potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity
The yeast Schwanniomyces occidentalis has a high affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to
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| Veröffentlicht in: | The EMBO journal 1995, Vol.14 (3), p.3021-3027 |
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| container_title | The EMBO journal |
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| creator | Banuelos, M.A Klein, R.D Alexander-Bowman, S.J Rodriguez-Navarro, A |
| description | The yeast Schwanniomyces occidentalis has a high affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to |
| format | Article |
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We have cloned the gene HAK1 of S. occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S. cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S. occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S. occidentalis HAK1 expresses in K+-limiting conditions. Our data indicate that in K+-starved cells the system encoded by HAK1 is the major K+ transporter of S. occidentalis.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><language>eng</language><subject>active transport ; amino acid sequences ; genbank/u22945 ; gene expression ; genetic transformation ; hak1 gene ; ion transport ; messenger RNA ; molecular sequence data ; nucleotide sequences ; potassium ; proteins ; rubidium ; Saccharomyces cerevisiae ; Saccharomycetales ; structural genes</subject><ispartof>The EMBO journal, 1995, Vol.14 (3), p.3021-3027</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>Banuelos, M.A</creatorcontrib><creatorcontrib>Klein, R.D</creatorcontrib><creatorcontrib>Alexander-Bowman, S.J</creatorcontrib><creatorcontrib>Rodriguez-Navarro, A</creatorcontrib><title>potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity</title><title>The EMBO journal</title><description>The yeast Schwanniomyces occidentalis has a high affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to <0.03 micromolar. We have cloned the gene HAK1 of S. occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S. cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S. occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S. occidentalis HAK1 expresses in K+-limiting conditions. Our data indicate that in K+-starved cells the system encoded by HAK1 is the major K+ transporter of S. occidentalis.</description><subject>active transport</subject><subject>amino acid sequences</subject><subject>genbank/u22945</subject><subject>gene expression</subject><subject>genetic transformation</subject><subject>hak1 gene</subject><subject>ion transport</subject><subject>messenger RNA</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>potassium</subject><subject>proteins</subject><subject>rubidium</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomycetales</subject><subject>structural genes</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFjE1KxEAQRhtRMP6cwbpAoDszycS1jAguR9dDUXbSJUkqdFWUHMMbO4p7V48P3vfOXBG2jS8rv6vPXeGrJpTb0N5fuivVd-993e5C4b5mMVTlZQTLOOks2WIG6cBShDWiGhwofeI0sYwrRQUh4rc4GQ6skGSUQXpZFEx-P8_LDLqqxfGnsldKMTMlRiAZGBIqICTu02lPdOpkNP6IQDgjsa037qLDQePtH6_d3eP-5eGp7FCO2GfW4-uh8mHjQ-3bumk2_xvfe5tUdg</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Banuelos, M.A</creator><creator>Klein, R.D</creator><creator>Alexander-Bowman, S.J</creator><creator>Rodriguez-Navarro, A</creator><scope>FBQ</scope></search><sort><creationdate>1995</creationdate><title>potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity</title><author>Banuelos, M.A ; Klein, R.D ; Alexander-Bowman, S.J ; Rodriguez-Navarro, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_US2013015085663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>active transport</topic><topic>amino acid sequences</topic><topic>genbank/u22945</topic><topic>gene expression</topic><topic>genetic transformation</topic><topic>hak1 gene</topic><topic>ion transport</topic><topic>messenger RNA</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>potassium</topic><topic>proteins</topic><topic>rubidium</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomycetales</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banuelos, M.A</creatorcontrib><creatorcontrib>Klein, R.D</creatorcontrib><creatorcontrib>Alexander-Bowman, S.J</creatorcontrib><creatorcontrib>Rodriguez-Navarro, A</creatorcontrib><collection>AGRIS</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banuelos, M.A</au><au>Klein, R.D</au><au>Alexander-Bowman, S.J</au><au>Rodriguez-Navarro, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity</atitle><jtitle>The EMBO journal</jtitle><date>1995</date><risdate>1995</risdate><volume>14</volume><issue>3</issue><spage>3021</spage><epage>3027</epage><pages>3021-3027</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><abstract>The yeast Schwanniomyces occidentalis has a high affinity K+ uptake system with a high concentrative capacity, which is able to deplete the external K+ to <0.03 micromolar. We have cloned the gene HAK1 of S. occidentalis which complements defective K+ uptake by trk1 and trk1 trk2 mutants of Saccharomyces cerevisiae. When HAK1 was expressed in a trk1 trk2 S. cerevisiae mutant, transport affinities for K+ and other alkali cations resembled those of S. occidentalis. The predicted amino acid sequence of the HAK1 protein shows significant homology with the hydrophobic region of the Kup transporter of Escherichia coli. In S. occidentalis HAK1 expresses in K+-limiting conditions. Our data indicate that in K+-starved cells the system encoded by HAK1 is the major K+ transporter of S. occidentalis.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0261-4189 |
| ispartof | The EMBO journal, 1995, Vol.14 (3), p.3021-3027 |
| issn | 0261-4189 1460-2075 |
| language | eng |
| recordid | cdi_fao_agris_US201301508566 |
| source | EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | active transport amino acid sequences genbank/u22945 gene expression genetic transformation hak1 gene ion transport messenger RNA molecular sequence data nucleotide sequences potassium proteins rubidium Saccharomyces cerevisiae Saccharomycetales structural genes |
| title | potassium transporter of the yeast Schwanniomyces occidentalis homologous to the Kup system of Escherichia coli has a high concentrative capacity |
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