Fluorescent and radioactive solid phase dideoxy sequencing of pcr rpoducts in microtitre plates
In this paper we describe a rapid method for the direct generation of DNA sequencing templates from phage or bacteria. Sequencing of these PCR products can be performed by radioactive and fluorescent methods. The non-radioactive method has been used to sequence a total of approximately 100kb of huma...
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| Veröffentlicht in: | DNA sequence 1991, Vol.1 (4), p.279-283 |
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| container_title | DNA sequence |
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| creator | Jones, D.S.C Schofield, J.P Vaudin, M |
| description | In this paper we describe a rapid method for the direct generation of DNA sequencing templates from phage or bacteria. Sequencing of these PCR products can be performed by radioactive and fluorescent methods. The non-radioactive method has been used to sequence a total of approximately 100kb of human DNA fragments generated by digestion with Hpall and subsequent cloning. The method depends on direct small scale amplification using a biotinylated primer, and the binding of the product to streptavidin coated magnetic beads. All the procedures are carried out in a microtitre plate thus facilitating the handling of large numbers of clones and has potential for automation. |
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Sequencing of these PCR products can be performed by radioactive and fluorescent methods. The non-radioactive method has been used to sequence a total of approximately 100kb of human DNA fragments generated by digestion with Hpall and subsequent cloning. The method depends on direct small scale amplification using a biotinylated primer, and the binding of the product to streptavidin coated magnetic beads. All the procedures are carried out in a microtitre plate thus facilitating the handling of large numbers of clones and has potential for automation.</description><identifier>ISSN: 1042-5179</identifier><identifier>EISSN: 1029-2365</identifier><language>eng</language><subject>biotin ; fluorescence ; polymerase chain reaction ; sequence analysis</subject><ispartof>DNA sequence, 1991, Vol.1 (4), p.279-283</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010</link.rule.ids></links><search><creatorcontrib>Jones, D.S.C</creatorcontrib><creatorcontrib>Schofield, J.P</creatorcontrib><creatorcontrib>Vaudin, M</creatorcontrib><title>Fluorescent and radioactive solid phase dideoxy sequencing of pcr rpoducts in microtitre plates</title><title>DNA sequence</title><description>In this paper we describe a rapid method for the direct generation of DNA sequencing templates from phage or bacteria. Sequencing of these PCR products can be performed by radioactive and fluorescent methods. The non-radioactive method has been used to sequence a total of approximately 100kb of human DNA fragments generated by digestion with Hpall and subsequent cloning. The method depends on direct small scale amplification using a biotinylated primer, and the binding of the product to streptavidin coated magnetic beads. All the procedures are carried out in a microtitre plate thus facilitating the handling of large numbers of clones and has potential for automation.</description><subject>biotin</subject><subject>fluorescence</subject><subject>polymerase chain reaction</subject><subject>sequence analysis</subject><issn>1042-5179</issn><issn>1029-2365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqFyssKwjAQQNEgCj6_wfmBQprah2tR3KvrEpJpHalJzaSify-Ce1f3LO5IzFKptonKinz89UYleVpup2LOfJNS5nlVzUR96AYfkA26CNpZCNqS1ybSE4F9Rxb6q2YESxb96w2MjwGdIdeCb6A3AULv7WAiAzm4kwk-UgwIfacj8lJMGt0xrn5diPVhf94dk0b7WreBuL6clEwzmZZFKZXM_h8fss1Baw</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Jones, D.S.C</creator><creator>Schofield, J.P</creator><creator>Vaudin, M</creator><scope>FBQ</scope></search><sort><creationdate>1991</creationdate><title>Fluorescent and radioactive solid phase dideoxy sequencing of pcr rpoducts in microtitre plates</title><author>Jones, D.S.C ; Schofield, J.P ; Vaudin, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_US2013017670203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>biotin</topic><topic>fluorescence</topic><topic>polymerase chain reaction</topic><topic>sequence analysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Jones, D.S.C</creatorcontrib><creatorcontrib>Schofield, J.P</creatorcontrib><creatorcontrib>Vaudin, M</creatorcontrib><collection>AGRIS</collection><jtitle>DNA sequence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, D.S.C</au><au>Schofield, J.P</au><au>Vaudin, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent and radioactive solid phase dideoxy sequencing of pcr rpoducts in microtitre plates</atitle><jtitle>DNA sequence</jtitle><date>1991</date><risdate>1991</risdate><volume>1</volume><issue>4</issue><spage>279</spage><epage>283</epage><pages>279-283</pages><issn>1042-5179</issn><eissn>1029-2365</eissn><abstract>In this paper we describe a rapid method for the direct generation of DNA sequencing templates from phage or bacteria. Sequencing of these PCR products can be performed by radioactive and fluorescent methods. The non-radioactive method has been used to sequence a total of approximately 100kb of human DNA fragments generated by digestion with Hpall and subsequent cloning. The method depends on direct small scale amplification using a biotinylated primer, and the binding of the product to streptavidin coated magnetic beads. All the procedures are carried out in a microtitre plate thus facilitating the handling of large numbers of clones and has potential for automation.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1042-5179 |
| ispartof | DNA sequence, 1991, Vol.1 (4), p.279-283 |
| issn | 1042-5179 1029-2365 |
| language | eng |
| recordid | cdi_fao_agris_US201301767020 |
| source | Taylor & Francis Journals Complete |
| subjects | biotin fluorescence polymerase chain reaction sequence analysis |
| title | Fluorescent and radioactive solid phase dideoxy sequencing of pcr rpoducts in microtitre plates |
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