Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm
Lysine is the most limiting essential amino acid in cereals, and for many years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains. The opaque2 mutation in maize doubles the lysine content in the endosperm, but the mechanism by which this o...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1995-09, Vol.92 (19), p.8640-8644 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Habben, J.E. (University of Arizona, Tucson, AZ.) Moro, G.L Hunter, B.G Hamaker, B.R Larkins, B.A |
| description | Lysine is the most limiting essential amino acid in cereals, and for many years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains. The opaque2 mutation in maize doubles the lysine content in the endosperm, but the mechanism by which this occurs is unknown. We show that elongation factor 1 alpha (EF-1 alpha) is overexpressed in opaque2 endosperm compared with its normal counterpart and that there is a highly significant correlation between EF-1 alpha concentration and the total lysine content of the endosperm. This relationship is also true for two other cereals, sorghum and barley. It appears that genetic selection for genotypes with a high concentration of EF-1 alpha can significantly improve the nutritional quality of maize and other cereals |
| doi_str_mv | 10.1073/pnas.92.19.8640 |
| format | Article |
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(University of Arizona, Tucson, AZ.) ; Moro, G.L ; Hunter, B.G ; Hamaker, B.R ; Larkins, B.A</creator><creatorcontrib>Habben, J.E. (University of Arizona, Tucson, AZ.) ; Moro, G.L ; Hunter, B.G ; Hamaker, B.R ; Larkins, B.A</creatorcontrib><description>Lysine is the most limiting essential amino acid in cereals, and for many years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains. The opaque2 mutation in maize doubles the lysine content in the endosperm, but the mechanism by which this occurs is unknown. We show that elongation factor 1 alpha (EF-1 alpha) is overexpressed in opaque2 endosperm compared with its normal counterpart and that there is a highly significant correlation between EF-1 alpha concentration and the total lysine content of the endosperm. This relationship is also true for two other cereals, sorghum and barley. It appears that genetic selection for genotypes with a high concentration of EF-1 alpha can significantly improve the nutritional quality of maize and other cereals</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.92.19.8640</identifier><identifier>PMID: 7567989</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino acids ; Antibodies ; Antiserum ; Barley ; CALIDAD PROTEICA ; CEBADA ; Cereal grains ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; Corn ; Electrophoresis, Gel, Two-Dimensional ; Endosperm ; ENDOSPERMA ; ENDOSPERME ; Enzyme-Linked Immunosorbent Assay ; Genotype ; Genotypes ; Hordeum - chemistry ; HORDEUM VULGARE ; Immunoblotting ; LISINA ; LYSINE ; Lysine - analysis ; Mutation ; Nutritive Value ; ORGE ; Peptide Elongation Factor 1 ; Peptide elongation factors ; Peptide Elongation Factors - analysis ; Peptide Elongation Factors - genetics ; Plant Proteins - analysis ; Plant Proteins - genetics ; Poaceae - chemistry ; PROTEINAS ; PROTEINAS AGLUTINANTES ; PROTEINE ; PROTEINE DE LIAISON ; QUALITE PROTEIQUE ; Recombinant Fusion Proteins ; RIBOSOMAS ; RIBOSOME ; Seeds - chemistry ; Seeds - genetics ; SORGHUM ; SORGHUM BICOLOR ; Species Specificity ; ZEA MAYS ; Zea mays - chemistry ; Zea mays - genetics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1995-09, Vol.92 (19), p.8640-8644</ispartof><rights>Copyright 1995 The National Academy of Sciences of the United States of America</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-b07aa7af5e2f1ccad6d26eb5b55abc9db99852d78622fcbd04062554e8b0ab363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/92/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2368318$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2368318$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7567989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Habben, J.E. (University of Arizona, Tucson, AZ.)