Detection of drug resistant Mycobacterium tuberculosis by high-throughput sequencing of DNA isolated from acid fast bacilli smears

Drug susceptibility testing for Mycobacterium tuberculosis (MTB) is difficult to perform in resource-limited settings where Acid Fast Bacilli (AFB) smears are commonly used for disease diagnosis and monitoring. We developed a simple method for extraction of MTB DNA from AFB smears for sequencing-bas...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PLoS ONE 2020, Vol.15 (5), p.e0232343
Hauptverfasser:	Rowneki, Mazhgan, Aronson, Naomi, Du, Peicheng, Sachs, Paige, Blakemore, Robert, Chakravorty, Soumitesh, Levy, Shawn, Jones, Angela L, Trivedi, Geetika, Chebore, Sheilla, Addo, Dennis, Byarugaba, Denis K, Njobvu, Panganani Dalisani, Wabwire-Mangen, Frederick, Erima, Bernard, Ramos, Eric S, Evans, Carlton A, Hale, Braden, Mancuso, James D, Alland, David
Format:	Report
Sprache:	eng
Schlagworte:	Air bases Antitubercular agents Diagnosis Disease susceptibility Diseases DNA DNA sequencing Drug resistance Drugs Genes Genetic aspects Genetic research Methods Microbial drug resistance Molecular diagnostic techniques Mycobacterium tuberculosis Physiological aspects Setting (Literature) Tuberculosis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!