RAPD ANALYSIS OF A VARIANT OF BANANA (MUSA SP.) CV. GRANDE NAINE AND ITS PROP>AGATION VIA SHOOT TIP CULTURE
A morphological variant obtained from in vitro corm-derived plants of banana (Musa sp.) cv. Grande Naine (AAA) was evaluated up to harvest, and the genetic basis of variation was confirmed by the random amplified polymorphic DNA (RAPD). The corms formed during the multiplication phase of shoot tip-d...
Gespeichert in:
| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2006-03, Vol.42 (2), p.188-192 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 192 |
|---|---|
| container_issue | 2 |
| container_start_page | 188 |
| container_title | In vitro cellular & developmental biology. Plant |
| container_volume | 42 |
| creator | MARTIN, K. P. PACHATHUNDIKANDI, SUNEESH KUMAR ZHANG, C.-L. SLATER, A. MADASSERY, JOSEPH |
| description | A morphological variant obtained from in vitro corm-derived plants of banana (Musa sp.) cv. Grande Naine (AAA) was evaluated up to harvest, and the genetic basis of variation was confirmed by the random amplified polymorphic DNA (RAPD). The corms formed during the multiplication phase of shoot tip-derived cultures of the cv. Grande Naine grown on Murashige and Skoog (MS) medium enriched with 13.3 μMN6-benzyladenine (BA) developed numerous morphological variants after transfer to MS medium with 6.66 μM BA. The variant designated as CUDBT-B1, with distinct morphological features, was further evaluated. The morphological features of CUDBT-B1 were variegated leaf, pseudostem, bracts, ovary of the male flower and fruits, reduced height, decreased lamina length and breadth, and early flowering. These features were also manifested in the second-cycle progeny of CUDBT-B1. RAPD assay showed a marker DNA band of 1650 bp, and differential band intensity between the CUDBT-B1 and normal clone. CUDBT-B1 was multiplied using shoot tip culture, and the shoots were rooted on half-strength MS medium supplemented with 2.69 μM α-naphthaleneacetic acid. All plantlets showed variegated leaves under field conditions. |
| doi_str_mv | 10.1079/IVP2005736 |
| format | Article |
| fullrecord | <record><control><sourceid>fao_bioon</sourceid><recordid>TN_cdi_fao_agris_US201500203608</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US201500203608</sourcerecordid><originalsourceid>FETCH-LOGICAL-b578-a9974ef13be8464e481bea2e28652c476f0eeaf55e0eafce1dce47e088ad92033</originalsourceid><addsrcrecordid>eNpFkM1OwzAQhC0EEqVw4QXwsRxS1okdOxckk6atpeBEiROJU-S0Dip_Rc2Jt8dVkTjNrubblWYQuiUwJ8CTB9WWIQDjUXyGJoRyFoSxSM79DIwGjPL4El2N4xsAECB8gt4rWS6w1DJ_qVWNiyWWuJWVktoclyfvaIlnz00tcV3O73HazvGqknqRYS2VzvztAitT47Iqyke5kkYVGrfK4-uiMNioEqdNbpoqu0YXg_0Y3c2fTpFZZiZdB3mxUqnMg55xEdgk4dQNJOqdoDF1VJDe2dCFImbhxicYwDk7MObAy8aR7cZR7kAIu01CiKIpuju9Hey-s6-H3dg1dQiEAXg7BuGJ2Ynod_v9l-u-D7tPe_jpCHTHFrv_FqNfGbdYXA</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>RAPD ANALYSIS OF A VARIANT OF BANANA (MUSA SP.) CV. GRANDE NAINE AND ITS PROP>AGATION VIA SHOOT TIP CULTURE</title><source>BioOne Complete</source><source>Jstor Complete Legacy</source><source>SpringerLink Journals - AutoHoldings</source><creator>MARTIN, K. P. ; PACHATHUNDIKANDI, SUNEESH KUMAR ; ZHANG, C.-L. ; SLATER, A. ; MADASSERY, JOSEPH</creator><creatorcontrib>MARTIN, K. P. ; PACHATHUNDIKANDI, SUNEESH KUMAR ; ZHANG, C.-L. ; SLATER, A. ; MADASSERY, JOSEPH</creatorcontrib><description>A morphological variant obtained from in vitro corm-derived plants of banana (Musa sp.) cv. Grande Naine (AAA) was evaluated up to harvest, and the genetic basis of variation was confirmed by the random amplified polymorphic DNA (RAPD). The corms formed during the multiplication phase of shoot tip-derived cultures of the cv. Grande Naine grown on Murashige and Skoog (MS) medium enriched with 13.3 μMN6-benzyladenine (BA) developed numerous morphological variants after transfer to MS medium with 6.66 μM BA. The variant designated as CUDBT-B1, with distinct morphological features, was further evaluated. The morphological features of CUDBT-B1 were variegated leaf, pseudostem, bracts, ovary of the male flower and fruits, reduced height, decreased lamina length and breadth, and early flowering. These features were also manifested in the second-cycle progeny of CUDBT-B1. RAPD assay showed a marker DNA band of 1650 bp, and differential band intensity between the CUDBT-B1 and normal clone. CUDBT-B1 was multiplied using shoot tip culture, and the shoots were rooted on half-strength MS medium supplemented with 2.69 μM α-naphthaleneacetic acid. All plantlets showed variegated leaves under field conditions.