Epithelial transforming growth factor β-activated kinase 1 (TAK1) is activated through two independent mechanisms and regulates reactive oxygen species

Dysregulation in cellular redox systems results in accumulation of reactive oxygen species (ROS), which are causally associated with a number of disease conditions. Transforming growth factor β-activated kinase 1 (TAK1) is a signaling intermediate of innate immune signaling pathways and is criticall...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2012-02, Vol.109 (9), p.3365-3370
Hauptverfasser: Omori, Emily, Inagaki, Maiko, Mishina, Yuji, Matsumoto, Kunihiro, Ninomiya-Tsuji, Jun
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Omori, Emily
Inagaki, Maiko
Mishina, Yuji
Matsumoto, Kunihiro
Ninomiya-Tsuji, Jun
description Dysregulation in cellular redox systems results in accumulation of reactive oxygen species (ROS), which are causally associated with a number of disease conditions. Transforming growth factor β-activated kinase 1 (TAK1) is a signaling intermediate of innate immune signaling pathways and is critically involved in the redox regulation in vivo. Ablation of TAK1 causes accumulation of ROS, resulting in epithelial cell death and inflammation. Here we determine the mechanism by which TAK1 kinase is activated in epithelial tissues. TAB1 and TAB2 are structurally unrelated TAK1 binding protein partners. TAB2 is known to mediate polyubiquitin chain-dependent TAK1 activation in innate immune signaling pathways, whereas the role of TAB1 is not defined. We found that epithelial-specific TAB1 and TAB2 double- but not TAB1 or TAB2 single-knockout mice phenocopied epithelial-specific TAK1 knockout mice. We demonstrate that phosphorylation-dependent basal activity of TAK1 is dependent on TAB1. Ablation of both TAB1 and TAB2 diminished the activity of TAK1 in vivo and causes accumulation of ROS in the epithelial tissues. These results demonstrate that epithelial TAK1 activity is regulated through two unique, TAB1-dependent basal and TAB2-mediated stimuli-dependent mechanisms.
doi_str_mv 10.1073/pnas.1116188109
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These results demonstrate that epithelial TAK1 activity is regulated through two unique, TAB1-dependent basal and TAB2-mediated stimuli-dependent mechanisms.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>22331902</pmid><doi>10.1073/pnas.1116188109</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Adaptor Proteins, Signal Transducing - deficiency
Adaptor Proteins, Signal Transducing - genetics
Adaptor Proteins, Signal Transducing - metabolism
Animals
Apoptosis
binding proteins
Biological Sciences
cell death
Enzyme Activation
Epidermis
Epidermis - enzymology
Epithelial cells
Epithelial Cells - enzymology
Inflammation
Intestinal mucosa
Intestinal Mucosa - enzymology
Intestines
Keratinocytes
Keratinocytes - enzymology
MAP Kinase Kinase Kinases - metabolism
Mice
Mice, Knockout
Oxidation-Reduction
Oxidative Stress
Phenotype
Phosphatases
Phosphorylation
Protein Processing, Post-Translational
Proteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Signal Transduction
Skin
TAK1 protein
transforming growth factors
title Epithelial transforming growth factor β-activated kinase 1 (TAK1) is activated through two independent mechanisms and regulates reactive oxygen species
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