Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells
In rod photoreceptors, several phototransduction components display light-dependent translocation between cellular compartments. Notably, the G protein transducin translocates from rod outer segments to inner segments/spherules in bright light, but the functional consequences of translocation remain...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2013-07, Vol.110 (30), p.12468-12473 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Majumder, Anurima Pahlberg, Johan Boyd, Kimberly K. Kerov, Vasily Kolandaivelu, Saravanan Ramamurthy, Visvanathan Sampath, Alapakkam P. Artemyev, Nikolai O. |
| description | In rod photoreceptors, several phototransduction components display light-dependent translocation between cellular compartments. Notably, the G protein transducin translocates from rod outer segments to inner segments/spherules in bright light, but the functional consequences of translocation remain unclear. We generated transgenic mice where light-induced transducin translocation is impaired. These mice exhibited slow photoreceptor degeneration, which was prevented if they were dark-reared. Physiological recordings showed that control and transgenic rods and rod bipolar cells displayed similar sensitivity in darkness. After bright light exposure, control rods were more strongly desensitized than transgenic rods. However, in rod bipolar cells, this effect was reversed; transgenic rod bipolar cells were more strongly desensitized than control. This sensitivity reversal indicates that transducin translocation in rods enhances signaling to rod bipolar cells. The enhancement could not be explained by modulation of inner segment conductances or the voltage sensitivity of the synaptic Ca ²⁺ current, suggesting interactions of transducin with the synaptic machinery. |
| doi_str_mv | 10.1073/pnas.1222666110 |
| format | Article |
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Notably, the G protein transducin translocates from rod outer segments to inner segments/spherules in bright light, but the functional consequences of translocation remain unclear. We generated transgenic mice where light-induced transducin translocation is impaired. These mice exhibited slow photoreceptor degeneration, which was prevented if they were dark-reared. Physiological recordings showed that control and transgenic rods and rod bipolar cells displayed similar sensitivity in darkness. After bright light exposure, control rods were more strongly desensitized than transgenic rods. However, in rod bipolar cells, this effect was reversed; transgenic rod bipolar cells were more strongly desensitized than control. This sensitivity reversal indicates that transducin translocation in rods enhances signaling to rod bipolar cells. The enhancement could not be explained by modulation of inner segment conductances or the voltage sensitivity of the synaptic Ca ²⁺ current, suggesting interactions of transducin with the synaptic machinery.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1222666110</identifier><identifier>PMID: 23836670</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences</publisher><subject>Animals ; Biological and medical sciences ; Biological Sciences ; Cells ; Control rods ; Darkness ; Electric current ; Electric potential ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Light ; Mice ; Mice, Transgenic ; Neuroscience ; Photoreceptors ; Phototransduction ; Protein Transport ; Retina ; Retinal Degeneration - prevention & control ; Retinal Rod Photoreceptor Cells - cytology ; Retinal Rod Photoreceptor Cells - physiology ; Rodents ; Signal transduction ; Signal transmission ; Survival analysis ; Synaptic Transmission - physiology ; Transducin - metabolism ; Transducin - physiology ; Transgenic animals ; Vertebrates: nervous system and sense organs</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2013-07, Vol.110 (30), p.12468-12473</ispartof><rights>copyright © 1993-2008 National Academy of Sciences of the United States of America</rights><rights>2015 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Jul 23, 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-4d1acbc86bbce2f28f189b6e896107b9416f0b7e55629c3b1d3ae26a5532ec953</citedby><cites>FETCH-LOGICAL-c588t-4d1acbc86bbce2f28f189b6e896107b9416f0b7e55629c3b1d3ae26a5532ec953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/110/30.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42712614$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42712614$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27569207$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23836670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Majumder, Anurima</creatorcontrib><creatorcontrib>Pahlberg, Johan</creatorcontrib><creatorcontrib>Boyd, Kimberly K.</creatorcontrib><creatorcontrib>Kerov, Vasily</creatorcontrib><creatorcontrib>Kolandaivelu, Saravanan</creatorcontrib><creatorcontrib>Ramamurthy, Visvanathan</creatorcontrib><creatorcontrib>Sampath, Alapakkam P.