performed metabolomics analysis, using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to investigate the enantioselective toxic effects of PCB95 in zebrafish (Danio rerio) embryos after exposure to three dose levels of 0.1, 1, and 10 [mu]g/L for 72 h. Multivariate analysis directly reflected the metabolic perturbations caused by PCB95. The effects of (-)-PCB95 and (+)-PCB95 were more prominent than those of the racemate in zebrafish embryos. A total of 26 endogenous metabolites were selected as potential marker metabolites with variable importance at projection values larger than 1 and significant differences (p<0.05). These metabolites included amino acids, organic acids, nucleosides, betaine, and choline. The changes in these biomarkers were dependent on the enantiomer-specific structures of PCB95. Fifteen metabolic pathways were significantly affected, and several nervous and immune system-related metabolites were significantly validated after exposure. These metabolic changes indicated that the toxic effects of PCB95 may be associated with the interaction of PCB95 with the nervous and immune systems, thus resulting in disruption of energy metabolism and liver function.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0160584</doi><tpages>e0160584</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2016-08, Vol.11 (8), p.e0160584 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_gale_incontextgauss_ISR_A470631497 |
| source | DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Analysis Chlorinated solvents Embryonic development Enantiomers High performance liquid chromatography Mass spectrometry Metabolites Persistent organic pollutants Polychlorinated biphenyls |
| title | Analysis of the Enantioselective Effects of PCB95 in Zebrafish |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T13%3A02%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20the%20Enantioselective%20Effects%20of%20PCB95%20in%20Zebrafish&rft.jtitle=PloS%20one&rft.au=Xu,%20Nana&rft.date=2016-08-08&rft.volume=11&rft.issue=8&rft.spage=e0160584&rft.pages=e0160584-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0160584&rft_dat=%3Cgale%3EA470631497%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A470631497&rfr_iscdi=true |