Identification and characterization of a novel Bradyrhizobium japonicum gene involved in host-specific nitrogen fixation
To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic development. This paper focuses on the little-understood genetics of host-specific nitrogen fixation. A...
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| Veröffentlicht in: | Journal of Bacteriology 1994-11, Vol.176 (21), p.6717-6729 |
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| creator | Chun, J.Y. (University of California, Berkeley, Berkeley, CA.) Sexton, G.L Roth, L.E Stacey, G |
| description | To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic development. This paper focuses on the little-understood genetics of host-specific nitrogen fixation. A deletion mutant of Bradyrhizobium japonicum, strain NAD163, was found to induce effective, nitrogen-fixing nodules on soybean and siratro plants but produced ineffective nodules on cowpea plants. Additional transposon and deletion mutants defined a small region that conferred this phenotype, and this region was sequenced to identify two putative open reading frames (ORFs). Data indicate that only one of these ORFs is detectable in bacteroids. This ORF was termed hsfA, with a predicted protein product of 11 kDa. The transcriptional start site of hsfA was determined and found to coincide with a predicted RpoN-dependent promoter. Microscopic studies of nodules induced by the wild type and hsfA mutants on cowpea and soybean plants indicate that the cowpea mutant nodules are slow to develop. The data indicate that hsfA appears to play a crucial role in bacteroid development on cowpea but does not appear to be essential for nitrogen fixation on the other hosts tested |
| doi_str_mv | 10.1128/JB.176.21.6717-6729.1994 |
| format | Article |
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(University of California, Berkeley, Berkeley, CA.) ; Sexton, G.L ; Roth, L.E ; Stacey, G</creator><creatorcontrib>Chun, J.Y. (University of California, Berkeley, Berkeley, CA.) ; Sexton, G.L ; Roth, L.E ; Stacey, G</creatorcontrib><description>To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic development. This paper focuses on the little-understood genetics of host-specific nitrogen fixation. A deletion mutant of Bradyrhizobium japonicum, strain NAD163, was found to induce effective, nitrogen-fixing nodules on soybean and siratro plants but produced ineffective nodules on cowpea plants. Additional transposon and deletion mutants defined a small region that conferred this phenotype, and this region was sequenced to identify two putative open reading frames (ORFs). Data indicate that only one of these ORFs is detectable in bacteroids. This ORF was termed hsfA, with a predicted protein product of 11 kDa. The transcriptional start site of hsfA was determined and found to coincide with a predicted RpoN-dependent promoter. Microscopic studies of nodules induced by the wild type and hsfA mutants on cowpea and soybean plants indicate that the cowpea mutant nodules are slow to develop. The data indicate that hsfA appears to play a crucial role in bacteroid development on cowpea but does not appear to be essential for nitrogen fixation on the other hosts tested</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/JB.176.21.6717-6729.1994</identifier><identifier>PMID: 7961425</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>ACETILENO ; ACETYLENE ; Amino Acid Sequence ; BACTERIA ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; BRADYRHIZOBIUM ; Bradyrhizobium japonicum ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fabaceae - microbiology ; Fabaceae - ultrastructure ; FIJACION DEL NITROGENO ; FIXATION DE L'AZOTE ; FLORA MICROBIANA ; FLORE MICROBIENNE ; GENE ; GENES ; Genes, Bacterial - genetics ; Genetics ; GLYCINE MAX ; Molecular Sequence Data ; Mutagenesis ; Mutation ; Nitrogen ; Nitrogen Fixation - genetics ; NODOSITE RACINAIRE ; NUDOSIDADES RADICULARES ; Phenotype ; Plant Roots - microbiology ; Plant Roots - ultrastructure ; Plant Tumors - microbiology ; Plants - microbiology ; Plants - ultrastructure ; Plants, Medicinal ; PROTEINAS ; PROTEINE ; REDUCCION ; REDUCTION ; Rhizobiaceae - genetics ; Rhizobiaceae - ultrastructure ; SECUENCIA NUCLEICA ; Sequence Analysis, DNA ; Sequence Deletion ; SEQUENCE NUCLEIQUE ; Species Specificity ; Symbiosis - genetics ; Transcription, Genetic ; VIGNA UNGUICULATA</subject><ispartof>Journal of Bacteriology, 1994-11, Vol.176 (21), p.6717-6729</ispartof><rights>Copyright American Society for Microbiology Nov 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-631da1fd32e3a29aab95bb1d1986df9713e3974733a465d5118f429fad3be6c43</citedby><cites>FETCH-LOGICAL-c518t-631da1fd32e3a29aab95bb1d1986df9713e3974733a465d5118f429fad3be6c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC197029/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC197029/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7961425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chun, J.Y. (University of California, Berkeley, Berkeley, CA.)