nuclear-envelope protein and transcriptional repressor LAP2{szligbeta} interacts with HDAC3 at the nuclear periphery, and induces histone H4 deacetylation

Nuclear-envelope proteins have been implicated in diverse and fundamental cell functions, among them transcriptional regulation. Gene expression at the territory of the nuclear periphery is known to be repressed by epigenetic modifications such as histone deacetylation and methylation. However, the mechanism by which nuclear-envelope proteins are involved in such modifications is still obscure. We have previously shown that LAP2{szligbeta}, an integral nuclear-envelope protein that contains the chromatin-binding LEM domain, was able to repress the transcriptional activity of the E2F5-DP3 heterodimer. Here, we show that LAP2{szligbeta}'s repressive activity is more general, encompassing various E2F members as well as other transcription factors such as p53 and NF-[kappa]B. We further show that LAP2{szligbeta} interacts at the nuclear envelope with HDAC3, a class-I histone deacetylase, and that TSA (an HDAC inhibitor) abrogates LAP2{szligbeta}'s repressive activity. Finally, we show that LAP2{szligbeta} is capable of inducing histone-H4 deacetylation. Our data provide evidence for the existence of a previously unknown repressive complex, composed of an integral nuclear membrane protein and a histone modifier, at the nuclear periphery.