Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials
Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resi...
Gespeichert in:
| Veröffentlicht in: | Journal of Clinical Microbiology 2008-09, Vol.46 (9), p.2842-2847 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2847 |
|---|---|
| container_issue | 9 |
| container_start_page | 2842 |
| container_title | Journal of Clinical Microbiology |
| container_volume | 46 |
| creator | Goering, Richard V Shawar, Ribhi M Scangarella, Nicole E O'Hara, F. Patrick Amrine-Madsen, Heather West, Joshua M Dalessandro, Marybeth Becker, Julie A Walsh, Stacey L Miller, Linda A van Horn, Stephanie F Thomas, Elizabeth S Twynholm, Monique E |
| description | Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types. |
| doi_str_mv | 10.1128/JCM.00521-08 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_fao_a</sourceid><recordid>TN_cdi_fao_agris_US201301535525</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69515968</sourcerecordid><originalsourceid>FETCH-LOGICAL-c462t-cc9b97fd9d6347b465a8f10580e855f158bd640ecf95856660bb0a46fa2515093</originalsourceid><addsrcrecordid>eNpVkUFv1DAQhS1ERZfCjTOEA5xIsRPba1-Q0KqUoq6Q2FbiZk0ce-PKiVM7Ae0f4HfjsqvSyoexNJ_fjN9D6BXBp4RU4uO31foUY1aREosnaEGwFCXn-OdTtMBYspKQenmMnqd0gzGhlLFn6JgITihndIH-rIM3evYQi7PRtaZ3wYftrgi2WJupc9p574byh0kuTTBMBQzto85mTtqMk2u8KTYTjN3OBx20nlMBczS5XKTgYTKpsDH0xbkPDfhild86nS9X0YFPL9CRzcW8PNQTdP3l7Gr1tbz8fn6x-nxZasqrqdRaNnJpW9nymi6b_AUQlmAmsBGMWcJE03KKjbaSCcazDU2DgXILFSMMy_oEfdrrjnPTm1abYYrg1RhdD3GnAjj1uDO4Tm3DL1UxypecZoH3B4EYbmeTJtW7bID3MJgwJ8VlHiS5yOCHPahjSCkaez-EYHUXnMrBqX_BKXyHv3642H_4kFQG3h0ASNk3G2HQLt1zFeb1Mp_Mvd1zndt2v100ClKvbnSvKFdSVYJWmXmzZywEBduYda43FSY1JqxmrGL1X4PBuHs</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69515968</pqid></control><display><type>article</type><title>Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Goering, Richard V ; Shawar, Ribhi M ; Scangarella, Nicole E ; O'Hara, F. Patrick ; Amrine-Madsen, Heather ; West, Joshua M ; Dalessandro, Marybeth ; Becker, Julie A ; Walsh, Stacey L ; Miller, Linda A ; van Horn, Stephanie F ; Thomas, Elizabeth S ; Twynholm, Monique E</creator><creatorcontrib>Goering, Richard V ; Shawar, Ribhi M ; Scangarella, Nicole E ; O'Hara, F. Patrick ; Amrine-Madsen, Heather ; West, Joshua M ; Dalessandro, Marybeth ; Becker, Julie A ; Walsh, Stacey L ; Miller, Linda A ; van Horn, Stephanie F ; Thomas, Elizabeth S ; Twynholm, Monique E</creatorcontrib><description>Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.00521-08</identifier><identifier>PMID: 18614654</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteriology ; Biological and medical sciences ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Humans ; India - epidemiology ; Methicillin Resistance - genetics ; Methicillin-Resistant Staphylococcus aureus - genetics ; Microbiology ; Miscellaneous ; Molecular Epidemiology ; Skin - microbiology ; South Africa - epidemiology ; Staphylococcal Infections - epidemiology ; Staphylococcal Infections - microbiology ; Staphylococcus aureus - genetics</subject><ispartof>Journal of Clinical Microbiology, 2008-09, Vol.46 (9), p.2842-2847</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright © 2008, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-cc9b97fd9d6347b465a8f10580e855f158bd640ecf95856660bb0a46fa2515093</citedby><cites>FETCH-LOGICAL-c462t-cc9b97fd9d6347b465a8f10580e855f158bd640ecf95856660bb0a46fa2515093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2546764/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2546764/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20637373$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18614654$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goering, Richard