Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

To construct a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis, the genome of the industrial microorganism Streptomyces avermitilis was systematically deleted to remove nonessential genes. A region of more than 1.4 Mb was deleted stepwise from the...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2010-02, Vol.107 (6), p.2646-2651
Hauptverfasser:	Komatsu, Mamoru, Uchiyama, Takuma, Ōmura, Satoshi, Cane, David E, Ikeda, Haruo
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism Biological Sciences Biosynthesis Cellular metabolism Cephamycins Cephamycins - biosynthesis Chromosomes Epoxy Compounds - metabolism Gas Chromatography-Mass Spectrometry Gene Deletion Gene expression Gene Expression Regulation, Bacterial Genes Genes, Essential - genetics Genetic Engineering - methods Genetic vectors Genome, Bacterial - genetics Genomes Genomics Industrial Microbiology - methods Macrolides - metabolism Magnetic Resonance Spectroscopy Metabolism Metabolites Microorganisms Multigene Family - genetics Mutation Polyketides Sesquiterpenes - metabolism Streptomyces Streptomyces - genetics Streptomyces - metabolism Streptomycin - biosynthesis Transformation, Bacterial
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!