High-level secretion of correctly processed beta-lactamase form Saccharomyces cerevisiae using a high-copy-number secretion vector

We have sought to obtain a convenient system for the high-level production of secreted proteins in yeast. With the aid of a secretion reporter cassette we examined the secretion of beta-lactamase (Bla) as a model protein and found the highest expression in Saccharomyces cerevisiae using a high-copy-...

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Veröffentlicht in:	Gene 1994, Vol.142 (1), p.113-117
Hauptverfasser:	Castelli, L.A, Mardon, C.J, Strike, P.M, Azad, A.A, Macreadie, I.G
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-lactamase gene expression genetic transformation plasmid vectors protein secretion reporter genes Saccharomyces cerevisiae secretion cassette secretion reporter cassette
Online-Zugang:	Volltext
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