</creatorcontrib><creatorcontrib>Sexton, G.L</creatorcontrib><creatorcontrib>Roth, L.E</creatorcontrib><creatorcontrib>Stacey, G</creatorcontrib><title>Identification and characterization of a novel Bradyrhizobium japonicum gene involved in host-specific nitrogen fixation</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic development. This paper focuses on the little-understood genetics of host-specific nitrogen fixation. A deletion mutant of Bradyrhizobium japonicum, strain NAD163, was found to induce effective, nitrogen-fixing nodules on soybean and siratro plants but produced ineffective nodules on cowpea plants. Additional transposon and deletion mutants defined a small region that conferred this phenotype, and this region was sequenced to identify two putative open reading frames (ORFs). Data indicate that only one of these ORFs is detectable in bacteroids. This ORF was termed hsfA, with a predicted protein product of 11 kDa. The transcriptional start site of hsfA was determined and found to coincide with a predicted RpoN-dependent promoter. Microscopic studies of nodules induced by the wild type and hsfA mutants on cowpea and soybean plants indicate that the cowpea mutant nodules are slow to develop. The data indicate that hsfA appears to play a crucial role in bacteroid development on cowpea but does not appear to be essential for nitrogen fixation on the other hosts tested</description><subject>ACETILENO</subject><subject>ACETYLENE</subject><subject>Amino Acid Sequence</subject><subject>BACTERIA</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>BRADYRHIZOBIUM</subject><subject>Bradyrhizobium japonicum</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fabaceae - microbiology</subject><subject>Fabaceae - ultrastructure</subject><subject>FIJACION DEL NITROGENO</subject><subject>FIXATION DE L'AZOTE</subject><subject>FLORA MICROBIANA</subject><subject>FLORE MICROBIENNE</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetics</subject><subject>GLYCINE MAX</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Mutation</subject><subject>Nitrogen</subject><subject>Nitrogen Fixation - genetics</subject><subject>NODOSITE RACINAIRE</subject><subject>NUDOSIDADES RADICULARES</subject><subject>Phenotype</subject><subject>Plant Roots - microbiology</subject><subject>Plant Roots - ultrastructure</subject><subject>Plant Tumors - microbiology</subject><subject>Plants - microbiology</subject><subject>Plants - ultrastructure</subject><subject>Plants, Medicinal</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>REDUCCION</subject><subject>REDUCTION</subject><subject>Rhizobiaceae - genetics</subject><subject>Rhizobiaceae - ultrastructure</subject><subject>SECUENCIA NUCLEICA</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Deletion</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Species Specificity</subject><subject>Symbiosis - genetics</subject><subject>Transcription, Genetic</subject><subject>VIGNA UNGUICULATA</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU9v1DAQxSMEKkvhCyAhWRy4JXjsJI4PHGgFtKgSB-jZmsTOxqusvdjJ9s-nx-muCvTkkf17b2b8sowALQBY8_H7WQGiLhgUtQCR14LJAqQsn2UroLLJq4rT59mKUga5BMlfZq9i3FAKZVmxk-xEyBpKVq2y20tt3GR72-FkvSPoNOkGDNhNJtj7w6XvCRLn92YkZwH1XRjsvW_tvCUb3Hlnu1StjTPEur0f90anggw-TnncmW4xJ85OwSeG9Pb2wfR19qLHMZo3x_M0u_765df5RX7149vl-eervKugmfKag0boNWeGI5OIrazaFjTIpta9FMANl6IUnGNZV7oCaPqSyR41b03dlfw0-3Tw3c3t1ugubRtwVLtgtxjulEer_n9xdlBrv1cgBWUy6T8c9cH_nk2c1NbGzowjOuPnqKCuhagakcD3T8CNn4NLuynGBE2_TWmCmgPUBR9jMP3jIEDVkqzatColqxioJVm1JKuWZJP03b-LPAqPUf7tP9j1cGODURi3T-wS9PYA9egVroON6vqnrHgakPI_6Wy2mQ</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Chun, J.Y. (University of California, Berkeley, Berkeley, CA.)