A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses
Intertypic recombinant fowl adenoviruses (FAVs) were generated to determine regions of the viral genome involved in virulence. Recombinants were produced with two serotype 8 FAVs, mildly virulent CFA 3 and hypervirulent CFA 40. Restriction endonuclease fragments from the genomes of the two FAVs were...
Gespeichert in:
| Veröffentlicht in: | Journal of Virology 1996-08, Vol.70 (8), p.5115-5122 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5122 |
|---|---|
| container_issue | 8 |
| container_start_page | 5115 |
| container_title | Journal of Virology |
| container_volume | 70 |
| creator | Pallister, J. (The Upjohn Company, Kalamazoo, MI.) Wright, P.J Sheppard, M |
| description | Intertypic recombinant fowl adenoviruses (FAVs) were generated to determine regions of the viral genome involved in virulence. Recombinants were produced with two serotype 8 FAVs, mildly virulent CFA 3 and hypervirulent CFA 40. Restriction endonuclease fragments from the genomes of the two FAVs were used to transfect primary chicken kidney cells. Virulence testing of these recombinants located the region responsible for differences in virulence to an 8.4-kb fragment of the genome located between kb 26.6 and 35.0. According to data available for a serotype 10 FAV that had been partially characterized in the laboratory, this segment of the genome contained three genes of known identity (100K, 33K, and pVIII) and a region between kb 31 and 35 with unknown coding potential (although this information subsequently became available for a serotype 1 FAV, CELO). Therefore, the region between kb 30.5 and 34.5 was sequenced. The results revealed that the unknown region encoded a fiber gene on the right strand and several small open reading frames of unknown identity on the left strand. Further recombinant viruses containing defined exchanges within the 4-kb fragment were constructed, and virulence testing of these viruses indicated that the fiber was responsible for differences in virulence for CFA 40 and CFA 3 |
| doi_str_mv | 10.1128/jvi.70.8.5115-5122.1996 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_fao_a</sourceid><recordid>TN_cdi_fao_agris_US9626312</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15590461</sourcerecordid><originalsourceid>FETCH-LOGICAL-c553t-65122bc3fe6c59c4d6a25634d7ca062624341b3615d9ea7bb66cd3c431d809f23</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS1EVZbCF0BCuBduCR7_S3LgUFXQIlXqoVTiZjnOJOsqGy92diu-PQ67quipJ2v8fm80M4-QT8BKAF5_edj7smJlXSoAVSjgvISm0a_IClhTF0qBfE1WjHFeKFH_ekPepvTAGEip5Sk5rSstGTQr0l3Q5KdhRDrghBQnF7pc03mNtPctRuoTjZi2YUq-zVgfIt3b6O3s8xf1E937uBuzEZfiny88jtR2OIVFSpjekZPejgnfH98zcv_928_L6-Lm9urH5cVN4ZQSc6GXNVonetRONU522nKlhewqZ5nmmkshoRUaVNegrdpWa9cJJwV0NWt6Ls7I10Pf7a7dYOdwmqMdzTb6jY1_TLDePFcmvzZD2BtomNQ6-z8f_TH83mGazcYnh-NoJwy7ZKqaVwIqeBEEpZaOC1gdQBdDShH7p2GAmSVIk4M0FTO1WYI0ywXMEmR2fvx_lyffMbmsnx_0tR_Wjz6isWnzvFtmPhyY3gZjh-iTub9r8iUFcPEX8pCv8Q</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15590461</pqid></control><display><type>article</type><title>A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Pallister, J. (The Upjohn Company, Kalamazoo, MI.) ; Wright, P.J ; Sheppard, M</creator><creatorcontrib>Pallister, J. (The Upjohn Company, Kalamazoo, MI.) ; Wright, P.J ; Sheppard, M</creatorcontrib><description>Intertypic recombinant fowl adenoviruses (FAVs) were generated to determine regions of the viral genome involved in virulence. Recombinants were produced with two serotype 8 FAVs, mildly virulent CFA 3 and hypervirulent CFA 40. Restriction endonuclease fragments from the genomes of the two FAVs were used to transfect primary chicken kidney cells. Virulence testing of these recombinants located the region responsible for differences in virulence to an 8.4-kb fragment of the genome located between kb 26.6 and 35.0. According to data available for a serotype 10 FAV that had been partially characterized in the laboratory, this segment of the genome contained three genes of known identity (100K, 33K, and pVIII) and a region between kb 31 and 35 with unknown coding potential (although this information subsequently became available for a serotype 1 FAV, CELO). Therefore, the region between kb 30.5 and 34.5 was sequenced. The results revealed that the unknown region encoded a fiber gene on the right strand and several small open reading frames of unknown identity on the left strand. Further recombinant viruses containing defined exchanges within the 4-kb fragment were constructed, and virulence testing of these viruses indicated that the fiber was responsible for differences in virulence for CFA 40 and CFA 3</description><identifier>ISSN: 0022-538X</identifier><identifier>EISSN: 