high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization
Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and fluorescence in situ hybridization (FISH), a high-resolution karyotype was established in the cucumber. C-banding showed distinct heterochromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromo...
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| Veröffentlicht in: | Genome 2005-06, Vol.48 (3), p.534-540 |
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| creator | Koo, D.H Choi, H.W Cho, J Hur, Y Bang, J.W |
| description | Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and fluorescence in situ hybridization (FISH), a high-resolution karyotype was established in the cucumber. C-banding showed distinct heterochromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromosomes. The C-banding patterns were also consistent with the morphology of 4'-6-diamino-2-phenylindole dihydrochloride (DAPI)-stained pachytene chromosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and localized on the mitotic metaphase and meiotic pachytene chromosomes. CsRP1 was detected on the pericentromeric heterochromatic regions of all chromosomes, except chromosome 1. CsRP2 was detected on 5 (chromosomes 1, 2, 3, 4, and 7) of 7 chromosomes. All homologous chromosome pairs could be distinguished by FISH using 2 RAPD markers. This is the first report on molecular karyotyping of mitotic and meiotic spreads of cucumber. |
| doi_str_mv | 10.1139/g04-128 |
| format | Article |
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'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization</title><source>NRC Research Press</source><source>MEDLINE</source><creator>Koo, D.H ; Choi, H.W ; Cho, J ; Hur, Y ; Bang, J.W</creator><creatorcontrib>Koo, D.H ; Choi, H.W ; Cho, J ; Hur, Y ; Bang, J.W</creatorcontrib><description>Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and fluorescence in situ hybridization (FISH), a high-resolution karyotype was established in the cucumber. C-banding showed distinct heterochromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromosomes. The C-banding patterns were also consistent with the morphology of 4'-6-diamino-2-phenylindole dihydrochloride (DAPI)-stained pachytene chromosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and localized on the mitotic metaphase and meiotic pachytene chromosomes. CsRP1 was detected on the pericentromeric heterochromatic regions of all chromosomes, except chromosome 1. CsRP2 was detected on 5 (chromosomes 1, 2, 3, 4, and 7) of 7 chromosomes. All homologous chromosome pairs could be distinguished by FISH using 2 RAPD markers. This is the first report on molecular karyotyping of mitotic and meiotic spreads of cucumber.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g04-128</identifier><identifier>PMID: 16121249</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>Base Sequence ; chromosome banding ; Chromosomes ; Cucumbers ; Cucumis sativus ; Cucumis sativus - genetics ; Deoxyribonucleic acid ; DNA ; DNA, Ribosomal - genetics ; Fluorescence ; fluorescence in situ hybridization ; Genetic Markers ; Genomics ; heterochromatin ; Hybridization ; In Situ Hybridization, Fluorescence ; Indoles ; Karyotypes ; Karyotyping ; meiosis ; Metaphase - genetics ; mitosis ; Molecular Sequence Data ; nucleotide sequences ; Pachytene Stage - genetics ; Random Amplified Polymorphic DNA Technique ; repetitive sequences ; Repetitive Sequences, Nucleic Acid ; Ribosomal DNA ; RNA, Ribosomal, 5S - genetics</subject><ispartof>Genome, 2005-06, Vol.48 (3), p.534-540</ispartof><rights>Copyright National Research Council of Canada Jun 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-2eedefeb0540e480a691e5017ff25c90cc855e379a5027b10538772ec43db1f13</citedby><cites>FETCH-LOGICAL-c493t-2eedefeb0540e480a691e5017ff25c90cc855e379a5027b10538772ec43db1f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g04-128$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g04-128$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,776,780,2919,27901,27902,64401,64979</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16121249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koo, D.H</creatorcontrib><creatorcontrib>Choi, H.W</creatorcontrib><creatorcontrib>Cho, J</creatorcontrib><creatorcontrib>Hur, Y</creatorcontrib><creatorcontrib>Bang, J.W</creatorcontrib><title>high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization</title><title>Genome</title><addtitle>Génome</addtitle><description>Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and fluorescence in situ hybridization (FISH), a high-resolution karyotype was established in the cucumber. C-banding showed distinct heterochromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromosomes. The C-banding patterns were also consistent with the morphology of 4'-6-diamino-2-phenylindole dihydrochloride (DAPI)-stained pachytene chromosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and localized on the mitotic metaphase and meiotic pachytene chromosomes. CsRP1 was detected on the pericentromeric heterochromatic regions of all chromosomes, except chromosome 1. CsRP2 was detected on 5 (chromosomes 1, 2, 3, 4, and 7) of 7 chromosomes. All homologous chromosome pairs could be distinguished by FISH using 2 RAPD markers. This is the first report on molecular karyotyping of mitotic and meiotic spreads of cucumber.