Influence of milk factors on riboflavin uptake by rat intestinal everted sac
The uptake of riboflavin was measured in Krebs' medium and a system consisting of riboflavin-free dialyzed milk (providing milk factors) equalized to Krebs' medium with respect to different salt concentration and pH. The final riboflavin concentration in the medium ranged between 0.1 and 0...
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| Veröffentlicht in: | Annals of nutrition and metabolism 1992, Vol.36 (5/6), p.279-282 |
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| container_title | Annals of nutrition and metabolism |
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| creator | Singh, Raghvendar Deodhar, A.D. |
| description | The uptake of riboflavin was measured in Krebs' medium and a system consisting of riboflavin-free dialyzed milk (providing milk factors) equalized to Krebs' medium with respect to different salt concentration and pH. The final riboflavin concentration in the medium ranged between 0.1 and 0.5 micromolar. Total riboflavin incorporation was greater in the milk system and the increase ranged between 120 and 134% in comparison with Krebs' medium (100%). The Michaelis-Menten constant (Km) and Vmax for labelled riboflavin uptake were calculated by Lineweaver-Burk plotting of data. Km value decreased from 1.23 micromolar for Krebs' medium to 0.75 micromolar in the presence of milk factor(s). Vmax values were 62 and 60 pmol/100 mg tissue/min for Krebs' and milk systems, respectively. It was concluded that milk factor(s) enhanced intestinal uptake of riboflavin. |
| doi_str_mv | 10.1159/000177730 |
| format | Article |
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The final riboflavin concentration in the medium ranged between 0.1 and 0.5 micromolar. Total riboflavin incorporation was greater in the milk system and the increase ranged between 120 and 134% in comparison with Krebs' medium (100%). The Michaelis-Menten constant (Km) and Vmax for labelled riboflavin uptake were calculated by Lineweaver-Burk plotting of data. Km value decreased from 1.23 micromolar for Krebs' medium to 0.75 micromolar in the presence of milk factor(s). Vmax values were 62 and 60 pmol/100 mg tissue/min for Krebs' and milk systems, respectively. It was concluded that milk factor(s) enhanced intestinal uptake of riboflavin.</description><identifier>ISSN: 0250-6807</identifier><identifier>EISSN: 1421-9697</identifier><identifier>DOI: 10.1159/000177730</identifier><identifier>PMID: 1492754</identifier><language>eng</language><publisher>Basel, Switzerland: S. 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Karger AG, Basel</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-2efec9ecf661c4f7ef61a99187720b5c53e9a37a7e37c2654f485d6275cc0e4a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/45099938$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/45099938$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,799,4010,4036,4037,23909,23910,25118,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4509222$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1492754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Raghvendar</creatorcontrib><creatorcontrib>Deodhar, A.D.</creatorcontrib><title>Influence of milk factors on riboflavin uptake by rat intestinal everted sac</title><title>Annals of nutrition and metabolism</title><addtitle>Ann Nutr Metab</addtitle><description>The uptake of riboflavin was measured in Krebs' medium and a system consisting of riboflavin-free dialyzed milk (providing milk factors) equalized to Krebs' medium with respect to different salt concentration and pH. The final riboflavin concentration in the medium ranged between 0.1 and 0.5 micromolar. Total riboflavin incorporation was greater in the milk system and the increase ranged between 120 and 134% in comparison with Krebs' medium (100%). The Michaelis-Menten constant (Km) and Vmax for labelled riboflavin uptake were calculated by Lineweaver-Burk plotting of data. Km value decreased from 1.23 micromolar for Krebs' medium to 0.75 micromolar in the presence of milk factor(s). Vmax values were 62 and 60 pmol/100 mg tissue/min for Krebs' and milk systems, respectively. It was concluded that milk factor(s) enhanced intestinal uptake of riboflavin.