</creatorcontrib><creatorcontrib>Moro, G.L</creatorcontrib><creatorcontrib>Hunter, B.G</creatorcontrib><creatorcontrib>Hamaker, B.R</creatorcontrib><creatorcontrib>Larkins, B.A</creatorcontrib><title>Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Lysine is the most limiting essential amino acid in cereals, and for many years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains. The opaque2 mutation in maize doubles the lysine content in the endosperm, but the mechanism by which this occurs is unknown. We show that elongation factor 1 alpha (EF-1 alpha) is overexpressed in opaque2 endosperm compared with its normal counterpart and that there is a highly significant correlation between EF-1 alpha concentration and the total lysine content of the endosperm. This relationship is also true for two other cereals, sorghum and barley. It appears that genetic selection for genotypes with a high concentration of EF-1 alpha can significantly improve the nutritional quality of maize and other cereals</description><subject>Amino acids</subject><subject>Antibodies</subject><subject>Antiserum</subject><subject>Barley</subject><subject>CALIDAD PROTEICA</subject><subject>CEBADA</subject><subject>Cereal grains</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>Corn</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Endosperm</subject><subject>ENDOSPERMA</subject><subject>ENDOSPERME</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Hordeum - chemistry</subject><subject>HORDEUM VULGARE</subject><subject>Immunoblotting</subject><subject>LISINA</subject><subject>LYSINE</subject><subject>Lysine - analysis</subject><subject>Mutation</subject><subject>Nutritive Value</subject><subject>ORGE</subject><subject>Peptide Elongation Factor 1</subject><subject>Peptide elongation factors</subject><subject>Peptide Elongation Factors - analysis</subject><subject>Peptide Elongation Factors - genetics</subject><subject>Plant Proteins - analysis</subject><subject>Plant Proteins - genetics</subject><subject>Poaceae - chemistry</subject><subject>PROTEINAS</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINE</subject><subject>PROTEINE DE LIAISON</subject><subject>QUALITE PROTEIQUE</subject><subject>Recombinant Fusion Proteins</subject><subject>RIBOSOMAS</subject><subject>RIBOSOME</subject><subject>Seeds - chemistry</subject><subject>Seeds - genetics</subject><subject>SORGHUM</subject><subject>SORGHUM BICOLOR</subject><subject>Species Specificity</subject><subject>ZEA MAYS</subject><subject>Zea mays - chemistry</subject><subject>Zea mays - genetics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2L1DAYh4Mo67h6FkQlJz11NkmbL_Aiy_oBCx50zyFN0zZL2oxJZnX8603pOLgXPQXe5_m95OUHwHOMthjx-mI367SVZIvlVrAGPQAbjCSuWCPRQ7BBiPBKNKR5DJ6kdIsQklSgM3DGKeNSyA0YrnyYB51dmGGvTQ4RYqj9btTQhNnYOccVugRHN4z-UOYxWq-z7eAPl0eYRwv9IbnZLpFcIjD0cNLul4V27kLa2Tg9BY967ZN9dnzPwc2Hq2-Xn6rrLx8_X76_rgzFJFct4lpz3VNLemyM7lhHmG1pS6lujexaKQUlHReMkN60HWoQI5Q2VrRItzWrz8G7de9u3062Ww_wahfdpONBBe3UfTK7UQ3hTjUYEVLib47xGL7vbcpqcslY7_Vswz4pzilhTPxfxExwVBop4sUqmhhSirY__QUjtVSolgqVJApLtVRYEq_-PuHkHzsr_PWRL8E_9N6Ct_8UVL_3PtufuZgvV_M2leZPKqmZqLEo-MWKex2UHqJL6uarpAxTIurflA3E-w</recordid><startdate>19950912</startdate><enddate>19950912</enddate><creator>Habben, J.E. (University of Arizona, Tucson, AZ.)</creator><creator>Moro, G.L</creator><creator>Hunter, B.G</creator><creator>Hamaker, B.R</creator><creator>Larkins, B.A</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950912</creationdate><title>Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm</title><author>Habben, J.E. (University of Arizona, Tucson, AZ.) ; Moro, G.L ; Hunter, B.G ; Hamaker, B.R ; Larkins, B.