Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow (Ay/a) Obese Mice
Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow ( A y /a ) Obese Mice Takayuki Masaki 1 , Seiichi Chiba 1 , Tohru Yasuda 1 , Tetsuo Tsubone 1 , Tetsuya Kakuma 1 , Iichiro Shimomura 2 , Tohru F...
Gespeichert in:
| Veröffentlicht in: | Diabetes (New York, N.Y.) N.Y.), 2003-09, Vol.52 (9), p.2266-2273 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2273 |
|---|---|
| container_issue | 9 |
| container_start_page | 2266 |
| container_title | Diabetes (New York, N.Y.) |
| container_volume | 52 |
| creator | MASAKI, Takayuki CHIBA, Seiichi YASUDA, Tohru TSUBONE, Tetsuo KAKUMA, Tetsuya SHIMOMURA, Iichiro FUNAHASHI, Tohru MATSUZAWA, Yuji YOSHIMATSU, Hironobu |
| description | Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling
Protein in Agouti Yellow ( A y /a ) Obese Mice
Takayuki Masaki 1 ,
Seiichi Chiba 1 ,
Tohru Yasuda 1 ,
Tetsuo Tsubone 1 ,
Tetsuya Kakuma 1 ,
Iichiro Shimomura 2 ,
Tohru Funahashi 3 ,
Yuji Matsuzawa 3 and
Hironobu Yoshimatsu 1
1 Department of Internal Medicine, School of Medicine, Oita Medical University, Oita, Japan
2 Department of Medicine and Pathophysiology, Graduate School of Medicine, Osaka University, Osaka, Japan
3 Department of Internal Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
Address correspondence and reprint requests to Dr. Takayuki Masaki, Department of Internal Medicine, School of Medicine, Oita
Medical University, Hasama, Oita, 879-5593, Japan. E-mail: masaki{at}oita-med.ac.jp
Abstract
To examine the peripheral and central roles of adiponectin in energy intake and expenditure, we investigated the effects of
adiponectin on food intake, adiposity, sympathetic nerve activity (SNA), and mRNA expressions of uncoupling protein (UCP)
in the brown adipose tissue (BAT), white adipose tissue (WAT) and skeletal muscle in agouti yellow ( A y /a ) obese mice. Intraperitoneal administration of adiponectin (1.5 mg/kg for 7 days) attenuated body weight gain and reduced
visceral adiposity in A y /a obese mice compared with PBS-treated controls. In addition, adiponectin treatment increased the expression of UCP1 mRNA in
BAT, UCP2 mRNA in WAT, and UCP3 mRNA in skeletal muscle compared with PBS-treated A y /a controls. Acute peripheral administration of adiponectin (1.5 mg/kg, one injection) also increased SNA in the BAT accompanied
by an increase in rectal temperature. Finally, these above responses as well as expression of c-Fos–like immunohistochemistry
in the hypothalamus were not induced by central application of adiponectin (0–15 μg/kg). Taken together, adiponectin effectively
regulated visceral adiposity, SNA, and UCP mRNA expression peripherally, suggesting that this substance can be used as a therapeutic
tool, administered peripherally, in the treatment of visceral obesity and related metabolic disorders.
ARC, arcuate nucleus
BAT, brown adipose tissue
ICV, intracerebroventricular
PVN, paraventricular nucleus
SNA, sympathetic nerve activity
UCP, uncoupling protein
VMH, ventromedial hypothalamus
WAT, white adipose tissue
Footnotes
Accepted June 18, 2003.
Received March 18, 2003.
