Cloning and characterization of the pyruvate carboxylase from Sinorhizobium meliloti Rm 1021

The gene encoding pyruvate carboxylase (pyc) was isolated from a Sinorhizobium meliloti Rm1021 cosmid bank by complementation of a Rhizobium tropici pyc mutant. PYC-negative mutants of S. meliloti Rm1021 were isolated by transposon mutagenesis and were unable to grow with glucose or pyruvate as sole...

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Veröffentlicht in:	Archives of microbiology 2001, Vol.176 (5), p.355-363
Hauptverfasser:	Dunn, M.F, Araiza, G, Finan, T.M
Format:	Artikel
Sprache:	eng
Schlagworte:	acetyl coenzyme A alfalfa aspartic acid biotin biotinylation carbon gene fusion genes glucose in vivo studies molecular weight mutagenesis mutants pyruvate carboxylase pyruvic acid Rhizobium tropici Sinorhizobium meliloti transcription (genetics) transposons
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