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-b07aa7af5e2f1ccad6d26eb5b55abc9db99852d78622fcbd04062554e8b0ab363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino acids</topic><topic>Antibodies</topic><topic>Antiserum</topic><topic>Barley</topic><topic>CALIDAD PROTEICA</topic><topic>CEBADA</topic><topic>Cereal grains</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>Corn</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Endosperm</topic><topic>ENDOSPERMA</topic><topic>ENDOSPERME</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Hordeum - chemistry</topic><topic>HORDEUM VULGARE</topic><topic>Immunoblotting</topic><topic>LISINA</topic><topic>LYSINE</topic><topic>Lysine - analysis</topic><topic>Mutation</topic><topic>Nutritive Value</topic><topic>ORGE</topic><topic>Peptide Elongation Factor 1</topic><topic>Peptide elongation factors</topic><topic>Peptide Elongation Factors - analysis</topic><topic>Peptide Elongation Factors - genetics</topic><topic>Plant Proteins - analysis</topic><topic>Plant Proteins - genetics</topic><topic>Poaceae - chemistry</topic><topic>PROTEINAS</topic><topic>PROTEINAS AGLUTINANTES</topic><topic>PROTEINE</topic><topic>PROTEINE DE LIAISON</topic><topic>QUALITE PROTEIQUE</topic><topic>Recombinant Fusion Proteins</topic><topic>RIBOSOMAS</topic><topic>RIBOSOME</topic><topic>Seeds - chemistry</topic><topic>Seeds - genetics</topic><topic>SORGHUM</topic><topic>SORGHUM BICOLOR</topic><topic>Species Specificity</topic><topic>ZEA MAYS</topic><topic>Zea mays - chemistry</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Habben, J.E. (University of Arizona, Tucson, AZ.)</creatorcontrib><creatorcontrib>Moro, G.L</creatorcontrib><creatorcontrib>Hunter, B.G</creatorcontrib><creatorcontrib>Hamaker, B.R</creatorcontrib><creatorcontrib>Larkins, B.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Habben, J.E. (University of Arizona, Tucson, AZ.)</au><au>Moro, G.L</au><au>Hunter, B.G</au><au>Hamaker, B.R</au><au>Larkins, B.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1995-09-12</date><risdate>1995</risdate><volume>92</volume><issue>19</issue><spage>8640</spage><epage>8644</epage><pages>8640-8644</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Lysine is the most limiting essential amino acid in cereals, and for many years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains. The opaque2 mutation in maize doubles the lysine content in the endosperm, but the mechanism by which this occurs is unknown. We show that elongation factor 1 alpha (EF-1 alpha) is overexpressed in opaque2 endosperm compared with its normal counterpart and that there is a highly significant correlation between EF-1 alpha concentration and the total lysine content of the endosperm. This relationship is also true for two other cereals, sorghum and barley. It appears that genetic selection for genotypes with a high concentration of EF-1 alpha can significantly improve the nutritional quality of maize and other cereals</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>7567989</pmid><doi>10.1073/pnas.92.19.8640</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1995-09, Vol.92 (19), p.8640-8644 |
| issn | 0027-8424 1091-6490 |
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| recordid | cdi_fao_agris_US9561528 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino acids Antibodies Antiserum Barley CALIDAD PROTEICA CEBADA Cereal grains COMPOSICION QUIMICA COMPOSITION CHIMIQUE Corn Electrophoresis, Gel, Two-Dimensional Endosperm ENDOSPERMA ENDOSPERME Enzyme-Linked Immunosorbent Assay Genotype Genotypes Hordeum - chemistry HORDEUM VULGARE Immunoblotting LISINA LYSINE Lysine - analysis Mutation Nutritive Value ORGE Peptide Elongation Factor 1 Peptide elongation factors Peptide Elongation Factors - analysis Peptide Elongation Factors - genetics Plant Proteins - analysis Plant Proteins - genetics Poaceae - chemistry PROTEINAS PROTEINAS AGLUTINANTES PROTEINE PROTEINE DE LIAISON QUALITE PROTEIQUE Recombinant Fusion Proteins RIBOSOMAS RIBOSOME Seeds - chemistry Seeds - genetics SORGHUM SORGHUM BICOLOR Species Specificity ZEA MAYS Zea mays - chemistry Zea mays - genetics |
| title | Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm |
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