1098-5514</identifier><identifier>DOI: 10.1128/jvi.70.8.5115-5122.1996</identifier><identifier>PMID: 8764019</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Adenoviridae - genetics ; Adenoviridae - pathogenicity ; Animals ; AVIADENOVIRUS ; Birds ; Capsid - genetics ; Capsid Proteins ; Chromosome Mapping ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; DNA, Recombinant ; EXPERIMENTACION IN VIVO ; EXPERIMENTATION IN VIVO ; GENE ; GENES ; GENETICA ; GENETIQUE ; Genome, Viral ; INFECCION EXPERIMENTAL ; INFECTION EXPERIMENTALE ; Molecular Sequence Data ; PATOGENICIDAD ; POLLITO ; POUSSIN ; POUVOIR PATHOGENE ; PROTEINAS ; PROTEINE ; RECOMBINACION ; RECOMBINAISON ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; Virulence - genetics ; VIRUS DE LOS ANIMALES ; VIRUS DES ANIMAUX</subject><ispartof>Journal of Virology, 1996-08, Vol.70 (8), p.5115-5122</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-65122bc3fe6c59c4d6a25634d7ca062624341b3615d9ea7bb66cd3c431d809f23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC190466/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC190466/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8764019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pallister, J. (The Upjohn Company, Kalamazoo, MI.)</creatorcontrib><creatorcontrib>Wright, P.J</creatorcontrib><creatorcontrib>Sheppard, M</creatorcontrib><title>A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses</title><title>Journal of Virology</title><addtitle>J Virol</addtitle><description>Intertypic recombinant fowl adenoviruses (FAVs) were generated to determine regions of the viral genome involved in virulence. Recombinants were produced with two serotype 8 FAVs, mildly virulent CFA 3 and hypervirulent CFA 40. Restriction endonuclease fragments from the genomes of the two FAVs were used to transfect primary chicken kidney cells. Virulence testing of these recombinants located the region responsible for differences in virulence to an 8.4-kb fragment of the genome located between kb 26.6 and 35.0. According to data available for a serotype 10 FAV that had been partially characterized in the laboratory, this segment of the genome contained three genes of known identity (100K, 33K, and pVIII) and a region between kb 31 and 35 with unknown coding potential (although this information subsequently became available for a serotype 1 FAV, CELO). Therefore, the region between kb 30.5 and 34.5 was sequenced. The results revealed that the unknown region encoded a fiber gene on the right strand and several small open reading frames of unknown identity on the left strand. Further recombinant viruses containing defined exchanges within the 4-kb fragment were constructed, and virulence testing of these viruses indicated that the fiber was responsible for differences in virulence for CFA 40 and CFA 3</description><subject>Adenoviridae - genetics</subject><subject>Adenoviridae - pathogenicity</subject><subject>Animals</subject><subject>AVIADENOVIRUS</subject><subject>Birds</subject><subject>Capsid - genetics</subject><subject>Capsid Proteins</subject><subject>Chromosome Mapping</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>DNA, Recombinant</subject><subject>EXPERIMENTACION IN VIVO</subject><subject>EXPERIMENTATION IN VIVO</subject><subject>GENE</subject><subject>GENES</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Genome, Viral</subject><subject>INFECCION EXPERIMENTAL</subject><subject>INFECTION EXPERIMENTALE</subject><subject>Molecular Sequence Data</subject><subject>PATOGENICIDAD</subject><subject>POLLITO</subject><subject>POUSSIN</subject><subject>POUVOIR PATHOGENE</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>Virulence - genetics</subject><subject>VIRUS DE LOS ANIMALES</subject><subject>VIRUS DES ANIMAUX</subject><issn>0022-538X</issn><issn>1098-5514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1EVZbCF0BCuBduCR7_S3LgUFXQIlXqoVTiZjnOJOsqGy92diu-PQ67quipJ2v8fm80M4-QT8BKAF5_edj7smJlXSoAVSjgvISm0a_IClhTF0qBfE1WjHFeKFH_ekPepvTAGEip5Sk5rSstGTQr0l3Q5KdhRDrghBQnF7pc03mNtPctRuoTjZi2YUq-zVgfIt3b6O3s8xf1E937uBuzEZfiny88jtR2OIVFSpjekZPejgnfH98zcv_928_L6-Lm9urH5cVN4ZQSc6GXNVonetRONU522nKlhewqZ5nmmkshoRUaVNegrdpWa9cJJwV0NWt6Ls7I10Pf7a7dYOdwmqMdzTb6jY1_TLDePFcmvzZD2BtomNQ6-z8f_TH83mGazcYnh-NoJwy7ZKqaVwIqeBEEpZaOC1gdQBdDShH7p2GAmSVIk4M0FTO1WYI0ywXMEmR2fvx_lyffMbmsnx_0tR_Wjz6isWnzvFtmPhyY3gZjh-iTub9r8iUFcPEX8pCv8Q</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>Pallister, J. (The Upjohn Company, Kalamazoo, MI.)