V</creatorcontrib><creatorcontrib>Shawar, Ribhi M</creatorcontrib><creatorcontrib>Scangarella, Nicole E</creatorcontrib><creatorcontrib>O'Hara, F. Patrick</creatorcontrib><creatorcontrib>Amrine-Madsen, Heather</creatorcontrib><creatorcontrib>West, Joshua M</creatorcontrib><creatorcontrib>Dalessandro, Marybeth</creatorcontrib><creatorcontrib>Becker, Julie A</creatorcontrib><creatorcontrib>Walsh, Stacey L</creatorcontrib><creatorcontrib>Miller, Linda A</creatorcontrib><creatorcontrib>van Horn, Stephanie F</creatorcontrib><creatorcontrib>Thomas, Elizabeth S</creatorcontrib><creatorcontrib>Twynholm, Monique E</creatorcontrib><title>Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>India - epidemiology</subject><subject>Methicillin Resistance - genetics</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Skin - microbiology</subject><subject>South Africa - epidemiology</subject><subject>Staphylococcal Infections - epidemiology</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus - genetics</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFv1DAQhS1ERZfCjTOEA5xIsRPba1-Q0KqUoq6Q2FbiZk0ce-PKiVM7Ae0f4HfjsqvSyoexNJ_fjN9D6BXBp4RU4uO31foUY1aREosnaEGwFCXn-OdTtMBYspKQenmMnqd0gzGhlLFn6JgITihndIH-rIM3evYQi7PRtaZ3wYftrgi2WJupc9p574byh0kuTTBMBQzto85mTtqMk2u8KTYTjN3OBx20nlMBczS5XKTgYTKpsDH0xbkPDfhild86nS9X0YFPL9CRzcW8PNQTdP3l7Gr1tbz8fn6x-nxZasqrqdRaNnJpW9nymi6b_AUQlmAmsBGMWcJE03KKjbaSCcazDU2DgXILFSMMy_oEfdrrjnPTm1abYYrg1RhdD3GnAjj1uDO4Tm3DL1UxypecZoH3B4EYbmeTJtW7bID3MJgwJ8VlHiS5yOCHPahjSCkaez-EYHUXnMrBqX_BKXyHv3642H_4kFQG3h0ASNk3G2HQLt1zFeb1Mp_Mvd1zndt2v100ClKvbnSvKFdSVYJWmXmzZywEBduYda43FSY1JqxmrGL1X4PBuHs</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Goering, Richard V</creator><creator>Shawar, Ribhi M</creator><creator>Scangarella, Nicole E</creator><creator>O'Hara, F. Patrick</creator><creator>Amrine-Madsen, Heather</creator><creator>West, Joshua M</creator><creator>Dalessandro, Marybeth</creator><creator>Becker, Julie A</creator><creator>Walsh, Stacey L</creator><creator>Miller, Linda A</creator><creator>van Horn, Stephanie F</creator><creator>Thomas, Elizabeth S</creator><creator>Twynholm, Monique E</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080901</creationdate><title>Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials</title><author>Goering, Richard V ; Shawar, Ribhi M ; Scangarella, Nicole E ; O'Hara, F. Patrick ; Amrine-Madsen, Heather ; West, Joshua M ; Dalessandro, Marybeth ; Becker, Julie A ; Walsh, Stacey L ; Miller, Linda A ; van Horn, Stephanie F ; Thomas, Elizabeth S ; Twynholm, Monique E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-cc9b97fd9d6347b465a8f10580e855f158bd640ecf95856660bb0a46fa2515093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>India - epidemiology</topic><topic>Methicillin Resistance - genetics</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Skin - microbiology</topic><topic>South Africa - epidemiology</topic><topic>Staphylococcal Infections - epidemiology</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goering, Richard V</creatorcontrib><creatorcontrib>Shawar, Ribhi M</creatorcontrib><creatorcontrib>Scangarella, Nicole E</creatorcontrib><creatorcontrib>O'Hara, F. Patrick</creatorcontrib><creatorcontrib>Amrine-Madsen, Heather</creatorcontrib><creatorcontrib>West, Joshua M</creatorcontrib><creatorcontrib>Dalessandro, Marybeth</creatorcontrib><creatorcontrib>Becker, Julie A</creatorcontrib><creatorcontrib>Walsh, Stacey L</creatorcontrib><creatorcontrib>Miller, Linda A</creatorcontrib><creatorcontrib>van Horn, Stephanie F</creatorcontrib><creatorcontrib>Thomas, Elizabeth