</description><subject>Base Sequence</subject><subject>chromosome banding</subject><subject>Chromosomes</subject><subject>Cucumbers</subject><subject>Cucumis sativus</subject><subject>Cucumis sativus - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fluorescence</subject><subject>fluorescence in situ hybridization</subject><subject>Genetic Markers</subject><subject>Genomics</subject><subject>heterochromatin</subject><subject>Hybridization</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Indoles</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>meiosis</subject><subject>Metaphase - genetics</subject><subject>mitosis</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Pachytene Stage - genetics</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>repetitive sequences</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Ribosomal DNA</subject><subject>RNA, Ribosomal, 5S - genetics</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkl2L1DAUhoso7riK_0CDF47Kds1J-pFeLuMnDCjq4mVI09M2ayepSbtQ_4__0wwzuKCIVzmQ55y8532TJA-BngPw6mVHsxSYuJWsIBM05ZzB7WRFBYeUlVVxktwL4YpSoLyCu8kJFMCAZdUq-dmbrk89BjfMk3GWfFN-cdMyInEt0bOedzV68myzr0wgQU3meg5ke07WX42d4t3W2W79nHi8RjVgQ-qFbNJa2cbY7oyMSvfLhBaJsmpYgglnsWrIp4uPr1JlmtjQDrOLCjRajcRYEsw0k36pvWnMD7VXdT-506oh4IPjeZpcvnn9ZfMu3X54-35zsU11VvEpZYgNtljTPKMYfVBFBZhTKNuW5bqiWos8R15WKqesrIHmXJQlQ53xpoYW-Gny9DB39O77jGGScWeNw6AsujnIQuQgRJH9F4SyYCL6HcEnf4BXbvbRiSAZo5moiqyI0PoAae9C8NjK0ZtdDEIClft8ZcxXxnwj-eg4bq532Nxwx0BvhFmvo6eovO5_U8cpcmzaCL74N_j3s48PcKucVJ03QV5-ZnG9-KOgyEXBfwHj78UF</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Koo, D.H</creator><creator>Choi, H.W</creator><creator>Cho, J</creator><creator>Hur, Y</creator><creator>Bang, J.W</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization</title><author>Koo, D.H ; Choi, H.W ; Cho, J ; Hur, Y ; Bang, J.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-2eedefeb0540e480a691e5017ff25c90cc855e379a5027b10538772ec43db1f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Base Sequence</topic><topic>chromosome banding</topic><topic>Chromosomes</topic><topic>Cucumbers</topic><topic>Cucumis sativus</topic><topic>Cucumis sativus - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fluorescence</topic><topic>fluorescence in situ hybridization</topic><topic>Genetic Markers</topic><topic>Genomics</topic><topic>heterochromatin</topic><topic>Hybridization</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Indoles</topic><topic>Karyotypes</topic><topic>Karyotyping</topic><topic>meiosis</topic><topic>Metaphase - genetics</topic><topic>mitosis</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Pachytene Stage - genetics</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>repetitive sequences</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Ribosomal DNA</topic><topic>RNA, Ribosomal, 5S - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koo, D.H</creatorcontrib><creatorcontrib>Choi, H.W</creatorcontrib><creatorcontrib>Cho, J</creatorcontrib><creatorcontrib>Hur, Y</creatorcontrib><creatorcontrib>Bang, J.W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koo, D.H</au><au>Choi, H.W</au><au>Cho, J</au><au>Hur, Y</au><au>Bang, J.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>48</volume><issue>3</issue><spage>534</spage><epage>540</epage><pages>534-540</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and fluorescence in situ hybridization (FISH), a high-resolution karyotype was established in the cucumber. C-banding showed distinct heterochromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromosomes. The C-banding patterns were also consistent with the morphology of 4'-6-diamino-2-phenylindole dihydrochloride (DAPI)-stained pachytene chromosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and localized on the mitotic metaphase and meiotic pachytene chromosomes. CsRP1 was detected on the pericentromeric heterochromatic regions of all chromosomes, except chromosome 1. CsRP2 was detected on 5 (chromosomes 1, 2, 3, 4, and 7) of 7 chromosomes. All homologous chromosome pairs could be distinguished by FISH using 2 RAPD markers. This is the first report on molecular karyotyping of mitotic and meiotic spreads of cucumber.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>16121249</pmid><doi>10.1139/g04-128</doi><tpages>7</tpages></addata></record> |
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| subjects | Base Sequence chromosome banding Chromosomes Cucumbers Cucumis sativus Cucumis sativus - genetics Deoxyribonucleic acid DNA DNA, Ribosomal - genetics Fluorescence fluorescence in situ hybridization Genetic Markers Genomics heterochromatin Hybridization In Situ Hybridization, Fluorescence Indoles Karyotypes Karyotyping meiosis Metaphase - genetics mitosis Molecular Sequence Data nucleotide sequences Pachytene Stage - genetics Random Amplified Polymorphic DNA Technique repetitive sequences Repetitive Sequences, Nucleic Acid Ribosomal DNA RNA, Ribosomal, 5S - genetics |
| title | high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ hybridization |
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