DIABETES |
| doi_str_mv | 10.2337/diabetes.52.9.2266 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_highw</sourceid><recordid>TN_cdi_gale_incontextcollege_GICCO_A107754838</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A107754838</galeid><sourcerecordid>A107754838</sourcerecordid><originalsourceid>FETCH-LOGICAL-c585t-a45840ae8ebfd38c631bd5bc93abaca4772cd0704991204be1275cdcb778fc723</originalsourceid><addsrcrecordid>eNptkt2KEzEUxwdR3Lr6Al5IEBQXnW4-ZprJZS3rKlS7iBW9CpnMmWmWaTJOMuz2oXxHU1splXJCQk5-5yN_TpI8J3hMGeOXlVElBPDjnI7FmNLJ5EEyIoKJlFH-42EywpjQlHDBz5In3t9ijCfRHidnhIqM8Ek-Sn7fQG-6FfSqfYfeDwF9cQHNwIa_jmm1Ntb4eAnGWeTq6DGds6CDsegrVIMGj74br7cJdo_ehA1StkLLrodmaFXsEIUVoKv76PB-n2hptRu61tgG3fQuQMwX17RxQzDoJ7Stu0NvpptLdYEWJXhAn42Gp8mjWrUenu3P82T54erb7GM6X1x_mk3nqc6LPKQqy4sMKyigrCtW6AkjZZWXWjBVKq0yzqmuMMeZEITirARCea4rXXJe1JpTdp683uXtevdrAB_kevvHtlUW3OAlZ7lgXLAIvvwPvHVDb2NvkpJJVsQNRyjdQY1qQRpbuyiobsBuRYti1ia6pwRznmcFKyI_PsFHq2Bt9MmAi6OAyAS4D40avJfF9fyYTU-x2rUtNCCjjLPFMU93vO6d9z3UsuvNWvUbSbDcjqH8N4Yyp1LI7RjGoBd7WYZyDdUhZD93EXi1B5TXqq17ZbXxBy7HgtM8i9zbHbcyzerO9HCodqLsHwd29lE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>216482160</pqid></control><display><type>article</type><title>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow (Ay/a) Obese Mice</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>MASAKI, Takayuki ; CHIBA, Seiichi ; YASUDA, Tohru ; TSUBONE, Tetsuo ; KAKUMA, Tetsuya ; SHIMOMURA, Iichiro ; FUNAHASHI, Tohru ; MATSUZAWA, Yuji ; YOSHIMATSU, Hironobu</creator><creatorcontrib>MASAKI, Takayuki ; CHIBA, Seiichi ; YASUDA, Tohru ; TSUBONE, Tetsuo ; KAKUMA, Tetsuya ; SHIMOMURA, Iichiro ; FUNAHASHI, Tohru ; MATSUZAWA, Yuji ; YOSHIMATSU, Hironobu</creatorcontrib><description>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling
Protein in Agouti Yellow ( A y /a ) Obese Mice
Takayuki Masaki 1 ,
Seiichi Chiba 1 ,
Tohru Yasuda 1 ,
Tetsuo Tsubone 1 ,
Tetsuya Kakuma 1 ,
Iichiro Shimomura 2 ,
Tohru Funahashi 3 ,
Yuji Matsuzawa 3 and
Hironobu Yoshimatsu 1
1 Department of Internal Medicine, School of Medicine, Oita Medical University, Oita, Japan
2 Department of Medicine and Pathophysiology, Graduate School of Medicine, Osaka University, Osaka, Japan
3 Department of Internal Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
Address correspondence and reprint requests to Dr. Takayuki Masaki, Department of Internal Medicine, School of Medicine, Oita
Medical University, Hasama, Oita, 879-5593, Japan. E-mail: masaki{at}oita-med.ac.jp
Abstract
To examine the peripheral and central roles of adiponectin in energy intake and expenditure, we investigated the effects of
adiponectin on food intake, adiposity, sympathetic nerve activity (SNA), and mRNA expressions of uncoupling protein (UCP)
in the brown adipose tissue (BAT), white adipose tissue (WAT) and skeletal muscle in agouti yellow ( A y /a ) obese mice. Intraperitoneal administration of adiponectin (1.5 mg/kg for 7 days) attenuated body weight gain and reduced
visceral adiposity in A y /a obese mice compared with PBS-treated controls. In addition, adiponectin treatment increased the expression of UCP1 mRNA in
BAT, UCP2 mRNA in WAT, and UCP3 mRNA in skeletal muscle compared with PBS-treated A y /a controls. Acute peripheral administration of adiponectin (1.5 mg/kg, one injection) also increased SNA in the BAT accompanied
by an increase in rectal temperature. Finally, these above responses as well as expression of c-Fos–like immunohistochemistry
in the hypothalamus were not induced by central application of adiponectin (0–15 μg/kg). Taken together, adiponectin effectively
regulated visceral adiposity, SNA, and UCP mRNA expression peripherally, suggesting that this substance can be used as a therapeutic
tool, administered peripherally, in the treatment of visceral obesity and related metabolic disorders.
ARC, arcuate nucleus
BAT, brown adipose tissue
ICV, intracerebroventricular
PVN, paraventricular nucleus
SNA, sympathetic nerve activity
UCP, uncoupling protein
VMH, ventromedial hypothalamus
WAT, white adipose tissue
Footnotes
Accepted June 18, 2003.
