Rhizobium leguminosarum CFN42 lipopolysaccharide antigenic changes induced by environmental conditions
Four monoclonal antibodies were raised against the lipopolysaccharide of Rhizobium leguminosarum bv. phaseoli CFN42 grown in tryptone and yeast extract. Two of these antibodies reacted relatively weakly with the lipopolysaccharide of bacteroids of this strain isolated from bean nodules. Growth ex pl...
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Veröffentlicht in: | Journal of Bacteriology 1992-04, Vol.174 (7), p.2222-2229 |
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description | Four monoclonal antibodies were raised against the lipopolysaccharide of Rhizobium leguminosarum bv. phaseoli CFN42 grown in tryptone and yeast extract. Two of these antibodies reacted relatively weakly with the lipopolysaccharide of bacteroids of this strain isolated from bean nodules. Growth ex planta of strain CFN42 at low pH, high temperature, low phosphate, or low oxygen concentration also eliminated binding of one or both of these antibodies. Lipopolysaccharide mobility on gel electrophoresis and reaction with other monoclonal antibodies and polyclonal antiserum indicated that the antigenic changes detected by these two antibodies did not represent major changes in lipopolysaccharide structure. The antigenic changes at low pH were dependent on growth of the bacteria but were independent of nitrogen and carbon sources and the rich or minimal quality of the medium. The Sym plasmid of this strain was not required for the changes induced ex planta. Analysis of bacterial mutants inferred to have truncated O-polysaccharides indicated that part, but not all, of the lipopolysaccharide O-polysaccharide portion was required for binding of these two antibodies. In addition, this analysis suggested that O-polysaccharide structures more distal to lipid A than the epitopes themselves were required for the modifications at low pH that prevented antibody binding. Two mutants were antigenically abnormal, even though they had abundant lipopolysaccharides of apparently normal size. One of these two mutants was constitutively unreactive toward three of the antibodies but indistinguishable from the wild type in symbiotic behavior. The other, whose bacteroids retained an epitope normally greatly diminished in bacteroids, was somewhat impaired in nodulation frequency and nodule development |
doi_str_mv | 10.1128/jb.174.7.2222-2229.1992 |
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(Yale University, New Haven, CT) ; Brewin, N.J ; Noel, K.D</creator><creatorcontrib>Tao, H. (Yale University, New Haven, CT) ; Brewin, N.J ; Noel, K.D</creatorcontrib><description>Four monoclonal antibodies were raised against the lipopolysaccharide of Rhizobium leguminosarum bv. phaseoli CFN42 grown in tryptone and yeast extract. Two of these antibodies reacted relatively weakly with the lipopolysaccharide of bacteroids of this strain isolated from bean nodules. Growth ex planta of strain CFN42 at low pH, high temperature, low phosphate, or low oxygen concentration also eliminated binding of one or both of these antibodies. Lipopolysaccharide mobility on gel electrophoresis and reaction with other monoclonal antibodies and polyclonal antiserum indicated that the antigenic changes detected by these two antibodies did not represent major changes in lipopolysaccharide structure. The antigenic changes at low pH were dependent on growth of the bacteria but were independent of nitrogen and carbon sources and the rich or minimal quality of the medium. The Sym plasmid of this strain was not required for the changes induced ex planta. Analysis of bacterial mutants inferred to have truncated O-polysaccharides indicated that part, but not all, of the lipopolysaccharide O-polysaccharide portion was required for binding of these two antibodies. In addition, this analysis suggested that O-polysaccharide structures more distal to lipid A than the epitopes themselves were required for the modifications at low pH that prevented antibody binding. Two mutants were antigenically abnormal, even though they had abundant lipopolysaccharides of apparently normal size. One of these two mutants was constitutively unreactive toward three of the antibodies but indistinguishable from the wild type in symbiotic behavior. The other, whose bacteroids retained an epitope normally greatly diminished in bacteroids, was somewhat impaired in nodulation frequency and nodule development</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/jb.174.7.2222-2229.1992</identifier><identifier>PMID: 1312998</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Antibodies, Bacterial - immunology ; Antibodies, Monoclonal - immunology ; ANTIGENE ; ANTIGENOS ; Antigens, Bacterial - immunology ; Bacteria ; Bacteriology ; Carbohydrate Sequence ; Cellular biology ; DNA Mutational Analysis ; DNA Transposable Elements ; DNA, Bacterial - genetics ; Environment ; FLORA MICROBIANA ; FLORE MICROBIENNE ; Flowers & plants ; FOSFATOS ; Hydrogen-Ion Concentration ; LIPOPOLISACARIDOS ; LIPOPOLYSACCHARIDE ; Lipopolysaccharides - genetics ; Lipopolysaccharides - immunology ; PHOSPHATE ; Plasmids ; Polysaccharides, Bacterial - immunology ; Rhizobium - immunology ; RHIZOBIUM LEGUMINOSARUM ; TEMPERATURA ; TEMPERATURE</subject><ispartof>Journal of Bacteriology, 1992-04, Vol.174 (7), p.2222-2229</ispartof><rights>Copyright American Society for Microbiology Apr 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-50e15a5f6e5433886bc78e77f4041714d44f14dc5db7bbb79b2d425bd0ccf64b3</citedby><cites>FETCH-LOGICAL-c568t-50e15a5f6e5433886bc78e77f4041714d44f14dc5db7bbb79b2d425bd0ccf64b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC205842/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC205842/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1312998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tao, H. (Yale University, New Haven, CT)</creatorcontrib><creatorcontrib>Brewin, N.J</creatorcontrib><creatorcontrib>Noel, K.D</creatorcontrib><title>Rhizobium leguminosarum CFN42 lipopolysaccharide antigenic changes induced by environmental conditions</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>Four monoclonal antibodies were raised against the lipopolysaccharide of Rhizobium leguminosarum bv. phaseoli CFN42 grown in tryptone and yeast extract. Two of these antibodies reacted relatively weakly with the lipopolysaccharide of bacteroids of this strain isolated from bean nodules. Growth ex planta of strain CFN42 at low pH, high temperature, low phosphate, or low oxygen concentration also eliminated binding of one or both of these antibodies. Lipopolysaccharide mobility on gel electrophoresis and reaction with other monoclonal antibodies and polyclonal antiserum indicated that the antigenic changes detected by these two antibodies did not represent major changes in lipopolysaccharide structure. The antigenic changes at low pH were dependent on growth of the bacteria but were independent of nitrogen and carbon sources and the rich or minimal quality of the medium. The Sym plasmid of this strain was not required for the changes induced ex planta. Analysis of bacterial mutants inferred to have truncated O-polysaccharides indicated that part, but not all, of the lipopolysaccharide O-polysaccharide portion was required for binding of these two antibodies. In addition, this analysis suggested that O-polysaccharide structures more distal to lipid A than the epitopes themselves were required for the modifications at low pH that prevented antibody binding. Two mutants were antigenically abnormal, even though they had abundant lipopolysaccharides of apparently normal size. One of these two mutants was constitutively unreactive toward three of the antibodies but indistinguishable from the wild type in symbiotic behavior. The other, whose bacteroids retained an epitope normally greatly diminished in bacteroids, was somewhat impaired in nodulation frequency and nodule development</description><subject>Antibodies, Bacterial - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Carbohydrate Sequence</subject><subject>Cellular biology</subject><subject>DNA Mutational Analysis</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - genetics</subject><subject>Environment</subject><subject>FLORA MICROBIANA</subject><subject>FLORE MICROBIENNE</subject><subject>Flowers & plants</subject><subject>FOSFATOS</subject><subject>Hydrogen-Ion Concentration</subject><subject>LIPOPOLISACARIDOS</subject><subject>LIPOPOLYSACCHARIDE</subject><subject>Lipopolysaccharides - genetics</subject><subject>Lipopolysaccharides - immunology</subject><subject>PHOSPHATE</subject><subject>Plasmids</subject><subject>Polysaccharides, Bacterial - immunology</subject><subject>Rhizobium - immunology</subject><subject>RHIZOBIUM LEGUMINOSARUM</subject><subject>TEMPERATURA</subject><subject>TEMPERATURE</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFv1SAUx4nRzLvpFzAxVh98awUKBR58MDfOmSyaqHsmQGnLTQtXaGfuPv246aJuLyPhnMD5_U8O_AF4g2CFEOYfdrpCjFSswnmVeYsKCYGfgA2CgpeU1vAp2ECIUSmQqJ-D05R2ECJCKD4BJ6hGWAi-Ad2Pwd0E7ZapGG2_TM6HpGI-bc-_EVyMbh_2YTwkZcygomttofzseuudKfKN720qnG8XY9tCHwrrr10MfrJ-VmNhgm_d7IJPL8CzTo3JvrzLZ-Dq_POv7UV5-f3L1-2ny9LQhs8lhRZRRbvGUlLXnDfaMG4Z6wgkiCHSEtLlaGirmdaaCY1bgqluoTFdQ3R9Bj6uffeLnmxr8hxRjXIf3aTiQQbl5P2Kd4Psw7XEkHKCs_79nT6G34tNs5xcMnYclbdhSZJh3jDYPA6iBjGKRZPBdw_AXViiz58gMc6tKGc8Q2yFTAwpRdv9nRhBefRb7rTMfksmj34fg5BHv7Py9f8P_qdbDc71t2t9cP3wx0UrVZrud8vMq5XpVJCqjy7Jq58CcV5jVt8CxSe8yA</recordid><startdate>19920401</startdate><enddate>19920401</enddate><creator>Tao, H. (Yale University, New Haven, CT)</creator><creator>Brewin, N.J</creator><creator>Noel, K.D</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920401</creationdate><title>Rhizobium leguminosarum CFN42 lipopolysaccharide antigenic changes induced by environmental conditions</title><author>Tao, H. (Yale University, New Haven, CT) ; Brewin, N.J ; Noel, K.