Detection of Clostridium perfrigens alpha toxin by enzyme-linked immunosorbent assay
An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of Clostridium perfringens a toxin in intestinal contents of animals which have died of suspected C perfringens type A enterotoxaemia. The test can also be used for testing culture supernatants of C perfringens isolate...
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| Veröffentlicht in: | Research in veterinary science 1997, Vol.63 (1), p.101-102 |
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| creator | Naylor, R.D Martin, P.K Barker, L.T |
| description | An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of Clostridium perfringens a toxin in intestinal contents of animals which have died of suspected C perfringens type A enterotoxaemia. The test can also be used for testing culture supernatants of C perfringens isolates for the presence of a toxin. The test was sensitive and quantitative detecting toxin down to the 25ng level. The use of the ELISA for the detection of a toxin in conjunction with those for e toxin and beta toxin, allows the differential diagnosis of C perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C perfringens. |
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The test can also be used for testing culture supernatants of C perfringens isolates for the presence of a toxin. The test was sensitive and quantitative detecting toxin down to the 25ng level. The use of the ELISA for the detection of a toxin in conjunction with those for e toxin and beta toxin, allows the differential diagnosis of C perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C perfringens.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><language>eng</language><subject>bacterial toxins ; Clostridium perfringens ; detection ; digesta ; enzyme-linked immunosorbent assay</subject><ispartof>Research in veterinary science, 1997, Vol.63 (1), p.101-102</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>Naylor, R.D</creatorcontrib><creatorcontrib>Martin, P.K</creatorcontrib><creatorcontrib>Barker, L.T</creatorcontrib><title>Detection of Clostridium perfrigens alpha toxin by enzyme-linked immunosorbent assay</title><title>Research in veterinary science</title><description>An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of Clostridium perfringens a toxin in intestinal contents of animals which have died of suspected C perfringens type A enterotoxaemia. The test can also be used for testing culture supernatants of C perfringens isolates for the presence of a toxin. The test was sensitive and quantitative detecting toxin down to the 25ng level. The use of the ELISA for the detection of a toxin in conjunction with those for e toxin and beta toxin, allows the differential diagnosis of C perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C perfringens.</description><subject>bacterial toxins</subject><subject>Clostridium perfringens</subject><subject>detection</subject><subject>digesta</subject><subject>enzyme-linked immunosorbent assay</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFzrsOgjAUgOHGaCJensHzAiRtue6ocRdnU-SAVWhJT0nEp9fB3elfvuGfsUAkkQxlmoo5CziP4jCReb5kK6IH5zwWIgtYuUePN6-tAdtA0VnyTtd67GFA1zjdoiFQ3XBX4O1LG6gmQPOeegw7bZ5Yg-770ViyrkLjQRGpacMWjeoIt7-u2e54KItT2Ch7Va3TdL2cJRcR_w7JhGfRf_EBkyk9cg</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Naylor, R.D</creator><creator>Martin, P.K</creator><creator>Barker, L.T</creator><scope>FBQ</scope></search><sort><creationdate>1997</creationdate><title>Detection of Clostridium perfrigens alpha toxin by enzyme-linked immunosorbent assay</title><author>Naylor, R.D ; Martin, P.K ; Barker, L.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_US2013028825073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>bacterial toxins</topic><topic>Clostridium perfringens</topic><topic>detection</topic><topic>digesta</topic><topic>enzyme-linked immunosorbent assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naylor, R.D</creatorcontrib><creatorcontrib>Martin, P.K</creatorcontrib><creatorcontrib>Barker, L.T</creatorcontrib><collection>AGRIS</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naylor, R.D</au><au>Martin, P.K</au><au>Barker, L.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Clostridium perfrigens alpha toxin by enzyme-linked immunosorbent assay</atitle><jtitle>Research in veterinary science</jtitle><date>1997</date><risdate>1997</risdate><volume>63</volume><issue>1</issue><spage>101</spage><epage>102</epage><pages>101-102</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of Clostridium perfringens a toxin in intestinal contents of animals which have died of suspected C perfringens type A enterotoxaemia. The test can also be used for testing culture supernatants of C perfringens isolates for the presence of a toxin. The test was sensitive and quantitative detecting toxin down to the 25ng level. The use of the ELISA for the detection of a toxin in conjunction with those for e toxin and beta toxin, allows the differential diagnosis of C perfringens types A, B, C and D enterotoxaemias from samples of intestinal contents and the typing of cultures of C perfringens.</abstract></addata></record> |
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| ispartof | Research in veterinary science, 1997, Vol.63 (1), p.101-102 |
| issn | 0034-5288 1532-2661 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | bacterial toxins Clostridium perfringens detection digesta enzyme-linked immunosorbent assay |
| title | Detection of Clostridium perfrigens alpha toxin by enzyme-linked immunosorbent assay |
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