Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon
Improved methods for genetically sexing salmonids and for characterization of Y-chromosome homologies between species can contribute to understanding the evolution of sex chromosomes and sex-determining mechanisms. In this study we have explored 12.5 kb of Y-chromosome-specific sequence flanking the...
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| Veröffentlicht in: | Genome 2008-09, Vol.51 (9), p.739-748 |
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| creator | Brunelli, Joseph P Wertzler, Kelsey J Sundin, Kyle Thorgaard, Gary H |
| description | Improved methods for genetically sexing salmonids and for characterization of Y-chromosome homologies between species can contribute to understanding the evolution of sex chromosomes and sex-determining mechanisms. In this study we have explored 12.5 kb of Y-chromosome-specific sequence flanking the previously described OtY2 locus in Chinook salmon (
Oncorhynchus tshawytscha
) and 21 kb of homologous rainbow trout (
Oncorhynchus mykiss
) Y-chromosome-specific sequence. This is the first confirmed Y-specific sequence for rainbow trout. New Y-specific markers are described for Chinook salmon (OtY3) and rainbow trout (OmyY1), which are readily detected by PCR assays and are advantageous because they also produce autosomal control amplification products. Additionally, AFLP analysis of Chinook salmon yielded another potential Y-chromosome marker. These descriptions will facilitate genotypic sexing and should be useful for population studies of Y-chromosome polymorphisms and for future studies to characterize what appears to be a common sex-determining mechanism between these species. |
| doi_str_mv | 10.1139/G08-060 |
| format | Article |
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Oncorhynchus tshawytscha
) and 21 kb of homologous rainbow trout (
Oncorhynchus mykiss
) Y-chromosome-specific sequence. This is the first confirmed Y-specific sequence for rainbow trout. New Y-specific markers are described for Chinook salmon (OtY3) and rainbow trout (OmyY1), which are readily detected by PCR assays and are advantageous because they also produce autosomal control amplification products. Additionally, AFLP analysis of Chinook salmon yielded another potential Y-chromosome marker. These descriptions will facilitate genotypic sexing and should be useful for population studies of Y-chromosome polymorphisms and for future studies to characterize what appears to be a common sex-determining mechanism between these species.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/G08-060</identifier><identifier>PMID: 18772952</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Canada: NRC Research Press</publisher><subject>Animals ; Base Sequence ; Brackish ; Chinook salmon ; chromosome sexuel ; chromosome Y ; Chromosomes ; Cloning, Molecular ; DNA - genetics ; DNA Primers ; Female ; Freshwater ; Genetic aspects ; Genetic markers ; Genetic polymorphisms ; Identification and classification ; Male ; Marine ; Molecular Sequence Data ; Oncorhynchus ; Oncorhynchus mykiss ; Oncorhynchus mykiss - genetics ; Oncorhynchus tshawytscha ; Polymerase Chain Reaction ; Polymorphism ; Polymorphism, Genetic ; polymorphisme du chromosome Y ; Population studies ; Rainbow trout ; Retroelements ; Salmon ; Salmon - genetics ; saumon ; sex chromosome ; Studies ; Trout ; truite ; Y Chromosome ; Y chromosome polymorphism</subject><ispartof>Genome, 2008-09, Vol.51 (9), p.739-748</ispartof><rights>COPYRIGHT 2008 NRC Research Press</rights><rights>Copyright National Research Council of Canada Sep 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c641t-c803ff2c296c3fe5d918c1db771926dc6917092681cd8f0e951e4296130bf2fb3</citedby><cites>FETCH-LOGICAL-c641t-c803ff2c296c3fe5d918c1db771926dc6917092681cd8f0e951e4296130bf2fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/G08-060$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/G08-060$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>315,781,785,2933,27926,27927,64430,65236</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18772952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Danzmann, Roy</contributor><creatorcontrib>Brunelli, Joseph P</creatorcontrib><creatorcontrib>Wertzler, Kelsey J</creatorcontrib><creatorcontrib>Sundin, Kyle</creatorcontrib><creatorcontrib>Thorgaard, Gary H</creatorcontrib><title>Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon</title><title>Genome</title><addtitle>Génome</addtitle><description>Improved methods for genetically sexing salmonids and for characterization of Y-chromosome homologies between species can contribute to understanding the evolution of sex chromosomes and sex-determining mechanisms. In this study we have explored 12.5 kb of Y-chromosome-specific sequence flanking the previously described OtY2 locus in Chinook salmon (
Oncorhynchus tshawytscha
) and 21 kb of homologous rainbow trout (
Oncorhynchus mykiss
