Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype
Chlamydia trachomatis is the leading cause of infectious blindness worldwide and is the most commonly reported pathogen causing sexually transmitted infections. Tarp (translocated actin recruiting phosphoprotein), a type III secreted effector that mediates actin nucleation, is central to C. trachoma...
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| Veröffentlicht in: | Infection and Immunity 2010-09, Vol.78 (9), p.3678-3688 |
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| creator | Lutter, Erika I Bonner, Christine Holland, Martin J Suchland, Robert J Stamm, Walter E Jewett, Travis J McClarty, Grant Hackstadt, Ted |
| description | Chlamydia trachomatis is the leading cause of infectious blindness worldwide and is the most commonly reported pathogen causing sexually transmitted infections. Tarp (translocated actin recruiting phosphoprotein), a type III secreted effector that mediates actin nucleation, is central to C. trachomatis infection. The phylogenetic analysis of tarP from reference strains as well as ocular, genital, and lymphogranuloma venereum (LGV) clinical isolates demonstrated an evolutionary relationship with disease phenotype, with LGV and ocular isolates branched into clades that were separate from the urogenital isolates. The sequence analysis of Tarp indicated a high degree of variability and identified trends within clinical groupings. Tarps from LGV strains contained the highest number of tyrosine-rich repeat regions (up to nine) and the fewest (two) predicted actin binding domains. The converse was noted for Tarp proteins from ocular isolates that contained up to four actin binding domains and as few as one tyrosine-rich repeat region. The results suggest that Tarp is among the few known genes to play a role in C. trachomatis adaptations to specific niches within the host. |
| doi_str_mv | 10.1128/IAI.00515-10 |
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Tarp (translocated actin recruiting phosphoprotein), a type III secreted effector that mediates actin nucleation, is central to C. trachomatis infection. The phylogenetic analysis of tarP from reference strains as well as ocular, genital, and lymphogranuloma venereum (LGV) clinical isolates demonstrated an evolutionary relationship with disease phenotype, with LGV and ocular isolates branched into clades that were separate from the urogenital isolates. The sequence analysis of Tarp indicated a high degree of variability and identified trends within clinical groupings. Tarps from LGV strains contained the highest number of tyrosine-rich repeat regions (up to nine) and the fewest (two) predicted actin binding domains. The converse was noted for Tarp proteins from ocular isolates that contained up to four actin binding domains and as few as one tyrosine-rich repeat region. The results suggest that Tarp is among the few known genes to play a role in C. trachomatis adaptations to specific niches within the host.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.00515-10</identifier><identifier>PMID: 20605986</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Actins - metabolism ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Chlamydia trachomatis ; Chlamydia trachomatis - classification ; Chlamydia trachomatis - genetics ; Female ; Humans ; Male ; Molecular Pathogenesis ; Phenotype ; Phylogeny ; Polymorphism, Single Nucleotide ; Porins - genetics ; Protein Structure, Tertiary ; Repetitive Sequences, Amino Acid</subject><ispartof>Infection and Immunity, 2010-09, Vol.78 (9), p.3678-3688</ispartof><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-dd3bffe40250589aca473806b02f3843dbb905420340aaa8b46d651683e7f7c63</citedby><cites>FETCH-LOGICAL-c427t-dd3bffe40250589aca473806b02f3843dbb905420340aaa8b46d651683e7f7c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2937449/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2937449/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20605986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lutter, Erika I</creatorcontrib><creatorcontrib>Bonner, Christine</creatorcontrib><creatorcontrib>Holland, Martin J</creatorcontrib><creatorcontrib>Suchland, Robert J</creatorcontrib><creatorcontrib>Stamm, Walter E</creatorcontrib><creatorcontrib>Jewett, Travis J</creatorcontrib><creatorcontrib>McClarty, Grant</creatorcontrib><creatorcontrib>Hackstadt, Ted</creatorcontrib><title>Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>Chlamydia trachomatis is the leading cause of infectious blindness worldwide and is the most commonly reported pathogen causing sexually transmitted infections. 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The results suggest that Tarp is among the few known genes to play a role in C. trachomatis adaptations to specific niches within the host.</description><subject>Actins - metabolism</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydia trachomatis - classification</subject><subject>Chlamydia trachomatis - genetics</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Molecular Pathogenesis</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Porins - genetics</subject><subject>Protein Structure, Tertiary</subject><subject>Repetitive Sequences, Amino Acid</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EotvCjTOEExdSxt_2pdIqorBSJSrRnjhYTuJsjJx4sbNU-9_jsqWCE6fRzPz09J4eQq8wnGNM1IfNenMOwDGvMTxBKwxa1ZwT8hStALCuNRfyBJ3m_L2sjDH1HJ0QEMC1Eiv07Xo8hLh1s1t8V61nGw7Z5yoOVTMGOx16b6sl2W6Mk13K48amXWXnvmpiSi6UW5yrO7-MVRP87DsbquvRzXE57NwL9GywIbuXD_MM3V5-vGk-11dfPm2a9VXdMSKXuu9pOwyOAeHAlbadZZIqEC2QgSpG-7bVwBkBysBaq1omesGxUNTJQXaCnqGLo-5u306u79xcHAezS36y6WCi9ebfz-xHs40_DdFUMqaLwLsHgRR_7F1ezORz50Kws4v7bCRnSnNG8f_JAkpKxL2p90eySzHn5IZHPxjMfXGmFGd-F1cuBX_9d4ZH-E9TBXh7BEa_He98csbmyfiSTSqjDRVSFebNkRlsNHabfDa3XwlgClgp4BToLwOUqMk</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Lutter, Erika I</creator><creator>Bonner, Christine</creator><creator>Holland, Martin J</creator><creator>Suchland, Robert J</creator><creator>Stamm, Walter E</creator><creator>Jewett, Travis J</creator><creator>McClarty, Grant</creator><creator>Hackstadt, Ted</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>20100901</creationdate><title>Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype</title><author>Lutter, Erika I ; 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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Actins - metabolism Bacterial Proteins - chemistry Bacterial Proteins - genetics Chlamydia trachomatis Chlamydia trachomatis - classification Chlamydia trachomatis - genetics Female Humans Male Molecular Pathogenesis Phenotype Phylogeny Polymorphism, Single Nucleotide Porins - genetics Protein Structure, Tertiary Repetitive Sequences, Amino Acid |
| title | Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype |
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