</creator><creator>Sexton, G.L</creator><creator>Roth, L.E</creator><creator>Stacey, G</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>5PM</scope></search><sort><creationdate>19941101</creationdate><title>Identification and characterization of a novel Bradyrhizobium japonicum gene involved in host-specific nitrogen fixation</title><author>Chun, J.Y. (University of California, Berkeley, Berkeley, CA.) ; Sexton, G.L ; Roth, L.E ; Stacey, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-631da1fd32e3a29aab95bb1d1986df9713e3974733a465d5118f429fad3be6c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ACETILENO</topic><topic>ACETYLENE</topic><topic>Amino Acid Sequence</topic><topic>BACTERIA</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>BRADYRHIZOBIUM</topic><topic>Bradyrhizobium japonicum</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fabaceae - microbiology</topic><topic>Fabaceae - ultrastructure</topic><topic>FIJACION DEL NITROGENO</topic><topic>FIXATION DE L'AZOTE</topic><topic>FLORA MICROBIANA</topic><topic>FLORE MICROBIENNE</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetics</topic><topic>GLYCINE MAX</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Mutation</topic><topic>Nitrogen</topic><topic>Nitrogen Fixation - genetics</topic><topic>NODOSITE RACINAIRE</topic><topic>NUDOSIDADES RADICULARES</topic><topic>Phenotype</topic><topic>Plant Roots - microbiology</topic><topic>Plant Roots - ultrastructure</topic><topic>Plant Tumors - microbiology</topic><topic>Plants - microbiology</topic><topic>Plants - ultrastructure</topic><topic>Plants, Medicinal</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>REDUCCION</topic><topic>REDUCTION</topic><topic>Rhizobiaceae - genetics</topic><topic>Rhizobiaceae - ultrastructure</topic><topic>SECUENCIA NUCLEICA</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Deletion</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Species Specificity</topic><topic>Symbiosis - genetics</topic><topic>Transcription, Genetic</topic><topic>VIGNA UNGUICULATA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chun, J.Y. 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(University of California, Berkeley, Berkeley, CA.)</au><au>Sexton, G.L</au><au>Roth, L.E</au><au>Stacey, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a novel Bradyrhizobium japonicum gene involved in host-specific nitrogen fixation</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>176</volume><issue>21</issue><spage>6717</spage><epage>6729</epage><pages>6717-6729</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic development. This paper focuses on the little-understood genetics of host-specific nitrogen fixation. A deletion mutant of Bradyrhizobium japonicum, strain NAD163, was found to induce effective, nitrogen-fixing nodules on soybean and siratro plants but produced ineffective nodules on cowpea plants. Additional transposon and deletion mutants defined a small region that conferred this phenotype, and this region was sequenced to identify two putative open reading frames (ORFs). Data indicate that only one of these ORFs is detectable in bacteroids. This ORF was termed hsfA, with a predicted protein product of 11 kDa. The transcriptional start site of hsfA was determined and found to coincide with a predicted RpoN-dependent promoter. Microscopic studies of nodules induced by the wild type and hsfA mutants on cowpea and soybean plants indicate that the cowpea mutant nodules are slow to develop. The data indicate that hsfA appears to play a crucial role in bacteroid development on cowpea but does not appear to be essential for nitrogen fixation on the other hosts tested</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>7961425</pmid><doi>10.1128/JB.176.21.6717-6729.1994</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9193 |
| ispartof | Journal of Bacteriology, 1994-11, Vol.176 (21), p.6717-6729 |
| issn | 0021-9193 1098-5530 1067-8832 |
| language | eng |
| recordid | cdi_fao_agris_US9532700 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | ACETILENO ACETYLENE Amino Acid Sequence BACTERIA Bacterial Proteins - genetics Bacteriology Base Sequence BRADYRHIZOBIUM Bradyrhizobium japonicum COMPOSICION QUIMICA COMPOSITION CHIMIQUE EXPRESION GENICA EXPRESSION DES GENES Fabaceae - microbiology Fabaceae - ultrastructure FIJACION DEL NITROGENO FIXATION DE L'AZOTE FLORA MICROBIANA FLORE MICROBIENNE GENE GENES Genes, Bacterial - genetics Genetics GLYCINE MAX Molecular Sequence Data Mutagenesis Mutation Nitrogen Nitrogen Fixation - genetics NODOSITE RACINAIRE NUDOSIDADES RADICULARES Phenotype Plant Roots - microbiology Plant Roots - ultrastructure Plant Tumors - microbiology Plants - microbiology Plants - ultrastructure Plants, Medicinal PROTEINAS PROTEINE REDUCCION REDUCTION Rhizobiaceae - genetics Rhizobiaceae - ultrastructure SECUENCIA NUCLEICA Sequence Analysis, DNA Sequence Deletion SEQUENCE NUCLEIQUE Species Specificity Symbiosis - genetics Transcription, Genetic VIGNA UNGUICULATA |
| title | Identification and characterization of a novel Bradyrhizobium japonicum gene involved in host-specific nitrogen fixation |
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