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1079/IVP2005736</identifier><language>eng</language><publisher>Tissue Culture Association</publisher><subject>bananas ; bracts ; corm formation ; corms ; DNA ; flowering ; flowers ; fruits ; genetic markers ; harvesting ; internal transcribed spacers ; leaves ; males ; Musa ; plant ovary ; plantlets ; polymorphism ; progeny ; random amplified polymorphic DNA technique ; RAPD ; second-cycle ; shoot tip culture ; shoots ; variant</subject><ispartof>In vitro cellular & developmental biology. Plant, 2006-03, Vol.42 (2), p.188-192</ispartof><rights>Society for In Vitro Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1079/IVP2005736$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,778,782,26961,27907,27908,52346</link.rule.ids></links><search><creatorcontrib>MARTIN, K. P.</creatorcontrib><creatorcontrib>PACHATHUNDIKANDI, SUNEESH KUMAR</creatorcontrib><creatorcontrib>ZHANG, C.-L.</creatorcontrib><creatorcontrib>SLATER, A.</creatorcontrib><creatorcontrib>MADASSERY, JOSEPH</creatorcontrib><title>RAPD ANALYSIS OF A VARIANT OF BANANA (MUSA SP.) CV. GRANDE NAINE AND ITS PROP>AGATION VIA SHOOT TIP CULTURE</title><title>In vitro cellular & developmental biology. Plant</title><description>A morphological variant obtained from in vitro corm-derived plants of banana (Musa sp.) cv. Grande Naine (AAA) was evaluated up to harvest, and the genetic basis of variation was confirmed by the random amplified polymorphic DNA (RAPD). The corms formed during the multiplication phase of shoot tip-derived cultures of the cv. Grande Naine grown on Murashige and Skoog (MS) medium enriched with 13.3 μMN6-benzyladenine (BA) developed numerous morphological variants after transfer to MS medium with 6.66 μM BA. The variant designated as CUDBT-B1, with distinct morphological features, was further evaluated. The morphological features of CUDBT-B1 were variegated leaf, pseudostem, bracts, ovary of the male flower and fruits, reduced height, decreased lamina length and breadth, and early flowering. These features were also manifested in the second-cycle progeny of CUDBT-B1. RAPD assay showed a marker DNA band of 1650 bp, and differential band intensity between the CUDBT-B1 and normal clone. CUDBT-B1 was multiplied using shoot tip culture, and the shoots were rooted on half-strength MS medium supplemented with 2.69 μM α-naphthaleneacetic acid. All plantlets showed variegated leaves under field conditions.</description><subject>bananas</subject><subject>bracts</subject><subject>corm formation</subject><subject>corms</subject><subject>DNA</subject><subject>flowering</subject><subject>flowers</subject><subject>fruits</subject><subject>genetic markers</subject><subject>harvesting</subject><subject>internal transcribed spacers</subject><subject>leaves</subject><subject>males</subject><subject>Musa</subject><subject>plant ovary</subject><subject>plantlets</subject><subject>polymorphism</subject><subject>progeny</subject><subject>random amplified polymorphic DNA technique</subject><subject>RAPD</subject><subject>second-cycle</subject><subject>shoot tip culture</subject><subject>shoots</subject><subject>variant</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNpFkM1OwzAQhC0EEqVw4QXwsRxS1okdOxckk6atpeBEiROJU-S0Dip_Rc2Jt8dVkTjNrubblWYQuiUwJ8CTB9WWIQDjUXyGJoRyFoSxSM79DIwGjPL4El2N4xsAECB8gt4rWS6w1DJ_qVWNiyWWuJWVktoclyfvaIlnz00tcV3O73HazvGqknqRYS2VzvztAitT47Iqyke5kkYVGrfK4-uiMNioEqdNbpoqu0YXg_0Y3c2fTpFZZiZdB3mxUqnMg55xEdgk4dQNJOqdoDF1VJDe2dCFImbhxicYwDk7MObAy8aR7cZR7kAIu01CiKIpuju9Hey-s6-H3dg1dQiEAXg7BuGJ2Ynod_v9l-u-D7tPe_jpCHTHFrv_FqNfGbdYXA</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>MARTIN, K. P.</creator><creator>PACHATHUNDIKANDI, SUNEESH KUMAR</creator><creator>ZHANG, C.-L.</creator><creator>SLATER, A.</creator><creator>MADASSERY, JOSEPH</creator><general>Tissue Culture Association</general><scope>FBQ</scope></search><sort><creationdate>200603</creationdate><title>RAPD ANALYSIS OF A VARIANT OF BANANA (MUSA SP.) CV. GRANDE NAINE AND ITS PROP>AGATION VIA SHOOT TIP CULTURE</title><author>MARTIN, K. P. ; PACHATHUNDIKANDI, SUNEESH KUMAR ; ZHANG, C.