Identification of strain harboring both aac(6')-Ib and aac(6')-Ib-cr variant simultaneously in Escherichia coil and Klebsiella pneumoniae
The aac(6')-Ib gene is the most prevalent gene that encodes aminoglycoside-modifying enzymes and confers resistance to tobramycin, kanamycin, and amikacin. The aac(6')-Ib-cr variant gene can induce resistance against aminoglycoside and fluoroquinolone simultaneously. Two main methods, sequ...
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| creator | Kim, Y.T., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea Jang, J.H., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea Kim, H.C., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea Kim, H.G., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea Lee, K.R., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea Park, K.S., Kyung Hee University, Seoul, Republic of Korea Lee, H.J., Kyung Hee University, Seoul, Republic of Korea Kim, Y.J., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea |
| description | The aac(6')-Ib gene is the most prevalent gene that encodes aminoglycoside-modifying enzymes and confers resistance to tobramycin, kanamycin, and amikacin. The aac(6')-Ib-cr variant gene can induce resistance against aminoglycoside and fluoroquinolone simultaneously. Two main methods, sequence analysis and the restriction enzyme method, can detect the aac(6')-Ib-cr variant in clinical strains. We collected the 85 strains that were believed to be aac(6')-Ib positive from clinical isolates. Among them, 38 strains were the wild-type; the remaining 47 strains were the aac(6')-Ib-cr variant Of these 47 strains, 19 simultaneously harbored aac(6')-Ib and aac(6')-Ib-cr. Our study aims to report the characteristics of the 19 strains that simultaneously harbored both genes. This study is the first investigation published in Korea of strains that included both aac(6')-Ib and aac(6')-Ib-cr variant. |
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The aac(6')-Ib-cr variant gene can induce resistance against aminoglycoside and fluoroquinolone simultaneously. Two main methods, sequence analysis and the restriction enzyme method, can detect the aac(6')-Ib-cr variant in clinical strains. We collected the 85 strains that were believed to be aac(6')-Ib positive from clinical isolates. Among them, 38 strains were the wild-type; the remaining 47 strains were the aac(6')-Ib-cr variant Of these 47 strains, 19 simultaneously harbored aac(6')-Ib and aac(6')-Ib-cr. Our study aims to report the characteristics of the 19 strains that simultaneously harbored both genes. 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The aac(6')-Ib-cr variant gene can induce resistance against aminoglycoside and fluoroquinolone simultaneously. Two main methods, sequence analysis and the restriction enzyme method, can detect the aac(6')-Ib-cr variant in clinical strains. We collected the 85 strains that were believed to be aac(6')-Ib positive from clinical isolates. Among them, 38 strains were the wild-type; the remaining 47 strains were the aac(6')-Ib-cr variant Of these 47 strains, 19 simultaneously harbored aac(6')-Ib and aac(6')-Ib-cr. Our study aims to report the characteristics of the 19 strains that simultaneously harbored both genes. 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Jang, J.H., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea ; Kim, H.C., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea ; Kim, H.G., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea ; Lee, K.R., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea ; Park, K.S., Kyung Hee University, Seoul, Republic of Korea ; Lee, H.J., Kyung Hee University, Seoul, Republic of Korea ; Kim, Y.J., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-fao_agris_KR20120017143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>DHPLC</topic><topic>ENZIMA DE RESTRICCION</topic><topic>ENZYME DE RESTRICTION</topic><topic>RESTRICTION ENZYMES</topic><topic>Sequence analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Y.T., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Jang, J.H., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Kim, H.C., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Kim, H.G., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Lee, K.R., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Park, K.S., Kyung Hee University, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Lee, H.J., Kyung Hee University, Seoul, Republic of Korea</creatorcontrib><creatorcontrib>Kim, Y.J., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</creatorcontrib><collection>AGRIS</collection><jtitle>BMB reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Y.T., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</au><au>Jang, J.H., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</au><au>Kim, H.C., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</au><au>Kim, H.G., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</au><au>Lee, K.R., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</au><au>Park, K.S., Kyung Hee University, Seoul, Republic of Korea</au><au>Lee, H.J., Kyung Hee University, Seoul, Republic of Korea</au><au>Kim, Y.J., Seoul Medical Science Institute/Seoul Clinical Laboratories, Seoul, Republic of Korea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of strain harboring both aac(6')-Ib and aac(6')-Ib-cr variant simultaneously in Escherichia coil and Klebsiella pneumoniae</atitle><jtitle>BMB reports</jtitle><date>2011-04</date><risdate>2011</risdate><volume>44</volume><issue>4</issue><issn>1976-6696</issn><abstract>The aac(6')-Ib gene is the most prevalent gene that encodes aminoglycoside-modifying enzymes and confers resistance to tobramycin, kanamycin, and amikacin. The aac(6')-Ib-cr variant gene can induce resistance against aminoglycoside and fluoroquinolone simultaneously. Two main methods, sequence analysis and the restriction enzyme method, can detect the aac(6')-Ib-cr variant in clinical strains. We collected the 85 strains that were believed to be aac(6')-Ib positive from clinical isolates. Among them, 38 strains were the wild-type; the remaining 47 strains were the aac(6')-Ib-cr variant Of these 47 strains, 19 simultaneously harbored aac(6')-Ib and aac(6')-Ib-cr. Our study aims to report the characteristics of the 19 strains that simultaneously harbored both genes. This study is the first investigation published in Korea of strains that included both aac(6')-Ib and aac(6')-Ib-cr variant.</abstract></addata></record> |
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| subjects | DHPLC ENZIMA DE RESTRICCION ENZYME DE RESTRICTION RESTRICTION ENZYMES Sequence analysis |
| title | Identification of strain harboring both aac(6')-Ib and aac(6')-Ib-cr variant simultaneously in Escherichia coil and Klebsiella pneumoniae |
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