Overexpression and characterization of a gene for a Ca2+-ATPase of the endoplasmic reticulum in Trypanosoma brucei

Procyclic forms of Trypanosoma brucei were stably transformed with an expression vector containing a gene for a P-type ATPase (tba1) cloned from T. brucei genomic DNA. Transformation with this gene resulted specifically in a 4-5-fold increase in the cellular Ca2+-ATPase activity. Subcellular fractio...

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Veröffentlicht in:The Journal of biological chemistry 1994-10, Vol.269 (42)
Hauptverfasser: Nolan, D.P. (University of Brussels, Rhode St. Genese, Belgium.), Revelard, P, Pays, E
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Sprache:eng
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