Proteomic profile of
We investigated a proteome profile, protein-protein interaction and morphological changes of after different times of eupomatenoid-5 (EUP-5) induction to evaluate the cellular response to the drug-induced damages. The bacillus was induced to sub-minimal inhibitory concentration of EUP-5 at 12 h, 24...
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| Veröffentlicht in: | Future microbiology 2017-08, Vol.12 (10), p.867-879 |
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| container_title | Future microbiology |
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| creator | Ghiraldi-Lopes, Luciana D Campanerut-Sá, Paula AZ Meneguello, Jean E Seixas, Flávio AV Lopes-Ortiz, Mariana A Scodro, Regiane BL Pires, Claudia TA da Silva, Rosi Z Siqueira, Vera LD Nakamura, Celso V Cardoso, Rosilene F |
| description | We investigated a proteome profile, protein-protein interaction and morphological changes of
after different times of eupomatenoid-5 (EUP-5) induction to evaluate the cellular response to the drug-induced damages.
The bacillus was induced to sub-minimal inhibitory concentration of EUP-5 at 12 h, 24 h and 48 h. The proteins were separated by 2D gel electrophoresis, identified by LC/MS-MS. Scanning electron microscopy and Search Tool for the Retrieval of Interacting Genes/Proteins analyses were performed.
EUP-5 impacts mainly in
proteins of intermediary metabolism and interactome suggests a multisite disturbance that contributes to bacilli death. Scanning electron microscopy revealed the loss of bacillary form.
Some of the differentially expressed proteins have the potential to be drug targets such as citrate synthase (Rv0896), phosphoglycerate kinase (Rv1437), ketol-acid reductoisomerase (Rv3001c) and ATP synthase alpha chain (Rv1308). |
| doi_str_mv | 10.2217/fmb-2017-0023 |
| format | Article |
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after different times of eupomatenoid-5 (EUP-5) induction to evaluate the cellular response to the drug-induced damages.
The bacillus was induced to sub-minimal inhibitory concentration of EUP-5 at 12 h, 24 h and 48 h. The proteins were separated by 2D gel electrophoresis, identified by LC/MS-MS. Scanning electron microscopy and Search Tool for the Retrieval of Interacting Genes/Proteins analyses were performed.
EUP-5 impacts mainly in
proteins of intermediary metabolism and interactome suggests a multisite disturbance that contributes to bacilli death. Scanning electron microscopy revealed the loss of bacillary form.
Some of the differentially expressed proteins have the potential to be drug targets such as citrate synthase (Rv0896), phosphoglycerate kinase (Rv1437), ketol-acid reductoisomerase (Rv3001c) and ATP synthase alpha chain (Rv1308).</description><identifier>ISSN: 1746-0913</identifier><identifier>EISSN: 1746-0921</identifier><identifier>DOI: 10.2217/fmb-2017-0023</identifier><language>eng</language><publisher>Future Medicine Ltd</publisher><subject>2D gel electrophoresis ; drug targets ; eupomatenoid-5 ; LC/MS-MS ; protein changes ; proteome ; scanning electron microscopy ; STRING database ; tuberculosis</subject><ispartof>Future microbiology, 2017-08, Vol.12 (10), p.867-879</ispartof><rights>Future Medicine Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Ghiraldi-Lopes, Luciana D</creatorcontrib><creatorcontrib>Campanerut-Sá, Paula AZ</creatorcontrib><creatorcontrib>Meneguello, Jean E</creatorcontrib><creatorcontrib>Seixas, Flávio AV</creatorcontrib><creatorcontrib>Lopes-Ortiz, Mariana A</creatorcontrib><creatorcontrib>Scodro, Regiane BL</creatorcontrib><creatorcontrib>Pires, Claudia TA</creatorcontrib><creatorcontrib>da Silva, Rosi Z</creatorcontrib><creatorcontrib>Siqueira, Vera LD</creatorcontrib><creatorcontrib>Nakamura, Celso V</creatorcontrib><creatorcontrib>Cardoso, Rosilene F</creatorcontrib><title>Proteomic profile of</title><title>Future microbiology</title><description>We investigated a proteome profile, protein-protein interaction and morphological changes of
after different times of eupomatenoid-5 (EUP-5) induction to evaluate the cellular response to the drug-induced damages.
The bacillus was induced to sub-minimal inhibitory concentration of EUP-5 at 12 h, 24 h and 48 h. The proteins were separated by 2D gel electrophoresis, identified by LC/MS-MS. Scanning electron microscopy and Search Tool for the Retrieval of Interacting Genes/Proteins analyses were performed.
EUP-5 impacts mainly in
proteins of intermediary metabolism and interactome suggests a multisite disturbance that contributes to bacilli death. Scanning electron microscopy revealed the loss of bacillary form.
