Translational regulation of a specific gene during oogenesis and embryogenesis of Drosophila
Polysomal and postpolysomal mRNAs were prepared from Drosophila egg chambers or embryos of different developmental stages. Cell-free translation of these mRNAs followed by two-dimensional gel electrophoresis of the products indicated the presence of a specific mRNA that appears to be translated (pol...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1983-06, Vol.80 (11), p.3359-3363 |
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Sprache: | eng |
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Zusammenfassung: | Polysomal and postpolysomal mRNAs were prepared from Drosophila egg chambers or embryos of different developmental stages. Cell-free translation of these mRNAs followed by two-dimensional gel electrophoresis of the products indicated the presence of a specific mRNA that appears to be translated (polysome-associated) during oogenesis. This mRNA, designated T1 mRNA, is selectively excluded from polysomes in 3-hr- and 5-hr-old embryos and is again translated in 18-hr-old embryos. A clone containing DNA complementary to T1 mRNA was selected from a library of recombinant DNA prepared from polyadenylylated ovary RNA. This clone was positively identified by hybrid-selected translation followed by two-dimensional gel electrophoresis and autoradiography. T1 mRNA is polyadenylylated and codes for a small, acidic protein. The cloned probe hybridizes to a unique site (2L-39CD) of the polytene chromosomes, very close to the histone genes. The results suggest that this mRNA is under specific translational regulation in contrast to a background of a large number of other abundant mRNAs that are translated at all developmental stages examined. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.80.11.3359 |