Transcriptional enhancer activity of hr5 requires dual-palindrome half sites that mediate binding of a dimeric form of the baculovirus transregulator IE1

The hr5 enhancer element stimulates early viral transcription and may function as an origin of DNA replication for Autographa californica nuclear polyhedrosis virus (AcMNPV). The smallest functional unit of hr5 is a 28-bp repeat consisting of an imperfect palindrome (28-mer). To identify essential s...

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Veröffentlicht in:	Journal of Virology 1995-09, Vol.69 (9), p.5368-5375
Hauptverfasser:	Rodems, S.M. (University of California, San Diego, La Jolla, CA.), Friesen, P.D
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Animals AUTOGRAPHA CALIFORNICA baculovirus Base Sequence Binding Sites Binding, Competitive Cell Line Cell Nucleus - metabolism DNA Primers DNA Probes DNA, Viral - metabolism DNA-Binding Proteins Enhancer Elements, Genetic EXPRESION GENICA EXPRESSION DES GENES GENE Gene Expression Regulation, Viral GENES GENETICA GENETIQUE Immediate-Early Proteins - chemistry Immediate-Early Proteins - metabolism Molecular Sequence Data MUTACION MUTACION INDUCIDA MUTATION MUTATION PROVOQUEE nuclear polyhedrosis virus Nucleopolyhedrovirus - genetics Nucleopolyhedrovirus - metabolism Plasmids Polymerase Chain Reaction Promoter Regions, Genetic PROTEINAS AGLUTINANTES PROTEINE DE LIAISON SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Spodoptera Trans-Activators - chemistry Trans-Activators - metabolism Transcriptional Activation VIRUS POLIEDROSIS NUCLEAR VIRUS POLYEDROSE NUCLEAIRE
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