Received March 18, 2003.
DIABETES</description><identifier>ISSN: 0012-1797</identifier><identifier>EISSN: 1939-327X</identifier><identifier>DOI: 10.2337/diabetes.52.9.2266</identifier><identifier>PMID: 12941765</identifier><identifier>CODEN: DIAEAZ</identifier><language>eng</language><publisher>Alexandria, VA: American Diabetes Association</publisher><subject>Adiponectin ; Adipose tissue ; Adipose Tissue, Brown - drug effects ; Adipose tissues ; Animals ; Biological and medical sciences ; Blood Glucose - drug effects ; Body Temperature - drug effects ; Body Weight - drug effects ; Carrier Proteins - genetics ; Diabetes ; Diabetes mellitus ; Diabetes. Impaired glucose tolerance ; Eating - drug effects ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Gene Expression - drug effects ; Hypothalamus - drug effects ; Hypothalamus - metabolism ; Ingestion ; Injections, Intraperitoneal ; Injections, Intraventricular ; Insulin - blood ; Intercellular Signaling Peptides and Proteins ; Ion Channels ; Liver - drug effects ; Male ; Medical sciences ; Membrane Proteins - genetics ; Membrane Transport Proteins ; Metabolic diseases ; Mice ; Mice, Obese ; Mitochondrial Proteins ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - physiology ; Obesity ; Obesity - drug therapy ; Obesity - physiopathology ; Physiological aspects ; Proteins - genetics ; Proteins - pharmacology ; Proto-Oncogene Proteins c-fos - metabolism ; RNA, Messenger - analysis ; Triglycerides - blood ; Uncoupling Protein 1 ; Uncoupling Protein 2 ; Up-Regulation - drug effects ; Viscera</subject><ispartof>Diabetes (New York, N.Y.), 2003-09, Vol.52 (9), p.2266-2273</ispartof><rights>2004 INIST-CNRS</rights><rights>COPYRIGHT 2003 American Diabetes Association</rights><rights>COPYRIGHT 2003 American Diabetes Association</rights><rights>Copyright American Diabetes Association Sep 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c585t-a45840ae8ebfd38c631bd5bc93abaca4772cd0704991204be1275cdcb778fc723</citedby><cites>FETCH-LOGICAL-c585t-a45840ae8ebfd38c631bd5bc93abaca4772cd0704991204be1275cdcb778fc723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15097254$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12941765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MASAKI, Takayuki</creatorcontrib><creatorcontrib>CHIBA, Seiichi</creatorcontrib><creatorcontrib>YASUDA, Tohru</creatorcontrib><creatorcontrib>TSUBONE, Tetsuo</creatorcontrib><creatorcontrib>KAKUMA, Tetsuya</creatorcontrib><creatorcontrib>SHIMOMURA, Iichiro</creatorcontrib><creatorcontrib>FUNAHASHI, Tohru</creatorcontrib><creatorcontrib>MATSUZAWA, Yuji</creatorcontrib><creatorcontrib>YOSHIMATSU, Hironobu</creatorcontrib><title>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow (Ay/a) Obese Mice</title><title>Diabetes (New York, N.Y.)</title><addtitle>Diabetes</addtitle><description>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling
Protein in Agouti Yellow ( A y /a ) Obese Mice
Takayuki Masaki 1 ,
Seiichi Chiba 1 ,
Tohru Yasuda 1 ,
Tetsuo Tsubone 1 ,
Tetsuya Kakuma 1 ,
Iichiro Shimomura 2 ,
Tohru Funahashi 3 ,
Yuji Matsuzawa 3 and
Hironobu Yoshimatsu 1
1 Department of Internal Medicine, School of Medicine, Oita Medical University, Oita, Japan
2 Department of Medicine and Pathophysiology, Graduate School of Medicine, Osaka University, Osaka, Japan
3 Department of Internal Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
Address correspondence and reprint requests to Dr. Takayuki Masaki, Department of Internal Medicine, School of Medicine, Oita
Medical University, Hasama, Oita, 879-5593, Japan. E-mail: masaki{at}oita-med.ac.jp
Abstract
To examine the peripheral and central roles of adiponectin in energy intake and expenditure, we investigated the effects of
adiponectin on food intake, adiposity, sympathetic nerve activity (SNA), and mRNA expressions of uncoupling protein (UCP)
in the brown adipose tissue (BAT), white adipose tissue (WAT) and skeletal muscle in agouti yellow ( A y /a ) obese mice. Intraperitoneal administration of adiponectin (1.5 mg/kg for 7 days) attenuated body weight gain and reduced
visceral adiposity in A y /a obese mice compared with PBS-treated controls. In addition, adiponectin treatment increased the expression of UCP1 mRNA in
BAT, UCP2 mRNA in WAT, and UCP3 mRNA in skeletal muscle compared with PBS-treated A y /a controls. Acute peripheral administration of adiponectin (1.5 mg/kg, one injection) also increased SNA in the BAT accompanied
by an increase in rectal temperature. Finally, these above responses as well as expression of c-Fos–like immunohistochemistry
in the hypothalamus were not induced by central application of adiponectin (0–15 μg/kg). Taken together, adiponectin effectively
regulated visceral adiposity, SNA, and UCP mRNA expression peripherally, suggesting that this substance can be used as a therapeutic
tool, administered peripherally, in the treatment of visceral obesity and related metabolic disorders.
