Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety
Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magn...
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creator | Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Shin, H.C., Korea Testing and Research Institute, Kimpo, Republic of Korea Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Kim, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea |
description | Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-EC∧50 values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects. |
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For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-EC∧50 values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.</description><identifier>ISSN: 1976-0442</identifier><identifier>DOI: 10.3839/jabc.2011.048</identifier><language>kor</language><subject>Bacillus thuringiensis rice ; DAPHNIA MAGNA ; Environment risk</subject><ispartof>Journal of applied biological chemistry, 2011-12, Vol.54 (4)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Shin, H.C., Korea Testing and Research Institute, Kimpo, Republic of Korea</creatorcontrib><creatorcontrib>Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Kim, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><title>Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety</title><title>Journal of applied biological chemistry</title><description>Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-EC∧50 values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.</description><subject>Bacillus thuringiensis rice</subject><subject>DAPHNIA MAGNA</subject><subject>Environment risk</subject><issn>1976-0442</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNpdkEtLw0AYRWehYK0uXQqz1EXqvJKZuGv6ULEglOK2fPNIOiWdlMxU8H_4g43oytWFC_ccuAjdUDLhipcPe9BmwgilEyLUGRrRUhYZEYJdoMsY94QUlCkyQl-LD2hPkHwXMASLpzG6GA8uJNzVuPJdhNqlT1x3Pa7A-LY9RZx2p96HxrsQfcR3VbrPUg8hNi54g9feuEe8dvHYhQH2w5nDcRc84AM0AfDSWTzoqvR_hd-h94PtCp3X0EZ3_ZdjtFkuNrPnbPX29DKbrrK6ZCkTJlea6kJJLYyhUilZMpvnljNbW-20lmJotOVCGciVMoznUlpJrSsKLvgY3f5ia-i20PQ-bl_Xw2eMEJ4zxr8BJcBkKw</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Shin, H.C., Korea Testing and Research Institute, Kimpo, Republic of Korea</creator><creator>Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Kim, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><creator>Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creator><scope>FBQ</scope></search><sort><creationdate>201112</creationdate><title>Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety</title><author>Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Shin, H.C., Korea Testing and Research Institute, Kimpo, Republic of Korea ; Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Kim, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea ; Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f92t-4c58b1b687b4cc1788792d55d32dfdbebb74792bd348ca588c23577d71de66343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2011</creationdate><topic>Bacillus thuringiensis rice</topic><topic>DAPHNIA MAGNA</topic><topic>Environment risk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Shin, H.C., Korea Testing and Research Institute, Kimpo, Republic of Korea</creatorcontrib><creatorcontrib>Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Kim, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><creatorcontrib>Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</creatorcontrib><collection>AGRIS</collection><jtitle>Journal of applied biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oh, S.D., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Shin, H.C., Korea Testing and Research Institute, Kimpo, Republic of Korea</au><au>Sohn, S.I., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Lee, K.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Kim, H.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Ryu, T.H., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Lee, J.Y., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Park, B.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Kweon, S.J., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Suh, S.C., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><au>Park, J.S., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety</atitle><jtitle>Journal of applied biological chemistry</jtitle><date>2011-12</date><risdate>2011</risdate><volume>54</volume><issue>4</issue><issn>1976-0442</issn><abstract>Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-EC∧50 values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn't affect to non-target insects.</abstract><doi>10.3839/jabc.2011.048</doi></addata></record> |
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subjects | Bacillus thuringiensis rice DAPHNIA MAGNA Environment risk |
title | Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety |
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