Development of Inducible Systems To Engineer Conditional Mutants of Essential Genes of Helicobacter pylori

The Escherichia coli-Helicobacter pylori shuttle vector pHeL2 was modified to introduce the inducible LacIq-pTac system of E. coli, in which the promoters were engineered to be under the control of H. pylori RNA polymerase. The amiE gene promoter of H. pylori was taken to constitutively express the...

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Veröffentlicht in:	Applied and Environmental Microbiology 2008-04, Vol.74 (7), p.2095-2102
Hauptverfasser:	Boneca, Ivo G, Ecobichon, Chantal, Chaput, Catherine, Mathieu, Aurélie, Guadagnini, Stéphanie, Prévost, Marie-Christine, Colland, Frédéric, Labigne, Agnès, de Reuse, Hilde
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriology Biological and medical sciences E coli Escherichia Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes Genes, Essential Genetic Engineering Genetic Vectors Genetics and Molecular Biology Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - physiology Microbiology Molecular Sequence Data Mutagenesis Mutation Promoter Regions, Genetic RNA polymerase
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