ARC, arcuate nucleus
BAT, brown adipose tissue
ICV, intracerebroventricular
PVN, paraventricular nucleus
SNA, sympathetic nerve activity
UCP, uncoupling protein
VMH, ventromedial hypothalamus
WAT, white adipose tissue
Footnotes
Accepted June 18, 2003.
Received March 18, 2003.
DIABETES</description><subject>Adiponectin</subject><subject>Adipose tissue</subject><subject>Adipose Tissue, Brown - drug effects</subject><subject>Adipose tissues</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - drug effects</subject><subject>Body Temperature - drug effects</subject><subject>Body Weight - drug effects</subject><subject>Carrier Proteins - genetics</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Eating - drug effects</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Gene Expression - drug effects</subject><subject>Hypothalamus - drug effects</subject><subject>Hypothalamus - metabolism</subject><subject>Ingestion</subject><subject>Injections, Intraperitoneal</subject><subject>Injections, Intraventricular</subject><subject>Insulin - blood</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Ion Channels</subject><subject>Liver - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Transport Proteins</subject><subject>Metabolic diseases</subject><subject>Mice</subject><subject>Mice, Obese</subject><subject>Mitochondrial Proteins</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - physiology</subject><subject>Obesity</subject><subject>Obesity - drug therapy</subject><subject>Obesity - physiopathology</subject><subject>Physiological aspects</subject><subject>Proteins - genetics</subject><subject>Proteins - pharmacology</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>Triglycerides - blood</subject><subject>Uncoupling Protein 1</subject><subject>Uncoupling Protein 2</subject><subject>Up-Regulation - drug effects</subject><subject>Viscera</subject><issn>0012-1797</issn><issn>1939-327X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkt2KEzEUxwdR3Lr6Al5IEBQXnW4-ZprJZS3rKlS7iBW9CpnMmWmWaTJOMuz2oXxHU1splXJCQk5-5yN_TpI8J3hMGeOXlVElBPDjnI7FmNLJ5EEyIoKJlFH-42EywpjQlHDBz5In3t9ijCfRHidnhIqM8Ek-Sn7fQG-6FfSqfYfeDwF9cQHNwIa_jmm1Ntb4eAnGWeTq6DGds6CDsegrVIMGj74br7cJdo_ehA1StkLLrodmaFXsEIUVoKv76PB-n2hptRu61tgG3fQuQMwX17RxQzDoJ7Stu0NvpptLdYEWJXhAn42Gp8mjWrUenu3P82T54erb7GM6X1x_mk3nqc6LPKQqy4sMKyigrCtW6AkjZZWXWjBVKq0yzqmuMMeZEITirARCea4rXXJe1JpTdp683uXtevdrAB_kevvHtlUW3OAlZ7lgXLAIvvwPvHVDb2NvkpJJVsQNRyjdQY1qQRpbuyiobsBuRYti1ia6pwRznmcFKyI_PsFHq2Bt9MmAi6OAyAS4D40avJfF9fyYTU-x2rUtNCCjjLPFMU93vO6d9z3UsuvNWvUbSbDcjqH8N4Yyp1LI7RjGoBd7WYZyDdUhZD93EXi1B5TXqq17ZbXxBy7HgtM8i9zbHbcyzerO9HCodqLsHwd29lE</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>MASAKI, Takayuki</creator><creator>CHIBA, Seiichi</creator><creator>YASUDA, Tohru</creator><creator>TSUBONE, Tetsuo</creator><creator>KAKUMA, Tetsuya</creator><creator>SHIMOMURA, Iichiro</creator><creator>FUNAHASHI, Tohru</creator><creator>MATSUZAWA, Yuji</creator><creator>YOSHIMATSU, Hironobu</creator><general>American Diabetes Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow (Ay/a) Obese Mice</title><author>MASAKI, Takayuki ; CHIBA, Seiichi ; YASUDA, Tohru ; TSUBONE, Tetsuo ; KAKUMA, Tetsuya ; SHIMOMURA, Iichiro ; FUNAHASHI, Tohru ; MATSUZAWA, Yuji ; YOSHIMATSU, Hironobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c585t-a45840ae8ebfd38c631bd5bc93abaca4772cd0704991204be1275cdcb778fc723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adiponectin</topic><topic>Adipose tissue</topic><topic>Adipose Tissue, Brown - drug effects</topic><topic>Adipose tissues</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - drug effects</topic><topic>Body Temperature - drug effects</topic><topic>Body Weight - drug effects</topic><topic>Carrier Proteins - genetics</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Eating - drug effects</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Gene Expression - drug effects</topic><topic>Hypothalamus - drug effects</topic><topic>Hypothalamus - metabolism</topic><topic>Ingestion</topic><topic>Injections, Intraperitoneal</topic><topic>Injections, Intraventricular</topic><topic>Insulin - blood</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Ion Channels</topic><topic>Liver - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Transport Proteins</topic><topic>Metabolic diseases</topic><topic>Mice</topic><topic>Mice, Obese</topic><topic>Mitochondrial Proteins</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - physiology</topic><topic>Obesity</topic><topic>Obesity - drug therapy</topic><topic>Obesity - physiopathology</topic><topic>Physiological aspects</topic><topic>Proteins - genetics</topic><topic>Proteins - pharmacology</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>Triglycerides - blood</topic><topic>Uncoupling Protein 1</topic><topic>Uncoupling Protein 2</topic><topic>Up-Regulation - drug effects</topic><topic>Viscera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MASAKI, Takayuki</creatorcontrib><creatorcontrib>CHIBA, Seiichi</creatorcontrib><creatorcontrib>YASUDA, Tohru</creatorcontrib><creatorcontrib>TSUBONE, Tetsuo</creatorcontrib><creatorcontrib>KAKUMA, Tetsuya</creatorcontrib><creatorcontrib>SHIMOMURA, Iichiro</creatorcontrib><creatorcontrib>FUNAHASHI, Tohru</creatorcontrib><creatorcontrib>MATSUZAWA, Yuji</creatorcontrib><creatorcontrib>YOSHIMATSU, Hironobu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Diabetes (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MASAKI, Takayuki</au><au>CHIBA, Seiichi</au><au>YASUDA, Tohru</au><au>TSUBONE, Tetsuo</au><au>KAKUMA, Tetsuya</au><au>SHIMOMURA, Iichiro</au><au>FUNAHASHI, Tohru</au><au>MATSUZAWA, Yuji</au><au>YOSHIMATSU, Hironobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow (Ay/a) Obese Mice</atitle><jtitle>Diabetes (New York, N.Y.)</jtitle><addtitle>Diabetes</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>52</volume><issue>9</issue><spage>2266</spage><epage>2273</epage><pages>2266-2273</pages><issn>0012-1797</issn><eissn>1939-327X</eissn><coden>DIAEAZ</coden><abstract>Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling
Protein in Agouti Yellow ( A y /a ) Obese Mice
Takayuki Masaki 1 ,
Seiichi Chiba 1 ,
Tohru Yasuda 1 ,
Tetsuo Tsubone 1 ,
Tetsuya Kakuma 1 ,
Iichiro Shimomura 2 ,
Tohru Funahashi 3 ,
Yuji Matsuzawa 3 and
Hironobu Yoshimatsu 1
1 Department of Internal Medicine, School of Medicine, Oita Medical University, Oita, Japan
2 Department of Medicine and Pathophysiology, Graduate School of Medicine, Osaka University, Osaka, Japan
3 Department of Internal Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan
Address correspondence and reprint requests to Dr. Takayuki Masaki, Department of Internal Medicine, School of Medicine, Oita
Medical University, Hasama, Oita, 879-5593, Japan. E-mail: masaki{at}oita-med.ac.jp
Abstract
To examine the peripheral and central roles of adiponectin in energy intake and expenditure, we investigated the effects of
adiponectin on food intake, adiposity, sympathetic nerve activity (SNA), and mRNA expressions of uncoupling protein (UCP)
in the brown adipose tissue (BAT), white adipose tissue (WAT) and skeletal muscle in agouti yellow ( A y /a ) obese mice. Intraperitoneal administration of adiponectin (1.5 mg/kg for 7 days) attenuated body weight gain and reduced
visceral adiposity in A y /a obese mice compared with PBS-treated controls. In addition, adiponectin treatment increased the expression of UCP1 mRNA in
BAT, UCP2 mRNA in WAT, and UCP3 mRNA in skeletal muscle compared with PBS-treated A y /a controls. Acute peripheral administration of adiponectin (1.5 mg/kg, one injection) also increased SNA in the BAT accompanied
by an increase in rectal temperature. Finally, these above responses as well as expression of c-Fos–like immunohistochemistry
in the hypothalamus were not induced by central application of adiponectin (0–15 μg/kg). Taken together, adiponectin effectively
regulated visceral adiposity, SNA, and UCP mRNA expression peripherally, suggesting that this substance can be used as a therapeutic
tool, administered peripherally, in the treatment of visceral obesity and related metabolic disorders.