) Y-chromosome-specific sequence. This is the first confirmed Y-specific sequence for rainbow trout. New Y-specific markers are described for Chinook salmon (OtY3) and rainbow trout (OmyY1), which are readily detected by PCR assays and are advantageous because they also produce autosomal control amplification products. Additionally, AFLP analysis of Chinook salmon yielded another potential Y-chromosome marker. These descriptions will facilitate genotypic sexing and should be useful for population studies of Y-chromosome polymorphisms and for future studies to characterize what appears to be a common sex-determining mechanism between these species.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Brackish</subject><subject>Chinook salmon</subject><subject>chromosome sexuel</subject><subject>chromosome Y</subject><subject>Chromosomes</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Freshwater</subject><subject>Genetic aspects</subject><subject>Genetic markers</subject><subject>Genetic polymorphisms</subject><subject>Identification and classification</subject><subject>Male</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Oncorhynchus</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>Oncorhynchus tshawytscha</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Genetic</subject><subject>polymorphisme du chromosome Y</subject><subject>Population studies</subject><subject>Rainbow trout</subject><subject>Retroelements</subject><subject>Salmon</subject><subject>Salmon - genetics</subject><subject>saumon</subject><subject>sex chromosome</subject><subject>Studies</subject><subject>Trout</subject><subject>truite</subject><subject>Y Chromosome</subject><subject>Y chromosome polymorphism</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqV0l1rFDEUBuAgil2r-A9k8EJRmJqT7Mwkl2XRWikKflx4FTKZk93UmWSazKD990Z3sd1SEclFQnjyJjkcQh4DPQLg8tUJFSWt6R2ygKWgJecM7pIFFRxK1sj6gDxI6ZxSoFzCfXIAommYrNiCvPtaphGNs84UCS9m9AZToX1XjKG_HEIcNy4NqXC-iNr5Nnwvphjm6TdZbZwP4VuRdD8E_5Dcs7pP-Gg3H5Ivb15_Xr0tzz6cnK6Oz0pTL2EqjaDcWmaYrA23WHUShIGubRqQrO5MLaGheSXAdMJSlBXgMmPgtLXMtvyQPNvmjjHkB6dJDS4Z7HvtMcxJ1flEJVn1TwiSA4hKZvj0BjwPc_T5E4oxuqzrBkRG5RatdY_KeRumqM0aPUbdB4_W5e3jnLfkVNJroXvejO5CXUdHt6A8OhycuTX1xd6BbCb8Ma31nJI6_fTxP-z7fft8a00MKUW0aoxu0PFSAVW_WkzlFlO5xbJ8sivW3A7YXbldT12V3UcTMaGOZvNHrbcpauxshi__Dm9e-xOOU-E6</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Brunelli, Joseph P</creator><creator>Wertzler, Kelsey J</creator><creator>Sundin, Kyle</creator><creator>Thorgaard, Gary H</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISN</scope><scope>ISR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon</title><author>Brunelli, Joseph P ; Wertzler, Kelsey J ; Sundin, Kyle ; Thorgaard, Gary H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c641t-c803ff2c296c3fe5d918c1db771926dc6917092681cd8f0e951e4296130bf2fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Brackish</topic><topic>Chinook salmon</topic><topic>chromosome sexuel</topic><topic>chromosome Y</topic><topic>Chromosomes</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Freshwater</topic><topic>Genetic aspects</topic><topic>Genetic markers</topic><topic>Genetic polymorphisms</topic><topic>Identification and classification</topic><topic>Male</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Oncorhynchus</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>Oncorhynchus tshawytscha</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Genetic</topic><topic>polymorphisme du chromosome Y</topic><topic>Population studies</topic><topic>Rainbow trout</topic><topic>Retroelements</topic><topic>Salmon</topic><topic>Salmon - genetics</topic><topic>saumon</topic><topic>sex chromosome</topic><topic>Studies</topic><topic>Trout</topic><topic>truite</topic><topic>Y Chromosome</topic><topic>Y chromosome polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brunelli, Joseph P</creatorcontrib><creatorcontrib>Wertzler, Kelsey J</creatorcontrib><creatorcontrib>Sundin, Kyle</creatorcontrib><creatorcontrib>Thorgaard, Gary H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Canada</collection><collection>Gale In Context: Science</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brunelli, Joseph P</au><au>Wertzler, Kelsey J</au><au>Sundin, Kyle</au><au>Thorgaard, Gary H</au><au>Danzmann, Roy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>51</volume><issue>9</issue><spage>739</spage><epage>748</epage><pages>739-748</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>Improved methods for genetically sexing salmonids and for characterization of Y-chromosome homologies between species can contribute to understanding the evolution of sex chromosomes and sex-determining mechanisms. In this study we have explored 12.5 kb of Y-chromosome-specific sequence flanking the previously described OtY2 locus in Chinook salmon (
Oncorhynchus tshawytscha
) and 21 kb of homologous rainbow trout (
Oncorhynchus mykiss
) Y-chromosome-specific sequence. This is the first confirmed Y-specific sequence for rainbow trout. New Y-specific markers are described for Chinook salmon (OtY3) and rainbow trout (OmyY1), which are readily detected by PCR assays and are advantageous because they also produce autosomal control amplification products. Additionally, AFLP analysis of Chinook salmon yielded another potential Y-chromosome marker. These descriptions will facilitate genotypic sexing and should be useful for population studies of Y-chromosome polymorphisms and for future studies to characterize what appears to be a common sex-determining mechanism between these species.</abstract><cop>Canada</cop><pub>NRC Research Press</pub><pmid>18772952</pmid><doi>10.1139/G08-060</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Base Sequence Brackish Chinook salmon chromosome sexuel chromosome Y Chromosomes Cloning, Molecular DNA - genetics DNA Primers Female Freshwater Genetic aspects Genetic markers Genetic polymorphisms Identification and classification Male Marine Molecular Sequence Data Oncorhynchus Oncorhynchus mykiss Oncorhynchus mykiss - genetics Oncorhynchus tshawytscha Polymerase Chain Reaction Polymorphism Polymorphism, Genetic polymorphisme du chromosome Y Population studies Rainbow trout Retroelements Salmon Salmon - genetics saumon sex chromosome Studies Trout truite Y Chromosome Y chromosome polymorphism |
| title | Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon |
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