Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3' leads to 5'-exonuclease activities[F600;F200]; saccharomyces cerevisiae; dna polymerase; nucleases; purification; enzyme activity IND91026432

The yeast Saccharomyces cerevisiae catalytic DNA polymerase I 180-kDa subunit and the tightly associated 86-kDa polypeptide have been purified using immunoaffinity chromatography, permitting further characterization of the DNA polymerase activity of the DNA primase-DNA polymerase protein complex. Th...

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Veröffentlicht in:	The Journal of biological chemistry 1991-02, Vol.266 (5)
Hauptverfasser:	Brooke, R.G. (Indiana University, Bloomington, IN), Singhal, R, Hinkle, D.C, Dumas, L.B
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE NUCLEASAS NUCLEASE PURIFICACION PURIFICATION SACCHAROMYCES CEREVISIAE TRANSFERASAS TRANSFERASE
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