Identrification and expression of two baculovirus gp37 genes
The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37...
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| Veröffentlicht in: | Journal of general virology 1999, Vol.80, p.1823-1831 |
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| container_title | Journal of general virology |
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| creator | Phanis, C.G Miller, D.P Cassar, S.C Tristem, M Thiem, S.M O'Reilly, D.R |
| description | The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct and well-separated clades. There was no evidence of recent gene transfer between the two groups. The gp37 genes also showed a distant similarity to bacterial cellulose- and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from consensus baculovirus late transcription start sites. MbMNPV gp37 was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37 is N-glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum. |
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Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct and well-separated clades. There was no evidence of recent gene transfer between the two groups. The gp37 genes also showed a distant similarity to bacterial cellulose- and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from consensus baculovirus late transcription start sites. MbMNPV gp37 was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37 is N-glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><language>eng</language><subject>amino acid sequences ; genbank/af108960 ; genbank/u38895 ; gene expression ; messenger RNA ; molecular sequence data ; Nucleopolyhedrovirus ; nucleotide sequences</subject><ispartof>Journal of general virology, 1999, Vol.80, p.1823-1831</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>Phanis, C.G</creatorcontrib><creatorcontrib>Miller, D.P</creatorcontrib><creatorcontrib>Cassar, S.C</creatorcontrib><creatorcontrib>Tristem, M</creatorcontrib><creatorcontrib>Thiem, S.M</creatorcontrib><creatorcontrib>O'Reilly, D.R</creatorcontrib><title>Identrification and expression of two baculovirus gp37 genes</title><title>Journal of general virology</title><description>The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct and well-separated clades. There was no evidence of recent gene transfer between the two groups. The gp37 genes also showed a distant similarity to bacterial cellulose- and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from consensus baculovirus late transcription start sites. MbMNPV gp37 was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37 is N-glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum.</description><subject>amino acid sequences</subject><subject>genbank/af108960</subject><subject>genbank/u38895</subject><subject>gene expression</subject><subject>messenger RNA</subject><subject>molecular sequence data</subject><subject>Nucleopolyhedrovirus</subject><subject>nucleotide sequences</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFyt0KwiAYgGGJgtbPNeQNDD51ToTOoqjj6ni4TYcxdPht1eVH0HlHLw-8M5KxopQ5B63nJAPgPGeCqSVZIT4AWFFIlZH9pbVhTN75xow-BmpCS-17SBbxy-jo-Iq0Ns3Ux6dPE9JuEIp2NljckIUzPdrtr2uyOx1vh3PuTKxMlzxW9ysHJoBrWWrQ4v_xASmDM8Q</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Phanis, C.G</creator><creator>Miller, D.P</creator><creator>Cassar, S.C</creator><creator>Tristem, M</creator><creator>Thiem, S.M</creator><creator>O'Reilly, D.R</creator><scope>FBQ</scope></search><sort><creationdate>1999</creationdate><title>Identrification and expression of two baculovirus gp37 genes</title><author>Phanis, C.G ; Miller, D.P ; Cassar, S.C ; Tristem, M ; Thiem, S.M ; O'Reilly, D.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_US2013029569093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>amino acid sequences</topic><topic>genbank/af108960</topic><topic>genbank/u38895</topic><topic>gene expression</topic><topic>messenger RNA</topic><topic>molecular sequence data</topic><topic>Nucleopolyhedrovirus</topic><topic>nucleotide sequences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Phanis, C.G</creatorcontrib><creatorcontrib>Miller, D.P</creatorcontrib><creatorcontrib>Cassar, S.C</creatorcontrib><creatorcontrib>Tristem, M</creatorcontrib><creatorcontrib>Thiem, S.M</creatorcontrib><creatorcontrib>O'Reilly, D.R</creatorcontrib><collection>AGRIS</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Phanis, C.G</au><au>Miller, D.P</au><au>Cassar, S.C</au><au>Tristem, M</au><au>Thiem, S.M</au><au>O'Reilly, D.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identrification and expression of two baculovirus gp37 genes</atitle><jtitle>Journal of general virology</jtitle><date>1999</date><risdate>1999</risdate><volume>80</volume><spage>1823</spage><epage>1831</epage><pages>1823-1831</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct and well-separated clades. There was no evidence of recent gene transfer between the two groups. The gp37 genes also showed a distant similarity to bacterial cellulose- and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from consensus baculovirus late transcription start sites. MbMNPV gp37 was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37 is N-glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum.</abstract></addata></record> |
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| ispartof | Journal of general virology, 1999, Vol.80, p.1823-1831 |
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| language | eng |
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| source | Microbiology Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | amino acid sequences genbank/af108960 genbank/u38895 gene expression messenger RNA molecular sequence data Nucleopolyhedrovirus nucleotide sequences |
| title | Identrification and expression of two baculovirus gp37 genes |
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