Some of the differentially expressed proteins have the potential to be drug targets such as citrate synthase (Rv0896), phosphoglycerate kinase (Rv1437), ketol-acid reductoisomerase (Rv3001c) and ATP synthase alpha chain (Rv1308).</description><subject>2D gel electrophoresis</subject><subject>drug targets</subject><subject>eupomatenoid-5</subject><subject>LC/MS-MS</subject><subject>protein changes</subject><subject>proteome</subject><subject>scanning electron microscopy</subject><subject>STRING database</subject><subject>tuberculosis</subject><issn>1746-0913</issn><issn>1746-0921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNpjYBA1NNAzMjI010_LTdI1MjA01zUwMDJmYuA0NDcx0zWwNDJkgbMNjTkYuIqLswwMTC0MLQ05GUQCivJLUvNzM5MVCory0zJzUhXy03gYWNMSc4pTeaE0N4Olm2uIs4duWmlJaVFqcXJmal5yajyEl5uakpmcmZcab2gQD3JGPNAZ8SBnxIOcYUyJXgBFtj5T</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Ghiraldi-Lopes, Luciana D</creator><creator>Campanerut-Sá, Paula AZ</creator><creator>Meneguello, Jean E</creator><creator>Seixas, Flávio AV</creator><creator>Lopes-Ortiz, Mariana A</creator><creator>Scodro, Regiane BL</creator><creator>Pires, Claudia TA</creator><creator>da Silva, Rosi Z</creator><creator>Siqueira, Vera LD</creator><creator>Nakamura, Celso V</creator><creator>Cardoso, Rosilene F</creator><general>Future Medicine Ltd</general><scope/></search><sort><creationdate>20170801</creationdate><title>Proteomic profile of</title><author>Ghiraldi-Lopes, Luciana D ; Campanerut-Sá, Paula AZ ; Meneguello, Jean E ; Seixas, Flávio AV ; Lopes-Ortiz, Mariana A ; Scodro, Regiane BL ; Pires, Claudia TA ; da Silva, Rosi Z ; Siqueira, Vera LD ; Nakamura, Celso V ; Cardoso, Rosilene F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-futurescience_futuremedicine_10_2217_fmb_2017_00233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>2D gel electrophoresis</topic><topic>drug targets</topic><topic>eupomatenoid-5</topic><topic>LC/MS-MS</topic><topic>protein changes</topic><topic>proteome</topic><topic>scanning electron microscopy</topic><topic>STRING database</topic><topic>tuberculosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghiraldi-Lopes, Luciana D</creatorcontrib><creatorcontrib>Campanerut-Sá, Paula AZ</creatorcontrib><creatorcontrib>Meneguello, Jean E</creatorcontrib><creatorcontrib>Seixas, Flávio AV</creatorcontrib><creatorcontrib>Lopes-Ortiz, Mariana A</creatorcontrib><creatorcontrib>Scodro, Regiane BL</creatorcontrib><creatorcontrib>Pires, Claudia TA</creatorcontrib><creatorcontrib>da Silva, Rosi Z</creatorcontrib><creatorcontrib>Siqueira, Vera LD</creatorcontrib><creatorcontrib>Nakamura, Celso V</creatorcontrib><creatorcontrib>Cardoso, Rosilene F</creatorcontrib><jtitle>Future microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghiraldi-Lopes, Luciana D</au><au>Campanerut-Sá, Paula AZ</au><au>Meneguello, Jean E</au><au>Seixas, Flávio AV</au><au>Lopes-Ortiz, Mariana A</au><au>Scodro, Regiane BL</au><au>Pires, Claudia TA</au><au>da Silva, Rosi Z</au><au>Siqueira, Vera LD</au><au>Nakamura, Celso V</au><au>Cardoso, Rosilene F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic profile of</atitle><jtitle>Future microbiology</jtitle><date>2017-08-01</date><risdate>2017</risdate><volume>12</volume><issue>10</issue><spage>867</spage><epage>879</epage><pages>867-879</pages><issn>1746-0913</issn><eissn>1746-0921</eissn><abstract>We investigated a proteome profile, protein-protein interaction and morphological changes of
after different times of eupomatenoid-5 (EUP-5) induction to evaluate the cellular response to the drug-induced damages.
The bacillus was induced to sub-minimal inhibitory concentration of EUP-5 at 12 h, 24 h and 48 h. The proteins were separated by 2D gel electrophoresis, identified by LC/MS-MS. Scanning electron microscopy and Search Tool for the Retrieval of Interacting Genes/Proteins analyses were performed.
EUP-5 impacts mainly in
proteins of intermediary metabolism and interactome suggests a multisite disturbance that contributes to bacilli death. Scanning electron microscopy revealed the loss of bacillary form.
Some of the differentially expressed proteins have the potential to be drug targets such as citrate synthase (Rv0896), phosphoglycerate kinase (Rv1437), ketol-acid reductoisomerase (Rv3001c) and ATP synthase alpha chain (Rv1308).</abstract><pub>Future Medicine Ltd</pub><doi>10.2217/fmb-2017-0023</doi></addata></record> |
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| ispartof | Future microbiology, 2017-08, Vol.12 (10), p.867-879 |
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| language | eng |
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| source | PubMed Central |
| subjects | 2D gel electrophoresis drug targets eupomatenoid-5 LC/MS-MS protein changes proteome scanning electron microscopy STRING database tuberculosis |
| title | Proteomic profile of |
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