Detection of gluten-containing cereals in food by 5-nuclease real-time polymerase chain reaction

A real-time polymerase chain reaction (PCR)-based method for the detection of coeliac disease-causing gluten-containing cereals (wheat, barley and rye) in food is described. The method consists of DNA isolation by chaotropic solid phase extraction and the subsequent real-time PCR with primers and a...

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Veröffentlicht in:	Journal of food and nutrition research 2008, Vol.47 (3), p.114-119
Hauptverfasser:	Piknová, Ľ., Food Research Institute, Bratislava (Slovak Republic), Brežná, B., Food Research Institute, Bratislava (Slovak Republic), Kuchta, T., Food Research Institute, Bratislava (Slovak Republic)
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN BARLEY BLE CEBADA CENTENO DNA http://www.fao.org/aos/agrovoc#c_14010 http://www.fao.org/aos/agrovoc#c_2347 http://www.fao.org/aos/agrovoc#c_33096 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_823 http://www.fao.org/aos/agrovoc#c_8373 ORGE PCR REPUBLICA ESLOVACA REPUBLIQUE SLOVAQUE RYE SEIGLE SLOVAK REPUBLIC TRIGO WHEATS
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