-L. ; SLATER, A. ; MADASSERY, JOSEPH</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b578-a9974ef13be8464e481bea2e28652c476f0eeaf55e0eafce1dce47e088ad92033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>bananas</topic><topic>bracts</topic><topic>corm formation</topic><topic>corms</topic><topic>DNA</topic><topic>flowering</topic><topic>flowers</topic><topic>fruits</topic><topic>genetic markers</topic><topic>harvesting</topic><topic>internal transcribed spacers</topic><topic>leaves</topic><topic>males</topic><topic>Musa</topic><topic>plant ovary</topic><topic>plantlets</topic><topic>polymorphism</topic><topic>progeny</topic><topic>random amplified polymorphic DNA technique</topic><topic>RAPD</topic><topic>second-cycle</topic><topic>shoot tip culture</topic><topic>shoots</topic><topic>variant</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MARTIN, K. P.</creatorcontrib><creatorcontrib>PACHATHUNDIKANDI, SUNEESH KUMAR</creatorcontrib><creatorcontrib>ZHANG, C.-L.</creatorcontrib><creatorcontrib>SLATER, A.</creatorcontrib><creatorcontrib>MADASSERY, JOSEPH</creatorcontrib><collection>AGRIS</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MARTIN, K. P.</au><au>PACHATHUNDIKANDI, SUNEESH KUMAR</au><au>ZHANG, C.-L.</au><au>SLATER, A.</au><au>MADASSERY, JOSEPH</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RAPD ANALYSIS OF A VARIANT OF BANANA (MUSA SP.) CV. GRANDE NAINE AND ITS PROP>AGATION VIA SHOOT TIP CULTURE</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><date>2006-03</date><risdate>2006</risdate><volume>42</volume><issue>2</issue><spage>188</spage><epage>192</epage><pages>188-192</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>A morphological variant obtained from in vitro corm-derived plants of banana (Musa sp.) cv. Grande Naine (AAA) was evaluated up to harvest, and the genetic basis of variation was confirmed by the random amplified polymorphic DNA (RAPD). The corms formed during the multiplication phase of shoot tip-derived cultures of the cv. Grande Naine grown on Murashige and Skoog (MS) medium enriched with 13.3 μMN6-benzyladenine (BA) developed numerous morphological variants after transfer to MS medium with 6.66 μM BA. The variant designated as CUDBT-B1, with distinct morphological features, was further evaluated. The morphological features of CUDBT-B1 were variegated leaf, pseudostem, bracts, ovary of the male flower and fruits, reduced height, decreased lamina length and breadth, and early flowering. These features were also manifested in the second-cycle progeny of CUDBT-B1. RAPD assay showed a marker DNA band of 1650 bp, and differential band intensity between the CUDBT-B1 and normal clone. CUDBT-B1 was multiplied using shoot tip culture, and the shoots were rooted on half-strength MS medium supplemented with 2.69 μM α-naphthaleneacetic acid. All plantlets showed variegated leaves under field conditions.</abstract><pub>Tissue Culture Association</pub><doi>10.1079/IVP2005736</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1054-5476 |
| ispartof | In vitro cellular & developmental biology. Plant, 2006-03, Vol.42 (2), p.188-192 |
| issn | 1054-5476 1475-2689 |
| language | eng |
| recordid | cdi_fao_agris_US201500203608 |
| source | BioOne Complete; Jstor Complete Legacy; SpringerLink Journals - AutoHoldings |
| subjects | bananas bracts corm formation corms DNA flowering flowers fruits genetic markers harvesting internal transcribed spacers leaves males Musa plant ovary plantlets polymorphism progeny random amplified polymorphic DNA technique RAPD second-cycle shoot tip culture shoots variant |
| title | RAPD ANALYSIS OF A VARIANT OF BANANA (MUSA SP.) CV. GRANDE NAINE AND ITS PROP>AGATION VIA SHOOT TIP CULTURE |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T19%3A08%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_bioon&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RAPD%20ANALYSIS%20OF%20A%20VARIANT%20OF%20BANANA%20(MUSA%20SP.)%20CV.%20GRANDE%20NAINE%20AND%20ITS%20PROP%3EAGATION%20VIA%20SHOOT%20TIP%20CULTURE&rft.jtitle=In%20vitro%20cellular%20&%20developmental%20biology.%20Plant&rft.au=MARTIN,%20K.%20P.&rft.date=2006-03&rft.volume=42&rft.issue=2&rft.spage=188&rft.epage=192&rft.pages=188-192&rft.issn=1054-5476&rft.eissn=1475-2689&rft_id=info:doi/10.1079/IVP2005736&rft_dat=%3Cfao_bioon%3EUS201500203608%3C/fao_bioon%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |