Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects

Evolving levels of resistance in insects to the bioinsecticide Bacillus thuringiensis (Bt) can be dramatically reduced through the genetic engineering of chloroplasts in plants. When transgenic tobacco leaves expressing Cry2Aa2 protoxin in chloroplasts were fed to susceptible, Cry1A-resistant (20,00...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1999-03, Vol.96 (5), p.1840-1845
Hauptverfasser:	Kota, M. (Auburn University, AL.), Daniell, H, Varma, S, Garczynski, S.F, Gould, F, Moar, W.J
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Animals BACILLUS THURINGIENSIS Bacillus thuringiensis - genetics Bacillus thuringiensis Toxins Bacterial Proteins - biosynthesis Bacterial Proteins - genetics BACTERIAL TOXINS Bioassay BIOASSAYS Biological Assay Biological Sciences CHLOROPLAST DNA Chloroplasts Chloroplasts - physiology CRYSTAL PROTEINS DNA Endotoxins - biosynthesis Endotoxins - genetics ENSAYO BIOLOGICO EXPRESION GENICA EXPRESSION DES GENES FEUILLE GENE GENE EXPRESSION GENE TRANSFER GENES Genetic engineering GENETIC TRANSFORMATION Genomes HELICOVERPA ZEA HELIOTHIS VIRESCENS Hemolysin Proteins HOJAS Insect genetics INSECTICIDAL PROPERTIES INSECTICIDAS INSECTICIDE INSECTICIDES LARVAE LARVAS LARVE LEAVES Mortality Moths Nicotiana - genetics Nicotiana - physiology NICOTIANA TABACUM Noctuidae Pest Control, Biological PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants Plants, Genetically Modified Plants, Toxic Polymerase Chain Reaction PROTEINAS PROTEINE PROTEINS Recombinant Proteins - biosynthesis SPODOPTERA SPODOPTERA EXIGUA STRUCTURAL GENES TEST BIOLOGIQUE Tobacco TOXINAS BACTERIANAS TOXINE BACTERIENNE TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transgenic insects TRANSGENIC PLANTS
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