Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay
Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the...
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| Veröffentlicht in: | Genome 2000-08, Vol.43 (4), p.656-660 |
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| creator | Subramanian, V Gurtu, S Nageswara Rao, R.C Nigam, S.N |
| description | Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing random amplified polymorphic DNA (RAPD) assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits.Key words: Arachis hypogaea L., RAPD, DNA polymorphism, oligonucleotide, random primers. |
| doi_str_mv | 10.1139/g00-034 |
| format | Article |
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Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing random amplified polymorphic DNA (RAPD) assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits.Key words: Arachis hypogaea L., RAPD, DNA polymorphism, oligonucleotide, random primers.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g00-034</identifier><identifier>PMID: 10984178</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>Arachis hypogaea ; Deoxyribonucleic acid ; DNA ; Flowers & plants ; Genes, Plant ; Genetic Linkage ; Genetic Markers ; genetic polymorphism ; Genetics ; Genotype ; Groundnuts ; Nuts - genetics ; Polymorphism, Genetic ; random amplified polymorphic DNA technique ; Random Amplified Polymorphic DNA Technique - methods</subject><ispartof>Genome, 2000-08, Vol.43 (4), p.656-660</ispartof><rights>Copyright National Research Council of Canada Aug 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4394-b8eabf6927ccbcd9256fa7a148e653c0e37b9d7488e38a66556dbeaccd8d0dd13</citedby><cites>FETCH-LOGICAL-c4394-b8eabf6927ccbcd9256fa7a148e653c0e37b9d7488e38a66556dbeaccd8d0dd13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g00-034$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g00-034$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,780,784,2932,27924,27925,64428,65234</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10984178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Subramanian, V</creatorcontrib><creatorcontrib>Gurtu, S</creatorcontrib><creatorcontrib>Nageswara Rao, R.C</creatorcontrib><creatorcontrib>Nigam, S.N</creatorcontrib><title>Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay</title><title>Genome</title><addtitle>Génome</addtitle><description>Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. 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This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits.Key words: Arachis hypogaea L., RAPD, DNA polymorphism, oligonucleotide, random primers.</description><subject>Arachis hypogaea</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Flowers & plants</subject><subject>Genes, Plant</subject><subject>Genetic Linkage</subject><subject>Genetic Markers</subject><subject>genetic polymorphism</subject><subject>Genetics</subject><subject>Genotype</subject><subject>Groundnuts</subject><subject>Nuts - genetics</subject><subject>Polymorphism, Genetic</subject><subject>random amplified polymorphic DNA technique</subject><subject>Random Amplified Polymorphic DNA Technique - methods</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0VtrFDEYBuAgil1X8R9o8MKqMJrT5HC5tB4KRUXtdcgkmW3KTDJNZoT9943uYkURr3Lz5M335QXgMUavMabqzRahBlF2B6wwk6ihlOC7YIUkxQ0Rih-BB6VcIYQRVfg-OMJISYaFXIF45nycQx-smUOKMPXw9OMGTmnYjSlPl6GMMERol2EO383sHdzmtEQXlxkuJcQtzCa6NEIzTkONqeD2rv2Z9eLL5vPpS2hKMbuH4F5vhuIfHc41uHj39tvJh-b80_uzk815YxlVrOmkN13PFRHWdtYp0vLeCFN387ylFnkqOuUEk9JTaThvW-46b6x10iHnMF2D5_vcKafrxZdZj6FYPwwm-rQULQhhSrXqvxALjpUSssJnf8CrtORYl9CEoLYluE62Bsd7ZHMqJfteTzmMJu80RvpHUboWpWtRVT45xC3d6N1vbt_M7WAx2-yLN9le_lKHFD25vsJX_4Z_P_t0j3uTtNnmUPTFV4IwRaR-MuOM3gD8_7Jz</recordid><startdate>200008</startdate><enddate>200008</enddate><creator>Subramanian, V</creator><creator>Gurtu, S</creator><creator>Nageswara Rao, R.C</creator><creator>Nigam, S.N</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200008</creationdate><title>Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay</title><author>Subramanian, V ; Gurtu, S ; Nageswara Rao, R.C ; Nigam, S.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4394-b8eabf6927ccbcd9256fa7a148e653c0e37b9d7488e38a66556dbeaccd8d0dd13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Arachis hypogaea</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Flowers & plants</topic><topic>Genes, Plant</topic><topic>Genetic Linkage</topic><topic>Genetic Markers</topic><topic>genetic polymorphism</topic><topic>Genetics</topic><topic>Genotype</topic><topic>Groundnuts</topic><topic>Nuts - genetics</topic><topic>Polymorphism, Genetic</topic><topic>random amplified polymorphic DNA technique</topic><topic>Random Amplified Polymorphic DNA Technique - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Subramanian, V</creatorcontrib><creatorcontrib>Gurtu, S</creatorcontrib><creatorcontrib>Nageswara Rao, R.C</creatorcontrib><creatorcontrib>Nigam, S.N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Subramanian, V</au><au>Gurtu, S</au><au>Nageswara Rao, R.C</au><au>Nigam, S.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2000-08</date><risdate>2000</risdate><volume>43</volume><issue>4</issue><spage>656</spage><epage>660</epage><pages>656-660</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing random amplified polymorphic DNA (RAPD) assay with 48 oligonucleotide primers. Of the 48 oligonucleotide primers only 7 (14.6%) yielded polymorphic amplification products. The total number of bands from the 7 primers was 408, of which 27 were polymorphic. Detection of polymorphism in cultivated groundnut opens up the possibility of development of its molecular map by judicious selection of genotypes that show DNA polymorphism. This approach will be useful for developing marker-assisted selection tools for genetic enhancement of groundnut for desirable traits.Key words: Arachis hypogaea L., RAPD, DNA polymorphism, oligonucleotide, random primers.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>10984178</pmid><doi>10.1139/g00-034</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arachis hypogaea Deoxyribonucleic acid DNA Flowers & plants Genes, Plant Genetic Linkage Genetic Markers genetic polymorphism Genetics Genotype Groundnuts Nuts - genetics Polymorphism, Genetic random amplified polymorphic DNA technique Random Amplified Polymorphic DNA Technique - methods |
| title | Identification of DNA polymorphism in cultivated groundnut using random amplified polymorphic DNA (RAPD) assay |
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