Effect of sainfoin hay and pomegranate peel extracts on in vitro fermentation and protein degradation using the RUSITEC technique

Refat, B., Anele, U., He, Z. X., Bassiony, S. M., Abdel-Rahman, G. A. and Yang, W. Z. 2015. Effect of sainfoin hay and pomegranate peel extracts on in vitro fermentation and protein degradation using the RUSITEC technique. Can. J. Anim. Sci. 95: 417-423. The objectives of this study were to determine the effect of supplementation of sainfoin hay extract (SHE) and pomegranate peel extract (PPE) on in vitro rumen fermentation characteristics, protein metabolism and nutrient digestibility using rumen simulation technique (RUSITEC). The experiment was a completely randomized design with three treatments and four replications. Dietary treatments were: (1) control (10% barley silage and 90% concentrate), and control supplemented (2) with SHE (4.1 g catechin equivalent kg-1 dry matter; DM) or (3) with PPE (2.8 g tannic acid equivalents kg-1 DM). The experiment consisted of 10 d of adaptation and 7 d of data collection. Concentration of total volatile fatty acid (VFA) was lower (P