Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)
The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos Aires from 1995 to 2001 was evaluated by partial nucleotide sequencing of the G gene and enzyme-linked immunosorbent assay analysis with anti-G monoclonal antibodies. Phylogenetic analyses sh...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2005-05, Vol.43 (5), p.2266-2273 |
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| container_title | Journal of Clinical Microbiology |
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| creator | Galiano, Monica C Palomo, Concepcion Videla, Cristina M Arbiza, Juan Melero, José A Carballal, Guadalupe |
| description | The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos Aires from 1995 to 2001 was evaluated by partial nucleotide sequencing of the G gene and enzyme-linked immunosorbent assay analysis with anti-G monoclonal antibodies. Phylogenetic analyses showed that 37 group A strains clustered into five genotypes, whereas 20 group B strains clustered into three genotypes. Group A showed more genetic variability than group B. A close correlation between genotypes and antigenic patterns was observed. Changes detected in the G protein of viruses from both groups included (i) amino acid substitutions and(ii) differences in protein length due to either changes in stop codon usage or sequence duplications. Three B strains from 1999 exhibited a duplication of 20 amino acids, while one B strain from 2001 had 2 amino acids duplicated. The comparison among Argentinean HRSV strains and viruses isolated in other geographical areas during different epidemics is discussed. |
| doi_str_mv | 10.1128/JCM.43.5.2266-2273.2005 |
| format | Article |
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Phylogenetic analyses showed that 37 group A strains clustered into five genotypes, whereas 20 group B strains clustered into three genotypes. Group A showed more genetic variability than group B. A close correlation between genotypes and antigenic patterns was observed. Changes detected in the G protein of viruses from both groups included (i) amino acid substitutions and(ii) differences in protein length due to either changes in stop codon usage or sequence duplications. Three B strains from 1999 exhibited a duplication of 20 amino acids, while one B strain from 2001 had 2 amino acids duplicated. The comparison among Argentinean HRSV strains and viruses isolated in other geographical areas during different epidemics is discussed.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.43.5.2266-2273.2005</identifier><identifier>PMID: 15872254</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Amino Acid Sequence ; Antigens, Viral - blood ; Argentina ; Base Sequence ; Biological and medical sciences ; Cell Line, Tumor ; Child, Preschool ; DNA Primers ; Fundamental and applied biological sciences. Psychology ; Human respiratory syncytial virus ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Phylogeny ; Respiratory Syncytial Virus Infections - diagnosis ; Respiratory Syncytial Virus, Human - classification ; Respiratory Syncytial Virus, Human - genetics ; Respiratory Syncytial Virus, Human - immunology ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Virology</subject><ispartof>Journal of Clinical Microbiology, 2005-05, Vol.43 (5), p.2266-2273</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-de974a6e7cba722fb2e305c8f1659662fa9ea056944ae6e8a6cb36a8a95d7923</citedby><cites>FETCH-LOGICAL-c592t-de974a6e7cba722fb2e305c8f1659662fa9ea056944ae6e8a6cb36a8a95d7923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153737/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153737/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16764983$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15872254$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Galiano, Monica C</creatorcontrib><creatorcontrib>Palomo, Concepcion</creatorcontrib><creatorcontrib>Videla, Cristina M</creatorcontrib><creatorcontrib>Arbiza, Juan</creatorcontrib><creatorcontrib>Melero, José A</creatorcontrib><creatorcontrib>Carballal, Guadalupe</creatorcontrib><title>Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos Aires from 1995 to 2001 was evaluated by partial nucleotide sequencing of the G gene and enzyme-linked immunosorbent assay analysis with anti-G monoclonal antibodies. Phylogenetic analyses showed that 37 group A strains clustered into five genotypes, whereas 20 group B strains clustered into three genotypes. Group A showed more genetic variability than group B. A close correlation between genotypes and antigenic patterns was observed. Changes detected in the G protein of viruses from both groups included (i) amino acid substitutions and(ii) differences in protein length due to either changes in stop codon usage or sequence duplications. Three B strains from 1999 exhibited a duplication of 20 amino acids, while one B strain from 2001 had 2 amino acids duplicated. The comparison among Argentinean HRSV strains and viruses isolated in other geographical areas during different epidemics is discussed.</description><subject>Amino Acid Sequence</subject><subject>Antigens, Viral - blood</subject><subject>Argentina</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Child, Preschool</subject><subject>DNA Primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human respiratory syncytial virus</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Respiratory Syncytial Virus Infections - diagnosis</subject><subject>Respiratory Syncytial Virus, Human - classification</subject><subject>Respiratory Syncytial Virus, Human - genetics</subject><subject>Respiratory Syncytial Virus, Human - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>Virology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9uEzEQxlcIREPgFag5gNpDgv-s7fWlUoggLSpCIqXiZk0cb-Jq1w72blCehZfFIRGBE_LBmvFvvpmRv6I4J3hMCK3efpx-GpdszMeUCjGiVLIxxZg_KgYEq2okBP72uBhgrPiIECbPimcpPWBMypLzp8UZ4ZWklJeD4ufMets5g8Av0cR3bmV9ju4hOli4xnU7FGp03bfg0RebNi5CF-IOzXfe7DoHDbp3sU_oYhZDv0lo8lvo3SW6SaGBzi5R2NqI5nZrPZoGn6zpO7e1OQMph8h5NIm5aec8oAuiFEddQHkZcvm8eFJDk-yL4z0s7j68v5tej24_z26mk9uR4Yp2o6VVsgRhpVlA3qpeUMswN1VNBFdC0BqUBcyFKkuwwlYgzIIJqEDxpVSUDYurg-ymX7R2afIsERq9ia6FuNMBnP73xbu1XoWtJoQzmc-weHMUiOF7b1OnW5eMbRrwNvRJCymVUKL6L0hkJSRne1AeQBNDStHWf6YhWO8NoLMBdMk013sD6L0B9N4AufLl38uc6o4_noHXRwCSgaaO4I1LJ05IUaqKZe7VgVu71fqHi1ZDavWDaU9tM3N-YGoIGlYx63ydU0xYNp6SpKLsF4_lzuc</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Galiano, Monica C</creator><creator>Palomo, Concepcion</creator><creator>Videla, Cristina M</creator><creator>Arbiza, Juan</creator><creator>Melero, José A</creator><creator>Carballal, Guadalupe</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050501</creationdate><title>Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)</title><author>Galiano, Monica C ; Palomo, Concepcion ; Videla, Cristina M ; Arbiza, Juan ; Melero, José A ; Carballal, Guadalupe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-de974a6e7cba722fb2e305c8f1659662fa9ea056944ae6e8a6cb36a8a95d7923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Antigens, Viral - blood</topic><topic>Argentina</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Child, Preschool</topic><topic>DNA Primers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human respiratory syncytial virus</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Respiratory Syncytial Virus Infections - diagnosis</topic><topic>Respiratory Syncytial Virus, Human - classification</topic><topic>Respiratory Syncytial Virus, Human - genetics</topic><topic>Respiratory Syncytial Virus, Human - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galiano, Monica C</creatorcontrib><creatorcontrib>Palomo, Concepcion</creatorcontrib><creatorcontrib>Videla, Cristina M</creatorcontrib><creatorcontrib>Arbiza, Juan</creatorcontrib><creatorcontrib>Melero, José A</creatorcontrib><creatorcontrib>Carballal, Guadalupe</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galiano, Monica C</au><au>Palomo, Concepcion</au><au>Videla, Cristina M</au><au>Arbiza, Juan</au><au>Melero, José A</au><au>Carballal, Guadalupe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001)</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>43</volume><issue>5</issue><spage>2266</spage><epage>2273</epage><pages>2266-2273</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos Aires from 1995 to 2001 was evaluated by partial nucleotide sequencing of the G gene and enzyme-linked immunosorbent assay analysis with anti-G monoclonal antibodies. Phylogenetic analyses showed that 37 group A strains clustered into five genotypes, whereas 20 group B strains clustered into three genotypes. Group A showed more genetic variability than group B. A close correlation between genotypes and antigenic patterns was observed. Changes detected in the G protein of viruses from both groups included (i) amino acid substitutions and(ii) differences in protein length due to either changes in stop codon usage or sequence duplications. Three B strains from 1999 exhibited a duplication of 20 amino acids, while one B strain from 2001 had 2 amino acids duplicated. The comparison among Argentinean HRSV strains and viruses isolated in other geographical areas during different epidemics is discussed.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>15872254</pmid><doi>10.1128/JCM.43.5.2266-2273.2005</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Antigens, Viral - blood Argentina Base Sequence Biological and medical sciences Cell Line, Tumor Child, Preschool DNA Primers Fundamental and applied biological sciences. Psychology Human respiratory syncytial virus Humans Infectious diseases Medical sciences Microbiology Miscellaneous Molecular Sequence Data Phylogeny Respiratory Syncytial Virus Infections - diagnosis Respiratory Syncytial Virus, Human - classification Respiratory Syncytial Virus, Human - genetics Respiratory Syncytial Virus, Human - immunology Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - genetics RNA, Viral - isolation & purification Virology |
| title | Genetic and Antigenic Variability of Human Respiratory Syncytial Virus (Groups A and B) Isolated over Seven Consecutive Seasons in Argentina (1995 to 2001) |
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