A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level
A locus in Pseudomonas solanacearum AW1 responsible for the hypersensitive response (HR) on tobacco was cloned by complementation in the tobacco-pathogenic strain P. solanearum NC252. The NC252 HR+ transconjugants lost pathogenicity on tobacco, indicating that the cloned locus could restrict the hos...
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| Veröffentlicht in: | Journal of Bacteriology 1990-09, Vol.172 (9), p.4836-4843 |
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| creator | Carney, B.F. (University of Georgia, Athens, GA) Denny, T.P |
| description | A locus in Pseudomonas solanacearum AW1 responsible for the hypersensitive response (HR) on tobacco was cloned by complementation in the tobacco-pathogenic strain P. solanearum NC252. The NC252 HR+ transconjugants lost pathogenicity on tobacco, indicating that the cloned locus could restrict the host range of NC252. Restriction enzyme mapping, transposon mutagenesis, and subcloning showed that, at most, 2.0 kilobases of the cloned DNA was required for NC252 transconjugants to elicit HR on tobacco. Site-directed insertional mutagenesis of the wild-type locus in strain AW1 to create AW1-31 eliminated HR activity on tobacco. However, AW1-31 retained pathogenicity on tomato and eggplant, confirming that this locus contains an avirulence gene, designated avrA. In contrast to the wild type, AW1-31 multiplied to almost the same extent as NC252 after infiltration into tobacco leaves. Nevertheless, AW1-31 did not wilt tobacco when stem inoculated, suggesting that additional factors condition host range. AW1 was HR+ on 27 N. tabacum cultivars, whereas AW1-31 was always HR-, strongly suggesting that avrA is specific at the host species level |
| doi_str_mv | 10.1128/jb.172.9.4836-4843.1990 |
| format | Article |
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(University of Georgia, Athens, GA) ; Denny, T.P</creator><creatorcontrib>Carney, B.F. (University of Georgia, Athens, GA) ; Denny, T.P</creatorcontrib><description>A locus in Pseudomonas solanacearum AW1 responsible for the hypersensitive response (HR) on tobacco was cloned by complementation in the tobacco-pathogenic strain P. solanearum NC252. The NC252 HR+ transconjugants lost pathogenicity on tobacco, indicating that the cloned locus could restrict the host range of NC252. Restriction enzyme mapping, transposon mutagenesis, and subcloning showed that, at most, 2.0 kilobases of the cloned DNA was required for NC252 transconjugants to elicit HR on tobacco. Site-directed insertional mutagenesis of the wild-type locus in strain AW1 to create AW1-31 eliminated HR activity on tobacco. However, AW1-31 retained pathogenicity on tomato and eggplant, confirming that this locus contains an avirulence gene, designated avrA. In contrast to the wild type, AW1-31 multiplied to almost the same extent as NC252 after infiltration into tobacco leaves. Nevertheless, AW1-31 did not wilt tobacco when stem inoculated, suggesting that additional factors condition host range. AW1 was HR+ on 27 N. tabacum cultivars, whereas AW1-31 was always HR-, strongly suggesting that avrA is specific at the host species level</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/jb.172.9.4836-4843.1990</identifier><identifier>PMID: 2203731</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteriology ; Biological and medical sciences ; Blotting, Southern ; CLONACION ; CLONAGE ; Cloning, Molecular ; Conjugation, Genetic ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Bacterial ; Genetics ; LOCI ; LOCUS ; Microbiology ; Mutation ; Nicotiana - microbiology ; NICOTIANA TABACUM ; PARASITISME ; PARASITISMO ; Plants - microbiology ; Plants, Toxic ; Plasmids ; PODER PATOGENO ; POUVOIR PATHOGENE ; PSEUDOMONAS ; Pseudomonas - genetics ; Pseudomonas - growth & development ; Pseudomonas - pathogenicity ; Restriction Mapping ; VIRULENCE ; Virulence - genetics ; VIRULENCIA</subject><ispartof>Journal of Bacteriology, 1990-09, Vol.172 (9), p.4836-4843</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c583t-6a86244e78fa30304b0e3805e56cc8e1dd3b1b10b2e3da23b09443c8d9efad583</citedby><cites>FETCH-LOGICAL-c583t-6a86244e78fa30304b0e3805e56cc8e1dd3b1b10b2e3da23b09443c8d9efad583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC213137/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC213137/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19448259$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2203731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carney, B.F. (University of Georgia, Athens, GA)</creatorcontrib><creatorcontrib>Denny, T.P</creatorcontrib><title>A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>A locus in Pseudomonas solanacearum AW1 responsible for the hypersensitive response (HR) on tobacco was cloned by complementation in the tobacco-pathogenic strain P. solanearum NC252. The NC252 HR+ transconjugants lost pathogenicity on tobacco, indicating that the cloned locus could restrict the host range of NC252. Restriction enzyme mapping, transposon mutagenesis, and subcloning showed that, at most, 2.0 kilobases of the cloned DNA was required for NC252 transconjugants to elicit HR on tobacco. Site-directed insertional mutagenesis of the wild-type locus in strain AW1 to create AW1-31 eliminated HR activity on tobacco. However, AW1-31 retained pathogenicity on tomato and eggplant, confirming that this locus contains an avirulence