Trehalose-6-P synthase is dispensable for growth on glucose but not for spore germination in Schizosaccharomyces pombe
Trehalose-6-P inhibits hexokinases in Saccharomyces cerevisiae (M.A. Blazquez, R. Lagunas, C. Gancedo, and J. M. Gancedo, FEBS Lett. 329:51-54, 1993), and disruption of the TPS1 gene (formerly named CIF1 or FDP1) encoding trehalose-6-P synthase prevents growth in glucose. We have found that the hexo...
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| creator | Blazquez, M.A Stucka, R Feldmann, H Gancedo, C |
| description | Trehalose-6-P inhibits hexokinases in Saccharomyces cerevisiae (M.A. Blazquez, R. Lagunas, C. Gancedo, and J. M. Gancedo, FEBS Lett. 329:51-54, 1993), and disruption of the TPS1 gene (formerly named CIF1 or FDP1) encoding trehalose-6-P synthase prevents growth in glucose. We have found that the hexokinase from Schizosaccharomyces pombe is not inhibited by trehalose-6-P even at a concentration of 3 mM. The highest internal concentration of trehalose-6-P that we measured in S. pombe was 0.75 mM after heat shock. We have isolated from S. pombe the tps1+ gene, which is homologous to the Saccharomyces cerevisiae TPS1 gene. The DNA sequence from tps1+ predicts a protein of 479 amino acids with 65% identity with the protein of S. cerevisiae. The tps1 gene expressed from its own promoter could complement the lack of trehalose-6-P synthase in S. cerevisiae tps1 mutants. The TPS1 gene from S. cerevisiae could also restore trehalose synthesis in S. pombe tps1 mutants. A chromosomal disruption of the tps1 gene in S. pombe did not have a noticeable effect on growth in glucose, in contrast with the disruption of TPS1 in S. cerevisiae. However, the disruption prevented germination of spores carrying it. The level of an RNA hybridizing with an internal probe of the tps1+ gene reached a maximum after 20 min of heat shock treatment. The results presented support the idea that trehalose-6-P plays a role in the control of glycolysis in S. cerevisiae but not in S. pombe and show that the trehalose pathway has different roles in the two yeast species |
| doi_str_mv | 10.1128/jb.176.13.3895-3902.1994 |
| format | Article |
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Lagunas, C. Gancedo, and J. M. Gancedo, FEBS Lett. 329:51-54, 1993), and disruption of the TPS1 gene (formerly named CIF1 or FDP1) encoding trehalose-6-P synthase prevents growth in glucose. We have found that the hexokinase from Schizosaccharomyces pombe is not inhibited by trehalose-6-P even at a concentration of 3 mM. The highest internal concentration of trehalose-6-P that we measured in S. pombe was 0.75 mM after heat shock. We have isolated from S. pombe the tps1+ gene, which is homologous to the Saccharomyces cerevisiae TPS1 gene. The DNA sequence from tps1+ predicts a protein of 479 amino acids with 65% identity with the protein of S. cerevisiae. The tps1 gene expressed from its own promoter could complement the lack of trehalose-6-P synthase in S. cerevisiae tps1 mutants. The TPS1 gene from S. cerevisiae could also restore trehalose synthesis in S. pombe tps1 mutants. A chromosomal disruption of the tps1 gene in S. pombe did not have a noticeable effect on growth in glucose, in contrast with the disruption of TPS1 in S. cerevisiae. However, the disruption prevented germination of spores carrying it. The level of an RNA hybridizing with an internal probe of the tps1+ gene reached a maximum after 20 min of heat shock treatment. The results presented support the idea that trehalose-6-P plays a role in the control of glycolysis in S. cerevisiae but not in S. pombe and show that the trehalose pathway has different roles in the two yeast species</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/jb.176.13.3895-3902.1994</identifier><identifier>PMID: 8021171</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Amino Acid Sequence ; AZUCARES FOSFATOS ; Bacteria ; Bacteriology ; Base Sequence ; Biochemistry ; Biological and medical sciences ; DNA Mutational Analysis ; ENDOMYCETALES ; ESPORAS ; ESTRES TERMICO ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; Gene Expression Regulation, Fungal ; GENES ; Genes, Fungal - genetics ; Genes. Genome ; GENETICA ; GENETIQUE ; GERMINACION ; GERMINATION ; GLICOSILTRANSFERASAS ; GLUCOSA ; GLUCOSE ; Glucose - metabolism ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; GLYCOSYLTRANSFERASE ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Hexokinase - drug effects ; Hexokinase - metabolism ; Hot Temperature ; METABOLISME DES GLUCIDES ; METABOLISMO DE CARBOHIDRATOS ; Microbiology ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis, Insertional ; MUTANT ; MUTANTES ; Mycology ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - genetics ; Schizosaccharomyces - enzymology ; Schizosaccharomyces - genetics ; Schizosaccharomyces - growth & development ; SECUENCIA NUCLEICA ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEIQUE ; SPORE ; Spores, Fungal - enzymology ; Spores, Fungal - genetics ; Spores, Fungal - growth & development ; STRESS THERMIQUE ; SUCRE PHOSPHATE ; Sugar Phosphates - biosynthesis ; Sugar Phosphates - pharmacology ; TREHALOSA ; TREHALOSE ; Trehalose - analogs & derivatives ; Trehalose - biosynthesis ; Trehalose - pharmacology ; Yeast</subject><ispartof>Journal of Bacteriology, 1994-07, Vol.176 (13), p.3895-3902</ispartof><rights>1994 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Jul 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c602t-6380f9cb351b4e20a1128ddbf379d7a7037293cfbdbb72c46d44bf3b9a641ec63</citedby><cites>FETCH-LOGICAL-c602t-6380f9cb351b4e20a1128ddbf379d7a7037293cfbdbb72c46d44bf3b9a641ec63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC205586/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC205586/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4167060$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8021171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blazquez, M.A</creatorcontrib><creatorcontrib>Stucka, R</creatorcontrib><creatorcontrib>Feldmann, H</creatorcontrib><creatorcontrib>Gancedo, C</creatorcontrib><title>Trehalose-6-P synthase is dispensable for growth on glucose but not for spore germination in Schizosaccharomyces pombe</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>Trehalose-6-P inhibits hexokinases in Saccharomyces cerevisiae (M.A. Blazquez, R. Lagunas, C. Gancedo, and J. M. Gancedo, FEBS Lett. 329:51-54, 1993), and disruption of the TPS1 gene (formerly named CIF1 or FDP1) encoding trehalose-6-P synthase prevents growth in glucose. We have found that the hexokinase from Schizosaccharomyces pombe is not inhibited by trehalose-6-P even at a concentration of 3 mM. The highest internal concentration of trehalose-6-P that we measured in S. pombe was 0.75 mM after heat shock. We have isolated from S. pombe the tps1+ gene, which is homologous to the Saccharomyces cerevisiae TPS1 gene. The DNA sequence from tps1+ predicts a protein of 479 amino acids with 65% identity with the protein of S. cerevisiae. The tps1 gene expressed from its own promoter could complement the lack of trehalose-6-P synthase in S. cerevisiae tps1 mutants. The TPS1 gene from S. cerevisiae could also restore trehalose synthesis in S. pombe tps1 mutants. A chromosomal disruption of the tps1 gene in S. pombe did not have a noticeable effect on growth in glucose, in contrast with the disruption of TPS1 in S. cerevisiae. However, the disruption prevented germination of spores carrying it. The level of an RNA hybridizing with an internal probe of the tps1+ gene reached a maximum after 20 min of heat shock treatment. The results presented support the idea that trehalose-6-P plays a role in the control of glycolysis in S. cerevisiae but not in S. pombe and show that the trehalose pathway has different roles in the two yeast species</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Amino Acid Sequence</subject><subject>AZUCARES FOSFATOS</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>DNA Mutational Analysis</subject><subject>ENDOMYCETALES</subject><subject>ESPORAS</subject><subject>ESTRES TERMICO</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>Gene Expression Regulation, Fungal</subject><subject>GENES</subject><subject>Genes, Fungal - genetics</subject><subject>Genes. Genome</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>GERMINACION</subject><subject>GERMINATION</subject><subject>GLICOSILTRANSFERASAS</subject><subject>GLUCOSA</subject><subject>GLUCOSE</subject><subject>Glucose - metabolism</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucosyltransferases - metabolism</subject><subject>GLYCOSYLTRANSFERASE</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Hexokinase - drug effects</subject><subject>Hexokinase - metabolism</subject><subject>Hot Temperature</subject><subject>METABOLISME DES GLUCIDES</subject><subject>METABOLISMO DE CARBOHIDRATOS</subject><subject>Microbiology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Mycology</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Schizosaccharomyces - enzymology</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - growth & development</subject><subject>SECUENCIA NUCLEICA</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>SPORE</subject><subject>Spores, Fungal - enzymology</subject><subject>Spores, Fungal - genetics</subject><subject>Spores, Fungal - growth & development</subject><subject>STRESS THERMIQUE</subject><subject>SUCRE PHOSPHATE</subject><subject>Sugar Phosphates - biosynthesis</subject><subject>Sugar Phosphates - pharmacology</subject><subject>TREHALOSA</subject><subject>TREHALOSE</subject><subject>Trehalose - analogs & derivatives</subject><subject>Trehalose - biosynthesis</subject><subject>Trehalose - pharmacology</subject><subject>Yeast</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1u1DAUhSMEKkPhBZCQLIS6S_Bf7HjBoqooIFUCqe3ash0n8SixBztpNTw9DjMaoKu7ON-59x6dogAIVgjh5uNWV4izCpGKNKIuiYC4QkLQZ8UGQdGUdU3g82IDIUalQIK8LF6ltIUQUVrjs-KsyQLiaFM83EU7qDEkW7LyB0h7Pw8qWeASaF3aWZ-UHi3oQgR9DI_zAIIH_biY7AB6mYEP8x817UK0oLdxcl7NLlPOg1szuF8hKWMGFcO0NzaBXZi0fV286NSY7JvjPC_urz_fXX0tb75_-XZ1eVMaBvFcMtLAThhNaqSpxVCt4dtWd4SLlisOCceCmE63WnNsKGspzaIWilFkDSPnxafD3t2iJ9sa6-eoRrmLblJxL4Ny8n_Fu0H24UFiWNfN6r84-mP4udg0y8klY8dReRuWJBEThFNGM_j-CbgNS_Q5m8SYw5pyUmeoOUAmhpSi7U6PICjXaHKrZe5VIiLXXuXaq1x7zdZ3_wY5GY9FZv3DUVfJqLGLyhuXThhFjEMG_745uH54dNFKlaYnVzP09gB1KkjVx7zn_lbUOQRtyG9IRMH6</recordid><startdate>19940701</startdate><enddate>19940701</enddate><creator>Blazquez, M.A</creator><creator>Stucka, R</creator><creator>Feldmann, H</creator><creator>Gancedo, C</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19940701</creationdate><title>Trehalose-6-P synthase is dispensable for growth on glucose but not for spore germination in Schizosaccharomyces pombe</title><author>Blazquez, M.A ; Stucka, R ; Feldmann, H ; Gancedo, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c602t-6380f9cb351b4e20a1128ddbf379d7a7037293cfbdbb72c46d44bf3b9a641ec63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Amino Acid Sequence</topic><topic>AZUCARES FOSFATOS</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>DNA Mutational Analysis</topic><topic>ENDOMYCETALES</topic><topic>ESPORAS</topic><topic>ESTRES TERMICO</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>Gene Expression Regulation, Fungal</topic><topic>GENES</topic><topic>Genes, Fungal - genetics</topic><topic>Genes. Genome</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>GERMINACION</topic><topic>GERMINATION</topic><topic>GLICOSILTRANSFERASAS</topic><topic>GLUCOSA</topic><topic>GLUCOSE</topic><topic>Glucose - metabolism</topic><topic>Glucosyltransferases - genetics</topic><topic>Glucosyltransferases - metabolism</topic><topic>GLYCOSYLTRANSFERASE</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Hexokinase - drug effects</topic><topic>Hexokinase - metabolism</topic><topic>Hot Temperature</topic><topic>METABOLISME DES GLUCIDES</topic><topic>METABOLISMO DE CARBOHIDRATOS</topic><topic>Microbiology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Mycology</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Schizosaccharomyces - enzymology</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - growth & development</topic><topic>SECUENCIA NUCLEICA</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>SPORE</topic><topic>Spores, Fungal - enzymology</topic><topic>Spores, Fungal - genetics</topic><topic>Spores, Fungal - growth & development</topic><topic>STRESS THERMIQUE</topic><topic>SUCRE PHOSPHATE</topic><topic>Sugar Phosphates - biosynthesis</topic><topic>Sugar Phosphates - pharmacology</topic><topic>TREHALOSA</topic><topic>TREHALOSE</topic><topic>Trehalose - analogs & derivatives</topic><topic>Trehalose - biosynthesis</topic><topic>Trehalose - pharmacology</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blazquez, M.A</creatorcontrib><creatorcontrib>Stucka, R</creatorcontrib><creatorcontrib>Feldmann, H</creatorcontrib><creatorcontrib>Gancedo, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blazquez, M.A</au><au>Stucka, R</au><au>Feldmann, H</au><au>Gancedo, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trehalose-6-P synthase is dispensable for growth on glucose but not for spore germination in Schizosaccharomyces pombe</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1994-07-01</date><risdate>1994</risdate><volume>176</volume><issue>13</issue><spage>3895</spage><epage>3902</epage><pages>3895-3902</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>Trehalose-6-P inhibits hexokinases in Saccharomyces cerevisiae (M.A. Blazquez, R. Lagunas, C. Gancedo, and J. M. Gancedo, FEBS Lett. 329:51-54, 1993), and disruption of the TPS1 gene (formerly named CIF1 or FDP1) encoding trehalose-6-P synthase prevents growth in glucose. We have found that the hexokinase from Schizosaccharomyces pombe is not inhibited by trehalose-6-P even at a concentration of 3 mM. The highest internal concentration of trehalose-6-P that we measured in S. pombe was 0.75 mM after heat shock. We have isolated from S. pombe the tps1+ gene, which is homologous to the Saccharomyces cerevisiae TPS1 gene. The DNA sequence from tps1+ predicts a protein of 479 amino acids with 65% identity with the protein of S. cerevisiae. The tps1 gene expressed from its own promoter could complement the lack of trehalose-6-P synthase in S. cerevisiae tps1 mutants. The TPS1 gene from S. cerevisiae could also restore trehalose synthesis in S. pombe tps1 mutants. A chromosomal disruption of the tps1 gene in S. pombe did not have a noticeable effect on growth in glucose, in contrast with the disruption of TPS1 in S. cerevisiae. However, the disruption prevented germination of spores carrying it. The level of an RNA hybridizing with an internal probe of the tps1+ gene reached a maximum after 20 min of heat shock treatment. The results presented support the idea that trehalose-6-P plays a role in the control of glycolysis in S. cerevisiae but not in S. pombe and show that the trehalose pathway has different roles in the two yeast species</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8021171</pmid><doi>10.1128/jb.176.13.3895-3902.1994</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Bacteriology, 1994-07, Vol.176 (13), p.3895-3902 |
| issn | 0021-9193 1098-5530 1067-8832 |
| language | eng |
| recordid | cdi_fao_agris_US9505448 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acid Sequence AZUCARES FOSFATOS Bacteria Bacteriology Base Sequence Biochemistry Biological and medical sciences DNA Mutational Analysis ENDOMYCETALES ESPORAS ESTRES TERMICO EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE Gene Expression Regulation, Fungal GENES Genes, Fungal - genetics Genes. Genome GENETICA GENETIQUE GERMINACION GERMINATION GLICOSILTRANSFERASAS GLUCOSA GLUCOSE Glucose - metabolism Glucosyltransferases - genetics Glucosyltransferases - metabolism GLYCOSYLTRANSFERASE Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hexokinase - drug effects Hexokinase - metabolism Hot Temperature METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Microbiology Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis, Insertional MUTANT MUTANTES Mycology SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Schizosaccharomyces - enzymology Schizosaccharomyces - genetics Schizosaccharomyces - growth & development SECUENCIA NUCLEICA Sequence Analysis, DNA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE SPORE Spores, Fungal - enzymology Spores, Fungal - genetics Spores, Fungal - growth & development STRESS THERMIQUE SUCRE PHOSPHATE Sugar Phosphates - biosynthesis Sugar Phosphates - pharmacology TREHALOSA TREHALOSE Trehalose - analogs & derivatives Trehalose - biosynthesis Trehalose - pharmacology Yeast |
| title | Trehalose-6-P synthase is dispensable for growth on glucose but not for spore germination in Schizosaccharomyces pombe |
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