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>diet</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Intermediate and energetic metabolism</subject><subject>intestines</subject><subject>Intestines - metabolism</subject><subject>Jejunum - metabolism</subject><subject>Male</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Milk</subject><subject>Milk Proteins - metabolism</subject><subject>nutrient uptake</subject><subject>Original Paper</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>riboflavin</subject><subject>Riboflavin - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><subject>vitamin deficiencies</subject><issn>0250-6807</issn><issn>1421-9697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1LxDAQhoMoun4cvCsGFMFDNUmTpjku4heselDPZTY7kbrddk1aYf-9WbqsKJ5yeB5m3nlDyCFnl5wrc8UY41rrlG2QAZeCJyYzepMMmFAsyXKmd8huCB_RErlU22SbSyO0kgMyeqhd1WFtkTaOzspqSh3YtvGBNjX15bhxFXyVNe3mLUyRjhfUQ0vLusXQljVUFL_QtzihAew-2XJQBTxYvXvk7fbm9fo-GT3fPVwPR4mVTLaJQIfWoHVZxq10Gl3GwRieay3YWFmVooFUg8ZUW5Ep6WSuJlkMbC1DCekeOe_nzn3z2cUgxawMFqsKamy6UOhUKq6NieJFL1rfhODRFXNfzsAvCs6KZXPFurnoHq-GduMZTn7MvqrIz1YcgoXKeahtGdaaVMwIIaJ22mtT8O_o13z49NgvKuYTF62jf62_WT5C_IpfO-JVeeQnPXfQFPDuY463F8F4ujyGpzHtNyEGnHQ</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Singh, Raghvendar</creator><creator>Deodhar, A.D.</creator><general>S. Karger</general><general>Karger</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Influence of milk factors on riboflavin uptake by rat intestinal everted sac</title><author>Singh, Raghvendar ; Deodhar, A.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-2efec9ecf661c4f7ef61a99187720b5c53e9a37a7e37c2654f485d6275cc0e4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>diet</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Intermediate and energetic metabolism</topic><topic>intestines</topic><topic>Intestines - metabolism</topic><topic>Jejunum - metabolism</topic><topic>Male</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Milk</topic><topic>Milk Proteins - metabolism</topic><topic>nutrient uptake</topic><topic>Original Paper</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>riboflavin</topic><topic>Riboflavin - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><topic>vitamin deficiencies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Raghvendar</creatorcontrib><creatorcontrib>Deodhar, A.D.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of nutrition and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Raghvendar</au><au>Deodhar, A.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of milk factors on riboflavin uptake by rat intestinal everted sac</atitle><jtitle>Annals of nutrition and metabolism</jtitle><addtitle>Ann Nutr Metab</addtitle><date>1992</date><risdate>1992</risdate><volume>36</volume><issue>5/6</issue><spage>279</spage><epage>282</epage><pages>279-282</pages><issn>0250-6807</issn><eissn>1421-9697</eissn><abstract>The uptake of riboflavin was measured in Krebs' medium and a system consisting of riboflavin-free dialyzed milk (providing milk factors) equalized to Krebs' medium with respect to different salt concentration and pH. The final riboflavin concentration in the medium ranged between 0.1 and 0.5 micromolar. Total riboflavin incorporation was greater in the milk system and the increase ranged between 120 and 134% in comparison with Krebs' medium (100%). The Michaelis-Menten constant (Km) and Vmax for labelled riboflavin uptake were calculated by Lineweaver-Burk plotting of data. Km value decreased from 1.23 micromolar for Krebs' medium to 0.75 micromolar in the presence of milk factor(s). Vmax values were 62 and 60 pmol/100 mg tissue/min for Krebs' and milk systems, respectively. It was concluded that milk factor(s) enhanced intestinal uptake of riboflavin.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger</pub><pmid>1492754</pmid><doi>10.1159/000177730</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0250-6807 |
| ispartof | Annals of nutrition and metabolism, 1992, Vol.36 (5/6), p.279-282 |
| issn | 0250-6807 1421-9697 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Animals Biological and medical sciences diet Fundamental and applied biological sciences. Psychology Intermediate and energetic metabolism intestines Intestines - metabolism Jejunum - metabolism Male Metabolisms and neurohumoral controls Milk Milk Proteins - metabolism nutrient uptake Original Paper Rats Rats, Wistar riboflavin Riboflavin - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems vitamin deficiencies |
| title | Influence of milk factors on riboflavin uptake by rat intestinal everted sac |
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