Cloning and characterization of a cDNA encoding aspartate aminotransferase-P1 from Lupinus angustifolous root tips

A root tip cDNA library, constructed in the lambda Zap II expression vector, was immunoscreened with a monoclonal antibody raised against aspartate aminotransferase-P1 from Lupinus angustifolius L. var Uniharvest. One 1452-base pair clone was isolated. The encoded protein sequence had high homology...

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Veröffentlicht in:	Plant physiology (Bethesda) 1994, Vol.104 (2), p.417-423
Hauptverfasser:	Winefield, C.S, Reddington, B.D, Jones, W.T, Reynolds, P.H.S, Farnden, K.J.F
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acid sequences aspartate transaminase cloning cytosol DNA Escherichia coli genbank/m92094 gene expression gene transfer genetic code genetic techniques and protocols genetic transformation Lupinus angustifolius molecular sequence data mutants nucleotide sequences root tips Saccharomyces cerevisiae
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