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c568t-50e15a5f6e5433886bc78e77f4041714d44f14dc5db7bbb79b2d425bd0ccf64b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies, Bacterial - immunology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Carbohydrate Sequence</topic><topic>Cellular biology</topic><topic>DNA Mutational Analysis</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - genetics</topic><topic>Environment</topic><topic>FLORA MICROBIANA</topic><topic>FLORE MICROBIENNE</topic><topic>Flowers & plants</topic><topic>FOSFATOS</topic><topic>Hydrogen-Ion Concentration</topic><topic>LIPOPOLISACARIDOS</topic><topic>LIPOPOLYSACCHARIDE</topic><topic>Lipopolysaccharides - genetics</topic><topic>Lipopolysaccharides - immunology</topic><topic>PHOSPHATE</topic><topic>Plasmids</topic><topic>Polysaccharides, Bacterial - immunology</topic><topic>Rhizobium - immunology</topic><topic>RHIZOBIUM LEGUMINOSARUM</topic><topic>TEMPERATURA</topic><topic>TEMPERATURE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, H. (Yale University, New Haven, CT)</creatorcontrib><creatorcontrib>Brewin, N.J</creatorcontrib><creatorcontrib>Noel, K.D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, H. (Yale University, New Haven, CT)</au><au>Brewin, N.J</au><au>Noel, K.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhizobium leguminosarum CFN42 lipopolysaccharide antigenic changes induced by environmental conditions</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1992-04-01</date><risdate>1992</risdate><volume>174</volume><issue>7</issue><spage>2222</spage><epage>2229</epage><pages>2222-2229</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>Four monoclonal antibodies were raised against the lipopolysaccharide of Rhizobium leguminosarum bv. phaseoli CFN42 grown in tryptone and yeast extract. Two of these antibodies reacted relatively weakly with the lipopolysaccharide of bacteroids of this strain isolated from bean nodules. Growth ex planta of strain CFN42 at low pH, high temperature, low phosphate, or low oxygen concentration also eliminated binding of one or both of these antibodies. Lipopolysaccharide mobility on gel electrophoresis and reaction with other monoclonal antibodies and polyclonal antiserum indicated that the antigenic changes detected by these two antibodies did not represent major changes in lipopolysaccharide structure. The antigenic changes at low pH were dependent on growth of the bacteria but were independent of nitrogen and carbon sources and the rich or minimal quality of the medium. The Sym plasmid of this strain was not required for the changes induced ex planta. Analysis of bacterial mutants inferred to have truncated O-polysaccharides indicated that part, but not all, of the lipopolysaccharide O-polysaccharide portion was required for binding of these two antibodies. In addition, this analysis suggested that O-polysaccharide structures more distal to lipid A than the epitopes themselves were required for the modifications at low pH that prevented antibody binding. Two mutants were antigenically abnormal, even though they had abundant lipopolysaccharides of apparently normal size. One of these two mutants was constitutively unreactive toward three of the antibodies but indistinguishable from the wild type in symbiotic behavior. The other, whose bacteroids retained an epitope normally greatly diminished in bacteroids, was somewhat impaired in nodulation frequency and nodule development</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>1312998</pmid><doi>10.1128/jb.174.7.2222-2229.1992</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antibodies, Bacterial - immunology Antibodies, Monoclonal - immunology ANTIGENE ANTIGENOS Antigens, Bacterial - immunology Bacteria Bacteriology Carbohydrate Sequence Cellular biology DNA Mutational Analysis DNA Transposable Elements DNA, Bacterial - genetics Environment FLORA MICROBIANA FLORE MICROBIENNE Flowers & plants FOSFATOS Hydrogen-Ion Concentration LIPOPOLISACARIDOS LIPOPOLYSACCHARIDE Lipopolysaccharides - genetics Lipopolysaccharides - immunology PHOSPHATE Plasmids Polysaccharides, Bacterial - immunology Rhizobium - immunology RHIZOBIUM LEGUMINOSARUM TEMPERATURA TEMPERATURE |
title | Rhizobium leguminosarum CFN42 lipopolysaccharide antigenic changes induced by environmental conditions |
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