</creatorcontrib><creatorcontrib>Artemyev, Nikolai O.</creatorcontrib><title>Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>In rod photoreceptors, several phototransduction components display light-dependent translocation between cellular compartments. Notably, the G protein transducin translocates from rod outer segments to inner segments/spherules in bright light, but the functional consequences of translocation remain unclear. We generated transgenic mice where light-induced transducin translocation is impaired. These mice exhibited slow photoreceptor degeneration, which was prevented if they were dark-reared. Physiological recordings showed that control and transgenic rods and rod bipolar cells displayed similar sensitivity in darkness. After bright light exposure, control rods were more strongly desensitized than transgenic rods. However, in rod bipolar cells, this effect was reversed; transgenic rod bipolar cells were more strongly desensitized than control. This sensitivity reversal indicates that transducin translocation in rods enhances signaling to rod bipolar cells. The enhancement could not be explained by modulation of inner segment conductances or the voltage sensitivity of the synaptic Ca ²⁺ current, suggesting interactions of transducin with the synaptic machinery.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences</subject><subject>Cells</subject><subject>Control rods</subject><subject>Darkness</subject><subject>Electric current</subject><subject>Electric potential</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Light</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Neuroscience</subject><subject>Photoreceptors</subject><subject>Phototransduction</subject><subject>Protein Transport</subject><subject>Retina</subject><subject>Retinal Degeneration - prevention & control</subject><subject>Retinal Rod Photoreceptor Cells - cytology</subject><subject>Retinal Rod Photoreceptor Cells - physiology</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>Signal transmission</subject><subject>Survival analysis</subject><subject>Synaptic Transmission - physiology</subject><subject>Transducin - metabolism</subject><subject>Transducin - physiology</subject><subject>Transgenic animals</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc9rFDEcxYModl09e1IHRPAybX7_uBSk-AsKHmzPIclk2iyzyZrMLPS_N8OsU_U0A9_Pey-PB8BrBM8RFOTiEE05RxhjzjlC8AnYIKhQy6mCT8EGQixaSTE9Ay9K2UEIFZPwOTjDRBLOBdyA4002sXSTC7EZ598hOTOGFBuX4piDnUZfmjE1OXVNmfIxHM3QmNg1Pt6b6OqxPERzGINb9PtQyizvc9rPolVswyENJjfOD0N5CZ71Zij-1em7BbdfPt9cfWuvf3z9fvXpunVMyrGlHTLOOsmtdR73WPZIKsu9VLzWt4oi3kMrPGMcK0cs6ojxmBvGCPZOMbIFl4vvYbJ73zlfO5lBH3LYm_ygkwn630sM9_ouHTURmEGqqsHHk0FOvyZfRl0LzhVM9GkqGlGEGSKQkYq-_w_dpSnHWm-mmKBScF6pi4VyOZWSfb8-BkE9b6rnTfXjplXx9u8OK_9nxAp8OAGmODP0dQYXyiMnGFe4Om9Bc-LmhDW25pIajSmXFXmzILsyprwyFAuEOaL1_m659yZpc5drzO1PDBGHEBFOqSC_AZupyo4</recordid><startdate>20130723</startdate><enddate>20130723</enddate><creator>Majumder, Anurima</creator><creator>Pahlberg, Johan</creator><creator>Boyd, Kimberly K.</creator><creator>Kerov, Vasily</creator><creator>Kolandaivelu, Saravanan</creator><creator>Ramamurthy, Visvanathan</creator><creator>Sampath, Alapakkam P.</creator><creator>Artemyev, Nikolai O.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130723</creationdate><title>Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells</title><author>Majumder, Anurima ; Pahlberg, Johan ; Boyd, Kimberly K. ; Kerov, Vasily ; Kolandaivelu, Saravanan ; Ramamurthy, Visvanathan ; Sampath, Alapakkam P. ; Artemyev, Nikolai O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-4d1acbc86bbce2f28f189b6e896107b9416f0b7e55629c3b1d3ae26a5532ec953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences</topic><topic>Cells</topic><topic>Control rods</topic><topic>Darkness</topic><topic>Electric current</topic><topic>Electric potential</topic><topic>Eye and associated structures. 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The enhancement could not be explained by modulation of inner segment conductances or the voltage sensitivity of the synaptic Ca ²⁺ current, suggesting interactions of transducin with the synaptic machinery.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences</pub><pmid>23836670</pmid><doi>10.1073/pnas.1222666110</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Biological Sciences Cells Control rods Darkness Electric current Electric potential Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology Glycoproteins Light Mice Mice, Transgenic Neuroscience Photoreceptors Phototransduction Protein Transport Retina Retinal Degeneration - prevention & control Retinal Rod Photoreceptor Cells - cytology Retinal Rod Photoreceptor Cells - physiology Rodents Signal transduction Signal transmission Survival analysis Synaptic Transmission - physiology Transducin - metabolism Transducin - physiology Transgenic animals Vertebrates: nervous system and sense organs |
| title | Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells |
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