ARC, arcuate nucleus
BAT, brown adipose tissue
ICV, intracerebroventricular
PVN, paraventricular nucleus
SNA, sympathetic nerve activity
UCP, uncoupling protein
VMH, ventromedial hypothalamus
WAT, white adipose tissue
Footnotes
Accepted June 18, 2003.
Received March 18, 2003.
DIABETES</abstract><cop>Alexandria, VA</cop><pub>American Diabetes Association</pub><pmid>12941765</pmid><doi>10.2337/diabetes.52.9.2266</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1797 |
| ispartof | Diabetes (New York, N.Y.), 2003-09, Vol.52 (9), p.2266-2273 |
| issn | 0012-1797 1939-327X |
| language | eng |
| recordid | cdi_gale_incontextcollege_GICCO_A107754838 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adiponectin Adipose tissue Adipose Tissue, Brown - drug effects Adipose tissues Animals Biological and medical sciences Blood Glucose - drug effects Body Temperature - drug effects Body Weight - drug effects Carrier Proteins - genetics Diabetes Diabetes mellitus Diabetes. Impaired glucose tolerance Eating - drug effects Endocrine pancreas. Apud cells (diseases) Endocrinopathies Gene Expression - drug effects Hypothalamus - drug effects Hypothalamus - metabolism Ingestion Injections, Intraperitoneal Injections, Intraventricular Insulin - blood Intercellular Signaling Peptides and Proteins Ion Channels Liver - drug effects Male Medical sciences Membrane Proteins - genetics Membrane Transport Proteins Metabolic diseases Mice Mice, Obese Mitochondrial Proteins Muscle, Skeletal - drug effects Muscle, Skeletal - physiology Obesity Obesity - drug therapy Obesity - physiopathology Physiological aspects Proteins - genetics Proteins - pharmacology Proto-Oncogene Proteins c-fos - metabolism RNA, Messenger - analysis Triglycerides - blood Uncoupling Protein 1 Uncoupling Protein 2 Up-Regulation - drug effects Viscera |
| title | Peripheral, But Not Central, Administration of Adiponectin Reduces Visceral Adiposity and Upregulates the Expression of Uncoupling Protein in Agouti Yellow (Ay/a) Obese Mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T12%3A04%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_highw&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Peripheral,%20But%20Not%20Central,%20Administration%20of%20Adiponectin%20Reduces%20Visceral%20Adiposity%20and%20Upregulates%20the%20Expression%20of%20Uncoupling%20Protein%20in%20Agouti%20Yellow%20(Ay/a)%20Obese%20Mice&rft.jtitle=Diabetes%20(New%20York,%20N.Y.)&rft.au=MASAKI,%20Takayuki&rft.date=2003-09-01&rft.volume=52&rft.issue=9&rft.spage=2266&rft.epage=2273&rft.pages=2266-2273&rft.issn=0012-1797&rft.eissn=1939-327X&rft.coden=DIAEAZ&rft_id=info:doi/10.2337/diabetes.52.9.2266&rft_dat=%3Cgale_highw%3EA107754838%3C/gale_highw%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=216482160&rft_id=info:pmid/12941765&rft_galeid=A107754838&rfr_iscdi=true |