Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis
Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal...
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| Veröffentlicht in: | Journal of Plant Biotechnology 2007-03, Vol.34 (1) |
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| container_title | Journal of Plant Biotechnology |
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| creator | Oh, M.J. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) Min, S.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) Liu, J.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) Kim, S.W. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: kimsw@kribb.re.kr |
| description | Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2MS medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. |
| format | Article |
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(Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Min, S.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Liu, J.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Kim, S.W. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: kimsw@kribb.re.kr</creator><creatorcontrib>Oh, M.J. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Min, S.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Liu, J.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) ; Kim, S.W. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: kimsw@kribb.re.kr</creatorcontrib><description>Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2MS medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). 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The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2MS medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation.</description><subject>EMBRIOGENESIS SOMATICA</subject><subject>EMBRYOGENESE SOMATIQUE</subject><subject>SOMATIC EMBRYOGENESIS</subject><issn>1598-6365</issn><issn>2384-1397</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFjMkKwjAUAIMoWJdPEN5RDy1pa7qcRREUldZ7iZpqNIskqaBfr4J3T8MwMC3kRXE29cM4T9vIC0me-UmckC7qWXvFeEpSTDx02wmqHBTszBQz1HGtoDZawkJoQwWUjkmYNcI1hlnQNWye8n7R_PQxrk78SGG8DiawDWDVKPdiATw4hVLLz-sIc3kwT_19W24HqFNTYdnwxz4aLeb72dKvqa7o2XBbrYoI4wzjKCVh_K-_AR6iQsE</recordid><startdate>200703</startdate><enddate>200703</enddate><creator>Oh, M.J. 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(Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: kimsw@kribb.re.kr</creatorcontrib><collection>AGRIS</collection><jtitle>Journal of Plant Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oh, M.J. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea)</au><au>Min, S.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea)</au><au>Liu, J.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea)</au><au>Kim, S.W. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: kimsw@kribb.re.kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis</atitle><jtitle>Journal of Plant Biotechnology</jtitle><date>2007-03</date><risdate>2007</risdate><volume>34</volume><issue>1</issue><issn>1598-6365</issn><eissn>2384-1397</eissn><abstract>Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2MS medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation.</abstract></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | EMBRIOGENESIS SOMATICA EMBRYOGENESE SOMATIQUE SOMATIC EMBRYOGENESIS |
| title | Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis |
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