S</creatorcontrib><creatorcontrib>Twynholm, Monique E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goering, Richard V</au><au>Shawar, Ribhi M</au><au>Scangarella, Nicole E</au><au>O'Hara, F. Patrick</au><au>Amrine-Madsen, Heather</au><au>West, Joshua M</au><au>Dalessandro, Marybeth</au><au>Becker, Julie A</au><au>Walsh, Stacey L</au><au>Miller, Linda A</au><au>van Horn, Stephanie F</au><au>Thomas, Elizabeth S</au><au>Twynholm, Monique E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>46</volume><issue>9</issue><spage>2842</spage><epage>2847</epage><pages>2842-2847</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>Determining the genetic characteristics of Staphylococcus aureus is important for better understanding of the global and dynamic epidemiology of this organism as we witness the emergence and spread of virulent and antibiotic-resistant clones. We genotyped 292 S. aureus isolates (105 methicillin resistant and 187 methicillin susceptible) using a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and SCCmec typing. In addition, S. aureus isolates were tested for the presence of the Panton-Valentine leukocidin (PVL) genes. Isolates were recovered from patients with uncomplicated skin infections in 10 different countries during five phase III global clinical trials of retapamulin, a new topical antibiotic agent. The most common methicillin-resistant clone had multilocus sequence type 8, pulsed-field type USA300, and SCCmec type IV and possessed the PVL genes. This clone was isolated exclusively in the United States. The most common PVL-positive, methicillin-susceptible clone had multilocus sequence type 121 and pulsed-field type USA1200. This clone was found primarily in South Africa and the Russian Federation. Other clones were found at lower frequencies and were limited in their geographic distribution. Overall, considerable genetic diversity was observed within multilocus sequence type clonal complexes and pulsed-field types.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>18614654</pmid><doi>10.1128/JCM.00521-08</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0095-1137 |
| ispartof | Journal of Clinical Microbiology, 2008-09, Vol.46 (9), p.2842-2847 |
| issn | 0095-1137 1098-660X |
| language | eng |
| recordid | cdi_fao_agris_US201301535525 |
| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Bacteriology Biological and medical sciences Epidemiology Fundamental and applied biological sciences. Psychology Humans India - epidemiology Methicillin Resistance - genetics Methicillin-Resistant Staphylococcus aureus - genetics Microbiology Miscellaneous Molecular Epidemiology Skin - microbiology South Africa - epidemiology Staphylococcal Infections - epidemiology Staphylococcal Infections - microbiology Staphylococcus aureus - genetics |
| title | Molecular Epidemiology of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Global Clinical Trials |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T02%3A31%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_fao_a&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20Epidemiology%20of%20Methicillin-Resistant%20and%20Methicillin-Susceptible%20Staphylococcus%20aureus%20Isolates%20from%20Global%20Clinical%20Trials&rft.jtitle=Journal%20of%20Clinical%20Microbiology&rft.au=Goering,%20Richard%20V&rft.date=2008-09-01&rft.volume=46&rft.issue=9&rft.spage=2842&rft.epage=2847&rft.pages=2842-2847&rft.issn=0095-1137&rft.eissn=1098-660X&rft.coden=JCMIDW&rft_id=info:doi/10.1128/JCM.00521-08&rft_dat=%3Cproquest_fao_a%3E69515968%3C/proquest_fao_a%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69515968&rft_id=info:pmid/18614654&rfr_iscdi=true |