</creator><creator>Wright, P.J</creator><creator>Sheppard, M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960801</creationdate><title>A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses</title><author>Pallister, J. (The Upjohn Company, Kalamazoo, MI.) ; Wright, P.J ; Sheppard, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c553t-65122bc3fe6c59c4d6a25634d7ca062624341b3615d9ea7bb66cd3c431d809f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adenoviridae - genetics</topic><topic>Adenoviridae - pathogenicity</topic><topic>Animals</topic><topic>AVIADENOVIRUS</topic><topic>Birds</topic><topic>Capsid - genetics</topic><topic>Capsid Proteins</topic><topic>Chromosome Mapping</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>DNA, Recombinant</topic><topic>EXPERIMENTACION IN VIVO</topic><topic>EXPERIMENTATION IN VIVO</topic><topic>GENE</topic><topic>GENES</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Genome, Viral</topic><topic>INFECCION EXPERIMENTAL</topic><topic>INFECTION EXPERIMENTALE</topic><topic>Molecular Sequence Data</topic><topic>PATOGENICIDAD</topic><topic>POLLITO</topic><topic>POUSSIN</topic><topic>POUVOIR PATHOGENE</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>Virulence - genetics</topic><topic>VIRUS DE LOS ANIMALES</topic><topic>VIRUS DES ANIMAUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pallister, J. (The Upjohn Company, Kalamazoo, MI.)</creatorcontrib><creatorcontrib>Wright, P.J</creatorcontrib><creatorcontrib>Sheppard, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pallister, J. (The Upjohn Company, Kalamazoo, MI.)</au><au>Wright, P.J</au><au>Sheppard, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>70</volume><issue>8</issue><spage>5115</spage><epage>5122</epage><pages>5115-5122</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>Intertypic recombinant fowl adenoviruses (FAVs) were generated to determine regions of the viral genome involved in virulence. Recombinants were produced with two serotype 8 FAVs, mildly virulent CFA 3 and hypervirulent CFA 40. Restriction endonuclease fragments from the genomes of the two FAVs were used to transfect primary chicken kidney cells. Virulence testing of these recombinants located the region responsible for differences in virulence to an 8.4-kb fragment of the genome located between kb 26.6 and 35.0. According to data available for a serotype 10 FAV that had been partially characterized in the laboratory, this segment of the genome contained three genes of known identity (100K, 33K, and pVIII) and a region between kb 31 and 35 with unknown coding potential (although this information subsequently became available for a serotype 1 FAV, CELO). Therefore, the region between kb 30.5 and 34.5 was sequenced. The results revealed that the unknown region encoded a fiber gene on the right strand and several small open reading frames of unknown identity on the left strand. Further recombinant viruses containing defined exchanges within the 4-kb fragment were constructed, and virulence testing of these viruses indicated that the fiber was responsible for differences in virulence for CFA 40 and CFA 3</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>8764019</pmid><doi>10.1128/jvi.70.8.5115-5122.1996</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-538X |
| ispartof | Journal of Virology, 1996-08, Vol.70 (8), p.5115-5122 |
| issn | 0022-538X 1098-5514 |
| language | eng |
| recordid | cdi_fao_agris_US9626312 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adenoviridae - genetics Adenoviridae - pathogenicity Animals AVIADENOVIRUS Birds Capsid - genetics Capsid Proteins Chromosome Mapping COMPOSICION QUIMICA COMPOSITION CHIMIQUE DNA, Recombinant EXPERIMENTACION IN VIVO EXPERIMENTATION IN VIVO GENE GENES GENETICA GENETIQUE Genome, Viral INFECCION EXPERIMENTAL INFECTION EXPERIMENTALE Molecular Sequence Data PATOGENICIDAD POLLITO POUSSIN POUVOIR PATHOGENE PROTEINAS PROTEINE RECOMBINACION RECOMBINAISON SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS Virulence - genetics VIRUS DE LOS ANIMALES VIRUS DES ANIMAUX |
| title | A single gene encoding the fiber is responsible for variations in virulence in the fowl adenoviruses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T08%3A23%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_fao_a&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20single%20gene%20encoding%20the%20fiber%20is%20responsible%20for%20variations%20in%20virulence%20in%20the%20fowl%20adenoviruses&rft.jtitle=Journal%20of%20Virology&rft.au=Pallister,%20J.%20(The%20Upjohn%20Company,%20Kalamazoo,%20MI.)&rft.date=1996-08-01&rft.volume=70&rft.issue=8&rft.spage=5115&rft.epage=5122&rft.pages=5115-5122&rft.issn=0022-538X&rft.eissn=1098-5514&rft_id=info:doi/10.1128/jvi.70.8.5115-5122.1996&rft_dat=%3Cproquest_fao_a%3E15590461%3C/proquest_fao_a%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15590461&rft_id=info:pmid/8764019&rfr_iscdi=true |