Improved Thermostability and Acetic Acid Tolerance of Escherichia coli via Directed Evolution of Homoserine o-Succinyltransferase

In Escherichia coli, growth is limited at elevated temperatures mainly because of the instability of a single enzyme, homoserine o-succinyltransferase (MetA), the first enzyme in the methionine biosynthesis pathway. The metA gene from the thermophile Geobacillus kaustophilus cloned into the E. coli...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-12, Vol.74 (24), p.7660-7668
Hauptverfasser:	Mordukhova, Elena A, Lee, Hee-Soon, Pan, Jae-Gu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetic acid Acetic Acid - pharmacology Amino Acid Substitution - genetics Amino acids Anti-Bacterial Agents - pharmacology Bacillaceae - enzymology Bacillaceae - genetics Biological and medical sciences Biosynthetic Pathways Biotechnology Cloning DNA Mutational Analysis DNA, Bacterial - genetics Drug Tolerance E coli Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - physiology Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fundamental and applied biological sciences. Psychology Genes Heat-Shock Response High temperature Homoserine O-Succinyltransferase - genetics Homoserine O-Succinyltransferase - metabolism Methods. Procedures. Technologies Microbiology Mutagenesis Mutagenesis, Site-Directed Mutation Mutation, Missense Physiology and Biotechnology Protein engineering Proteins
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