gene, designated avrA. In contrast to the wild type, AW1-31 multiplied to almost the same extent as NC252 after infiltration into tobacco leaves. Nevertheless, AW1-31 did not wilt tobacco when stem inoculated, suggesting that additional factors condition host range. AW1 was HR+ on 27 N. tabacum cultivars, whereas AW1-31 was always HR-, strongly suggesting that avrA is specific at the host species level</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>Cloning, Molecular</subject><subject>Conjugation, Genetic</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>Microbiology</subject><subject>Mutation</subject><subject>Nicotiana - microbiology</subject><subject>NICOTIANA TABACUM</subject><subject>PARASITISME</subject><subject>PARASITISMO</subject><subject>Plants - microbiology</subject><subject>Plants, Toxic</subject><subject>Plasmids</subject><subject>PODER PATOGENO</subject><subject>POUVOIR PATHOGENE</subject><subject>PSEUDOMONAS</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - growth & development</subject><subject>Pseudomonas - pathogenicity</subject><subject>Restriction Mapping</subject><subject>VIRULENCE</subject><subject>Virulence - genetics</subject><subject>VIRULENCIA</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUuP0zAUhS0EGkrhDyAhzAJ2KX4kjb2YxWjESxoBEszaunFuWleJXWynaHb8dFy1GpiVF-c75_reQ8hrzlacC_V-1614K1Z6VSu5rmpVyxXXmj0iC860qppGssdkwZjgleZaPiXPUtoxxuu6ERfkQggmW8kX5M8VtWPw2FM4uDiP6C3SDXqkQwwT_Z5w7sMUPCSawggeLEKcJ9pjxjg5j4k6b8O0h-w6N7p8R4OnX50N2RWaZujAFh4yzVuk25AyTXu0rhhHPOD4nDwZYEz44vwuye3HDz-vP1c33z59ub66qWyjZK7WoNairrFVA0gmWd0xlIo12KytVcj7Xna846wTKHsQsmO6rqVVvcYB-hKxJJen3P3cTdhb9DnCaPbRTRDvTABnHirebc0mHIzgkpdbLcm7sz-GXzOmbCaXLI7lJhjmZHjTqnWjmgK2J9DGkFLE4X4GZ-bYndl1pnRntDl2Z47dmWN3xfnq_y_e-85lFf3tWYdkYRwieOvSv_iysRKNLtybE7d1m-1vF9FAmh5OLczLEzNAMLCJJef2h2a1bpWQfwFsZboS</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Carney, B.F. (University of Georgia, Athens, GA)</creator><creator>Denny, T.P</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19900901</creationdate><title>A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level</title><author>Carney, B.F. (University of Georgia, Athens, GA) ; Denny, T.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c583t-6a86244e78fa30304b0e3805e56cc8e1dd3b1b10b2e3da23b09443c8d9efad583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>Cloning, Molecular</topic><topic>Conjugation, Genetic</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>Microbiology</topic><topic>Mutation</topic><topic>Nicotiana - microbiology</topic><topic>NICOTIANA TABACUM</topic><topic>PARASITISME</topic><topic>PARASITISMO</topic><topic>Plants - microbiology</topic><topic>Plants, Toxic</topic><topic>Plasmids</topic><topic>PODER PATOGENO</topic><topic>POUVOIR PATHOGENE</topic><topic>PSEUDOMONAS</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - growth & development</topic><topic>Pseudomonas - pathogenicity</topic><topic>Restriction Mapping</topic><topic>VIRULENCE</topic><topic>Virulence - genetics</topic><topic>VIRULENCIA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carney, B.F. (University of Georgia, Athens, GA)</creatorcontrib><creatorcontrib>Denny, T.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carney, B.F. (University of Georgia, Athens, GA)</au><au>Denny, T.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1990-09-01</date><risdate>1990</risdate><volume>172</volume><issue>9</issue><spage>4836</spage><epage>4843</epage><pages>4836-4843</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>A locus in Pseudomonas solanacearum AW1 responsible for the hypersensitive response (HR) on tobacco was cloned by complementation in the tobacco-pathogenic strain P. solanearum NC252. The NC252 HR+ transconjugants lost pathogenicity on tobacco, indicating that the cloned locus could restrict the host range of NC252. Restriction enzyme mapping, transposon mutagenesis, and subcloning showed that, at most, 2.0 kilobases of the cloned DNA was required for NC252 transconjugants to elicit HR on tobacco. Site-directed insertional mutagenesis of the wild-type locus in strain AW1 to create AW1-31 eliminated HR activity on tobacco. However, AW1-31 retained pathogenicity on tomato and eggplant, confirming that this locus contains an avirulence gene, designated avrA. In contrast to the wild type, AW1-31 multiplied to almost the same extent as NC252 after infiltration into tobacco leaves. Nevertheless, AW1-31 did not wilt tobacco when stem inoculated, suggesting that additional factors condition host range. AW1 was HR+ on 27 N. tabacum cultivars, whereas AW1-31 was always HR-, strongly suggesting that avrA is specific at the host species level</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>2203731</pmid><doi>10.1128/jb.172.9.4836-4843.1990</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteriology Biological and medical sciences Blotting, Southern CLONACION CLONAGE Cloning, Molecular Conjugation, Genetic DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genes, Bacterial Genetics LOCI LOCUS Microbiology Mutation Nicotiana - microbiology NICOTIANA TABACUM PARASITISME PARASITISMO Plants - microbiology Plants, Toxic Plasmids PODER PATOGENO POUVOIR PATHOGENE PSEUDOMONAS Pseudomonas - genetics Pseudomonas - growth & development Pseudomonas - pathogenicity Restriction Mapping VIRULENCE Virulence - genetics VIRULENCIA |
| title | A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level |
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